Inactivation of bacterial endotoxin in water using a pressurized microfluidic ultra-high temperature (UHT) pasteurizer

Mohamed, Hussein M.H.; Burroughs, Samantha; Summerville, Steven; Kearsley, Keith; Rivas, Logan; Hu, Dean; Waite‐Cusic, Joy

doi:10.1016/j.jwpe.2022.102927

Cited by 1 publication

(1 citation statement)

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“…The quality and standard of limulus produced in the United States are the highest, the output is the largest, and the technical content of the products is also the highest. Although the specific limulus lysate has been successfully prepared by gene cloning and heterologous protein expression abroad, compared with the natural limulus lysate, the recombinant limulus lysate for the detection of endotoxins still has some problems such as low sensitivity, high production cost and different detection results [11] . The addition of antilipopolysaccharide to the lipopolysaccharide lysate could not completely block the interference of endotoxin to the lipopolysaccharide lysate test.…”

Section: Limulus Industry Statusmentioning

confidence: 99%

Research progress of limulus coagulation mechanism and limulus reagents

Yan,

Li,

Gao

2024

BIO Web Conf.

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The coagulation system of Limulus is mainly composed of coagulation proteins such as factor G, factor C, factor B, and prothrombin. The traditional horseshoe crab reagent for endotoxin detection is not specific, and its colorimetric reaction is activated through two pathways: one is activated by endotoxin to activate factor C, and the other is activated by (1,3)- β-D-glucan activates factor G. The horseshoe crab reagent is composed of a series of serine proteases and is a biological reagent made by treating horseshoe crab blood cell lysate. The detection principle is that the Limulus amebocyte lysate clotting factor protein is activated by endotoxin or fungal glucan to generate a series of cascade reactions to form insoluble gel substances, and then it is used to detect bacterial endotoxin or fungal glucan contaminated in biological products through the chromogenic reaction of the chromogenic matrix. Mainly used in multiple fields such as drug inspection, food, medical equipment, clinical medical research, etc. This article mainly summarizes and summarizes the coagulation mechanism of horseshoe crab and the current development status of horseshoe crab reagents.

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Section: Limulus Industry Statusmentioning

confidence: 99%