Inactivation of Bacillus subtilis spores at various germination and outgrowth stages using intense pulsed light

Jo, Hye Lim; Shin, Han Seung; Chung, Myong Soo

doi:10.1016/j.fm.2019.03.013

Cited by 17 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IPL device used in this study was self-designed and the device consists of a power supply, treatment chamber, and xenon lamp (NL4006, XAP series, Heraeus Noblelight, Cambridge, UK), which emits light at wavelengths from 200 to 1100 nm [ 29 ]. Before the IPL treatment, 20 g of dried green tea leaves were immersed in 250 mL of tap water, and then sieved to remove water as much as possible.…”

Section: Methodsmentioning

confidence: 99%

Improving the Extraction of Catechins of Green Tea (Camellia sinensis) by Subcritical Water Extraction (SWE) Combined with Pulsed Electric Field (PEF) or Intense Pulsed Light (IPL) Pretreatment

Shin

Kim

Chung

2021

Foods

Self Cite

View full text Add to dashboard Cite

The aim of this study was to find the optimum condition of pulsed electric field (PEF) and intense pulsed light (IPL) for the enhancement of subcritical water extraction (SWE), which is an eco-friendly extraction method, for extracting tea catechins from green tea leaves (Camellia sinensis). The leaves were treated with PEF under conditions of electric field strength (1, 2 and 3 kV/cm) during 60 s. Moreover, IPL was applied at various voltages (800, 1000, and 1200 V) for 60 s. The SWE was performed for 5 min at varying temperatures (110, 130, 150, 170, and 190 °C). The maximum yield of total catechin was 44.35 ± 2.00 mg/g dry green tea leaves at PEF treatment conditions of 2 kV/cm during 60 s, as well as the SWE temperature of 130 °C. In the case of IPL treatment, the largest amount of total catechin was 48.06 ± 5.03 mg/g dry green tea leaves at 800 V during 60 s when the extraction temperature was 130 °C. The total catechin content was increased by 15.43% for PEF and 25.09% for IPL compared to the value of untreated leaves. This study verified that PEF and IPL had a positive effect on the enhancement of tea catechins extraction from green tea leaves using SWE.

show abstract

Section: Methodsmentioning

confidence: 99%

Improving the Extraction of Catechins of Green Tea (Camellia sinensis) by Subcritical Water Extraction (SWE) Combined with Pulsed Electric Field (PEF) or Intense Pulsed Light (IPL) Pretreatment

Shin

Kim

Chung

2021

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…This strain is a nonpathogenic spoilage and spore-forming bacteria that has a low usage risk in the laboratory environment. B. subtilis was cultured in 3 mL of Bacto TM Brain Heart Infusion (Difco TM , BD, Sparks, MD, USA) broth at 30 °C for 24 h (Jo et al, 2019). Twenty microliters of the culture medium was inoculated on nutrient agar (Difco TM , BD) with added sporulation salt (100 mg/L CaCl 2 and 40 mg/L MnSO 4 ) at 30 °C.…”

Section: Spore Preparationmentioning

confidence: 99%

“…The self-designed laboratory-scale IPL treatment device consisted of a power supply, a cooling system, and a treatment chamber containing a xenon lamp which emits IPL with the 200-1100 nm range of wavelength, fans for ventilation, and a shelf whose distance from the lamp could be adjusted (Jo et al, 2019). The power supply was adjustable for processing time, pulse width (a measure of the time between the beginning and end of the pulse, typically based on the full width half maximum of the pulse shape), frequency, and charging voltage.…”

Section: Ipl Devicementioning

confidence: 99%

“…There are several factors that affect the higher resistance of the spore. First, there are the chemical and physical substances that affect the sensitivity to stress like dipicolinic acid, a/b-type small acid-soluble proteins, and spore coat (Clair et al, 2020;Jo et al, 2019;Setlow, 2006). In particular, when light irradiation is performed when the microorganisms are in a spore state, photoproduct like pyrimidine dimers are better recovered due to their DNA repair mechanism (Setlow, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of factors affecting the efficacy of intense pulsed light for reducing Bacillus subtilis spores

Shin

Kim

Chung

2021

Food Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

This study investigated how the following four intense pulsed light (IPL) treatment factors affect the inactivation of Bacillus subtilis (KCCM 11,315) spores: distance between the sample and IPL lamp (8, 13, and 18 cm), pulse width (0.5, 1.3, and 2.1 ms), charging voltage (1000, 1200, and 1400 V), and processing time (10, 20, and 30 s). The results showed that all four factors considerably influenced the spore inactivation rate in different ways. Excluding processing time, which does not affect the pulse itself, the effect was largest for pulse width, followed by distance, and charging voltage. The optimal treatment condition that maximized the inactivation rate was a distance of 8 cm, a pulse width of 2.1 ms, a charging voltage of 1000 V, and a processing time of 30 s, which together produced a 6 log reduction. It revealed that individual factors need to be investigated together for achieving the optimal condition of IPL.

show abstract

“…In another study, intense pulsed light treatment at a total fluence of 7.40 J/cm 2 resulted in a 7 log reduction, indicating the potential of intense pulsed light to inactivate bacterial spores effectively. The best results showed that the inactivation efficiency increases after one h pre-incubation because the numbers of vegetative cells increased with the incubation time [15].…”

Section: Pulsed Lightmentioning

confidence: 99%

Emerging strategies for the development of food industries

et al. 2019

View full text Add to dashboard Cite

Undoubtedly, the food industry is undergoing a dynamic process of transformation in its continual development in order to meet the requirements and solve the great problems represented by a constantly growing global population and food claimant in both quantity and quality. In this sense, it is necessary to evaluate the technological trends and advances that will change the landscape of the food processing industry, highlighting the latest requirements for equipment functionality. In particular, it is crucial to evaluate the influence of sustainable green biotechnology-based technologies to consolidate the food industry of the future, today, and it must be done by analyzing the mega-consumption trends that shape the future of industry, which range from local sourcing to on-the-go food, to an increase in organic foods and clean labels (understanding ingredients on food labels). While these things may seem alien to food manufacturing, they have a considerable influence on the way products are manufactured. This paper reviews in detail the conditions of the food industry, and particularly analyzes the application of emerging technologies in food preservation, extraction of bioactive compounds, bioengineering tools and other biobased strategies for the development of the food industry.

show abstract

Inactivation of Bacillus subtilis spores at various germination and outgrowth stages using intense pulsed light

Cited by 17 publications

References 28 publications

Improving the Extraction of Catechins of Green Tea (Camellia sinensis) by Subcritical Water Extraction (SWE) Combined with Pulsed Electric Field (PEF) or Intense Pulsed Light (IPL) Pretreatment

Improving the Extraction of Catechins of Green Tea (Camellia sinensis) by Subcritical Water Extraction (SWE) Combined with Pulsed Electric Field (PEF) or Intense Pulsed Light (IPL) Pretreatment

Impact of factors affecting the efficacy of intense pulsed light for reducing Bacillus subtilis spores

Emerging strategies for the development of food industries

Contact Info

Product

Resources

About