Inactivation of alkene oxidation by epoxides in alkene-and alkane-grown bacteria

Use of “Specific” Inhibitors in Biogeochemistry and Microbial Ecology

“…These two compounds have the advantage of being relatively soluble, and their abundance can be easily measured by flame ionization gas chromatography. Another compound recently revealed as an inhibitor of methane monooxygenase is 1,2-epoxypropane (Habets-Crutzen and de Bont, 1985). This epoxide causes >95% inhibition of methane oxidation, but not methanol oxidation, by cell suspensions of Methyiosinus trichosporium OB3b.…”

Production of enantiopure styrene oxide by recombinantEscherichia coli synthesizing a two-component styrene monooxygenase

“…This is best realized with whole cells to facilitate cofactor regeneration. However, this approach has to overcome a number of challenges: The epoxide product might be toxic to the cell as well as inhibitory to the enzyme Habets-Crützen and de Bont, 1985;Prichamont et al, 1998). Also, the substrate of the reaction might be toxic to the cell (Wubbolts et al, 1994).…”

The effect of calcium alginate entrapment on the physiology of Mycobacterium sp. strain E3

“…1,2-Epoxypropane has also been shown to be toxic to Myeobacterium sp. E3 cells (Habets-CrOtzen and de Bont 1985) and this may offer an explanation for the present findings. However, the incubation of cells with and without sodium alginate in the absence of propene demonstrated a stim-645 ulation by sodium alginate on the endogenous production of CO2 (Fig.…”