Inactivation of AdeABC and AdeIJK efflux pumps elicits specific nonoverlapping transcriptional and phenotypic responses in <i>Acinetobacter baumannii</i>

Leus, Inga V.; Adamiak, Justyna; Trinh, Anhthu; Smith, Richard D.; Smith, Lauren A.; Richardson, Sophie; Ernst, Robert K.; Zgurskaya, Helen I.

doi:10.1111/mmi.14594

Cited by 33 publications

(53 citation statements)

References 58 publications

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“…Furthermore, it is documented that A . baumannii can use other efflux pump-associated genes [ 14 , 65 ] and superfamily [ 22 ] to develop a multidrug-resistance profile. In addition, researchers reported that the AdeABC efflux pump need, in some cases, a synergistic action with other resistance mechanisms to express a level of resistance to particular antibiotic classes [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…Literature supports that it is not just the presence of the AdeABC efflux pump-associated gene but their overexpression triggered by a mutation that induce resistance to antibiotics [15,63,64]. Furthermore, it is documented that A. baumannii can use other efflux pumpassociated genes [14,65] and superfamily [22] to develop a multidrug-resistance profile. In addition, researchers reported that the AdeABC efflux pump need, in some cases, a synergistic action with other resistance mechanisms to express a level of resistance to particular antibiotic classes [67].…”

Section: Discussionmentioning

confidence: 99%

“…baumannii genome is reported to contribute to antibiotic resistance in A . baumannii clinical isolates [ 22 ]. The overexpression of efflux pumps in A .…”

Section: Introductionmentioning

confidence: 99%

“…rough this mechanism, the bacteria avoid accumulation of drugs at the targeted site within it, leading to decreased susceptibility to antibiotics [17,21]. ree resistance-nodulation-cells division encoded in A. baumannii genome is reported to contribute to antibiotic resistance in A. baumannii clinical isolates [22].…”

Section: Introductionmentioning

confidence: 99%

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Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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Acinetobacter baumannii (A. baumannii) has developed several resistance mechanisms. The bacteria have been reported as origin of multiple outbreaks. This study aims to investigate the use of efflux pumps and quinolone resistance-associated genotypic mutations as mechanisms of resistance in A. baumannii isolates at a tertiary hospital. A total number of 103 A. baumannii isolates were investigated after identification and antimicrobial susceptibility testing by VITEK2 followed by PCR amplification of blaOXA-51. Conventional PCR amplification of the AdeABC efflux pump (adeB, adeS, and adeR) and quinolone (parC and gyrA) resistance genes were performed, followed by quantitative real-time PCR of AdeABC efflux pump genes. Phenotypic evaluation of efflux pump expression was performed by determining the difference between the MIC of tigecycline before and after exposure to an efflux pump inhibitor. The Sanger sequencing method was used to sequence the parC and gyrA amplicons. A phylogenetic tree was drawn using MEGA 4.0 to evaluate evolutionary relatedness of the strains. All the collected isolates were blaOXA-51-positive. High resistance to almost all the tested antibiotics was observed. Efflux pump was found in 75% of isolates as a mechanism of resistance. The study detected parC gene mutation in 60% and gyrA gene mutation in 85%, while 37% of isolates had mutations on both genes. A minimal evolutionary distance between the isolates was reported. The use of the AdeABC efflux pump system as an active mechanism of resistance combined with point mutation mainly in gyrA was shown to contribute to broaden the resistance spectrum of A. baumannii isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…baumannii genome is reported to contribute to antibiotic resistance in A . baumannii clinical isolates [ 22 ]. The overexpression of efflux pumps in A .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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“…The adeABC genes, encoding an RND family transporter, showed a 2–3 log2-fold increase in expression in the presence of any of the four molecules. This apparent induction by polyamines was surprising given that A. baumannii strain AB5075 carries a mutation in the regulator AdeS, which causes constitutive adeABC expression 32 , 33 . The MFS transporter gene amvA was induced by spermidine and spermine but not by putrescine and cadaverine.…”

Section: Resultsmentioning

confidence: 99%

The Acinetobacter baumannii disinfectant resistance protein, AmvA, is a spermidine and spermine efflux pump

et al. 2021

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Antimicrobial resistance genes, including multidrug efflux pumps, evolved long before the ubiquitous use of antimicrobials in medicine and infection control. Multidrug efflux pumps often transport metabolites, signals and host-derived molecules in addition to antibiotics or biocides. Understanding their ancestral physiological roles could inform the development of strategies to subvert their activity. In this study, we investigated the response of Acinetobacter baumannii to polyamines, a widespread, abundant class of amino acid-derived metabolites, which led us to identify long-chain polyamines as natural substrates of the disinfectant efflux pump AmvA. Loss of amvA dramatically reduced tolerance to long-chain polyamines, and these molecules induce expression of amvA through binding to its cognate regulator AmvR. A second clinically-important efflux pump, AdeABC, also contributed to polyamine tolerance. Our results suggest that the disinfectant resistance capability that allows A. baumannii to survive in hospitals may have evolutionary origins in the transport of polyamine metabolites.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Harvey

2023

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. The review is basically divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of arrays. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other areas such as medicine, industrial processes and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. The reported work shows increasing use of incorporation of new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented nearly 40 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show little sign of diminishing.

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Inactivation of AdeABC and AdeIJK efflux pumps elicits specific nonoverlapping transcriptional and phenotypic responses in Acinetobacter baumannii

Abstract: Acinetobacter baumannii is a Gram-negative coccobacillus that can cause a broad range of severe infections, including soft tissue and skin infections, urinary tract infections, and most significantly ventilator-associated pneumonia and bloodstream infections

Cited by 33 publications

References 58 publications

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

The Acinetobacter baumannii disinfectant resistance protein, AmvA, is a spermidine and spermine efflux pump

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

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