Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi

Wang, Ning; Tang, Chunlei; Fan, Xin; He, Mengying; Gan, Pengfei; Zhang, Shan; Hu, Zeyu; Wang, Xiaodong; Yan, Tao; Shu, Weixue; Yu, Ligang; Zhao, Jinren; He, Jia‐Ni; Li, Lili; Wang, Jianfeng; Huang, Xueling; Huang, Lili; Zhou, Jian‐Min; Zhen-sheng, Kang; Wang, Xiaojie

doi:10.1016/j.cell.2022.06.027

Cited by 90 publications

(78 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the MutRNA-seq approach helped us to identify a Pm69 candidate within an NLR cluster by sequencing the transcriptome of the resistant wild-type G305-3M and its susceptible derivative mutants (Figure 3). MutRNA-seq approach is a useful method for identifying candidate genes based on comparisons of a wild-type line with its derivative mutants 51–53 . The main advantage of this combination of long-read sequencing and RNAseq method is that it allows to anchor short DNA reads to the long ONT contigs allowing to determine if two fragments belong to the same gene or separate genes.…”

Section: Discussionmentioning

confidence: 99%

Long-read genome sequencing accelerated the cloning ofPm69by resolving the complexity of a rapidly evolving resistance gene cluster in wheat

Wei

Sela

et al. 2022

Preprint

View full text Add to dashboard Cite

Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks in the cloning of the powdery mildew (Pm) resistance gene (R-gene)Pm69derived from tetraploid wild emmer wheat (WEW). A conventional positional cloning approach encountered suppressed recombination due to structural variations, while chromosome sorting yielded an insufficient purity level. APm69physical map, constructed by assembling ONT long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster. A single candidate NLR was identified within this cluster by anchoring RNASeq reads of susceptible mutants to ONT contigs and was validated by the virus-induced gene silencing (VIGS) approach.Pm69, comprising Rx_N with RanGAP interaction sites, NB-ARC, and LRR domains, is probably a newly evolved NLR discovered only in one location across the WEW distribution range in the Fertile Crescent.Pm69was successfully introgressed into durum and bread wheat, and a diagnostic molecular marker could be used to accelerate its deployment and pyramiding with other resistance genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Long-read genome sequencing accelerated the cloning ofPm69by resolving the complexity of a rapidly evolving resistance gene cluster in wheat

Wei

Sela

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…This brief article highlights the recent results of Wang et al (2022), who found that knockout of a susceptibility gene increases resistance to stripe rust of wheat without yield penalty. Wheat, one of the most important and widely cultivated cereal crops, provides approximately 20% of the dietary calories and protein for humans worldwide (Shiferaw et al, 2013).…”

mentioning

confidence: 85%

Modification of the susceptibility gene TaPsIPK1 - a win-win for wheat disease resistance and yield

2022

View full text Add to dashboard Cite

Wheat is one of the most important cereal crops, and it is essential for worldwide food security. However, wheat production is threatened by various diseases, including wheat stripe rust caused by the fungus Puccinia striiformis f. sp. tritici (Pst). The development of plant resistance against disease is usually challenged by potential reduction in crop yield due to the enhancement of plant immunity. In a recent article, Wang et al. found that TaPsIPK1 is a susceptibility gene targeted by rust effectors. Editing of TaPsIPK1 increases resistance to stripe rust without any developmental effects or yield penalty, providing an exceptional resource for developing disease resistance in wheat.

show abstract

“…Moreover, recently developed base editors and prime editors allow for precise genome modifications. Collectively, these technologies have already had an impact on plant biological research and will play an important role in crop breeding [ 5 , 6 , 7 , 8 ].…”

Section: Crispr/cas: a Useful Genome Editing Toolmentioning

confidence: 99%

“…After a decade of development, CRISPR/Cas technology has become the most widely used gene editing tool in gene function research, gene therapy, and molecular breeding for crop improvement [ 3 , 4 ]. CRISPR/Cas technologies enable the precise and efficient genetic manipulation of various crop species; thus, they may be applied to crop improvement, agricultural breeding, and wild species domestication [ 5 , 6 , 7 ]. The CRISPR/Cas9 system can be used to create insertions or deletions (indels) in the coding region of target genes, leading to gene knock-out [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Virus-Induced Gene Editing and Its Applications in Plants

Zhang

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of plant genomes, have revolutionized plant science and enabled the creation of germplasms with beneficial traits. In order to apply these technologies, CRISPR/Cas reagents must be delivered into plant cells; however, this is limited by tissue culture challenges. Recently, viral vectors have been used to deliver CRISPR/Cas reagents into plant cells. Virus-induced genome editing (VIGE) has emerged as a powerful method with several advantages, including high editing efficiency and a simplified process for generating gene-edited DNA-free plants. Here, we briefly describe CRISPR/Cas-based genome editing. We then focus on VIGE systems and the types of viruses used currently for CRISPR/Cas9 cassette delivery and genome editing. We also highlight recent applications of and advances in VIGE in plants. Finally, we discuss the challenges and potential for VIGE in plants.

show abstract

Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi

Cited by 90 publications

References 60 publications

Long-read genome sequencing accelerated the cloning ofPm69by resolving the complexity of a rapidly evolving resistance gene cluster in wheat

Long-read genome sequencing accelerated the cloning ofPm69by resolving the complexity of a rapidly evolving resistance gene cluster in wheat

Modification of the susceptibility gene TaPsIPK1 - a win-win for wheat disease resistance and yield

Virus-Induced Gene Editing and Its Applications in Plants

Contact Info

Product

Resources

About