Inactivation of 14-3-3 Protein Exacerbates Cardiac Hypertrophy and Fibrosis through Enhanced Expression of Protein Kinase C .BETA.2 in Experimental Diabetes

Narasimman, Gurusamy; Watanabe, Kenichi; Ma, Minghong; Zhang, Shaosong; Muslin, Anthony J.; Kodama, Makoto; Aizawa, Yoshifusa

doi:10.1248/bpb.28.957

Cited by 30 publications

(24 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14-3-3 proteins can interact with a vast array of different proteins and play a role in diverse functions like cell cycle regulation, signaling transduction and apoptosis [47]. A recent study revealed that inactivation of 14-3-3 proteins exacerbated cardiac hypertrophy and fibrosis in diabetic mice [48]. The role of 14-3-3 in asthma pathogenesis is unknown.…”

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Chronically Inflamed Lungs in a Mouse Chronic Asthma Model

Wong¹,

Zhao²

2008

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Asthma is a chronic airway inflammatory disease characterized by airway wall remodeling. The mechanisms underlying airway remodeling in asthma are not fully understood. There is an urgent need to investigate global protein profiling of chronically inflamed lungs to identify novel pathogenic molecules and biomarkers for chronic asthma. In this study, we described the first differentially expressed proteome of lung tissue and bronchoalveolar lavage fluid from a mouse chronic asthma model. Methods: BALB/c mice sensitized to ovalbumin were challenged with ovalbumin aerosol 3 times per week for 8 weeks. The lung tissue and lavage fluid proteins were resolved by 2-dimensional gel electrophoresis, and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. Results: Airway goblet cell hyperplasia, smooth muscle hyperplasia, subepithelial fibrosis, airway hyperresponsiveness, pulmonary inflammatory cell infiltration and elevated serum ovalbumin-specific IgE level were observed in our chronic asthma model. We have identified at least 100 protein spots that were differentially expressed in chronically inflamed lungs, and the identity of 66 protein spots was confirmed. Conclusions: Many of these proteins, including cytoskeleton-related proteins, Ca²⁺-binding proteins and anti-oxidant proteins, may be related to the development of airway remodeling, and they should be evaluated further as potential therapeutic targets and biomarkers for chronic asthma.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteome Analysis of Chronically Inflamed Lungs in a Mouse Chronic Asthma Model

Wong¹,

Zhao²

2008

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…In the context of hyperglycemia or insulin resistance, a model should exhibit (1) evidence of LV dysfunction such as 106,148,149 There are numerous models of enhanced cardiac fibrosis in the literature. Of relevance to diabetes are models with increased expression or activation of the renin-angiotensin signaling pathways.…”

Section: Minimal Criteria For Mouse Models Of Diabetic Cardiomyopathymentioning

confidence: 99%

Recipes for Creating Animal Models of Diabetic Cardiovascular Disease

Hsueh

Abel

Breslow

et al. 2007

Circulation Research

202

164

View full text Add to dashboard Cite

Abstract-For more than 50 years, investigators have unsuccessfully tried to recreate in experimental animals the cardiovascular complications of diabetes seen in humans. In particular, accelerated atherosclerosis and dilated cardiomyopathy, the major causes of mortality in patients with diabetes, have been conspicuously absent in many mouse models of the disease. Under the auspices of the NIH, the Animal Models of Diabetic Complications Consortium has worked to address this issue. This effort has focused on the development of mouse models because of the high level of genomic information available and the many well-developed genetic manipulations that may be performed in mice. Importantly, the consortium has also worked to standardize many methods to assess metabolic and cardiovascular end points for measurement of the diabetic state and its macrovascular complications. Finally, for maximum benefits from these animal models in the study of atherosclerosis and of other diabetic complications, the consortium has created a system for sharing both the animal models and the accumulated phenotypic data with the greater scientific community.

show abstract

“…The 14-3-3 proteins specifically bind to serine-phosphorylated proteins and interact with Raf-1, PI-3K, ASK-1, PKC or other protein kinases, regulating signalling pathways (18)(19)(20)(21). Previous studies demonstrated that the expression of 14-3-3 proteins β, γ, δ and θ is high in lung cancer tissue and are associated with malignant potential (22).…”

Section: Resultsmentioning

confidence: 99%

Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research

Tang

Zhao

et al. 2013

Biomedical Reports

View full text Add to dashboard Cite

Abstract. Liver cancer is a common malignant disease, with high incidence and mortality rates. The study on the proteomics of liver cancer has attracted particular attention. The quantitative study method of proteomics depends predominantly on two-dimensional (2D) gel electrophoresis. In the present study we reported a rapid and accurate proteomics quantitative study method of high repeatability that includes the use of stable isotope labeling for the extraction of proteins and peptides via enzymolysis to achieve new type 2D capillary liquid chromatography-mass spectrometry separation using the separation mode of cation-exchange chromatography in conjunction with reversed-phase chromatography. LTQ OrbiTrap mass spectrometry detection was also performed. A total of 188 differential proteins were analyzed, including 122 upregulating [deuterium/hydrogen ratio (D/H) >1.5)] and 66 downregulating proteins (D/H<0.67). These proteins may play an important role in the occurrence, drug resistance, metastasis and recurrence of cancer or other pathological processes. Such a proteomics technology may provide biological data as well as a new methodological basis for liver cancer research.

show abstract

Inactivation of 14-3-3 Protein Exacerbates Cardiac Hypertrophy and Fibrosis through Enhanced Expression of Protein Kinase C .BETA.2 in Experimental Diabetes

Cited by 30 publications

References 42 publications

Proteome Analysis of Chronically Inflamed Lungs in a Mouse Chronic Asthma Model

Proteome Analysis of Chronically Inflamed Lungs in a Mouse Chronic Asthma Model

Recipes for Creating Animal Models of Diabetic Cardiovascular Disease

Stable isotope dimethyl labeling combined with LTQ mass spectrometric detection, a quantitative proteomics technology used in liver cancer research

Contact Info

Product

Resources

About