Inactivation methods of soybean trypsin inhibitor – A review

Vagadia, Brinda Harish; Vanga, Sai Kranthi; Raghavan, Vijaya

doi:10.1016/j.tifs.2017.02.003

Cited by 146 publications

(92 citation statements)

References 91 publications

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“…This is in accordance with the results of Zarkadas et al (2007), who analyzed the AA composition of 14 soybean cultivars and found that they were high in glutamic acid but limited in methionine and cysteine. The trypsin inhibitors belong to the family of serine protease inhibitors, and function through complexing with trypsin and chymotrypsin, resulting in interference of the protein digestive process (Vagadia, Vanga, & Raghavan, 2017). Similar to our results, Latif, Pfannstiel, Makkar, and Becker (2013) reported that the AA composition of SF from EAE of peanuts was close to that of the untreated ground seed.…”

Section: Amino Acid (Aa) Composition and Antinutritional Factors Ofsupporting

confidence: 88%

Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE) of soybeans by dead‐end ultrafiltration

Zhang

Wang

et al. 2019

Food Science & Nutrition

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The skim fraction (SF) obtained from enzyme‐assisted aqueous extraction (EAE) of soybeans is a by‐product with high protein content of up to 60.67%. As such, it is of great interest to develop an efficient method to recover protein from this fraction. In this study, the potential of dead‐end ultrafiltration (UF) in recovering skim protein extracted with different proteases was evaluated. Two polyethersulfone (PES) membranes with molecular weight cutoffs (MWCO) of 3 kDa and 5 kDa were utilized. Results revealed that the membrane with the MWCO of 5 kDa exhibited better filtration efficiency, since higher permeate flux values and lower impurity rejections were observed. Compared with Flavourzyme and Protex 7L, Alcalase 2.4L and Protex 6L exhibited stronger hydrolyzing ability, resulting in higher filtration fluxes but lower protein rejection coefficients. The recovered protein showed comparable amino acid profile to SPC, while with significantly reduced levels of trypsin inhibitors and phytate (p < 0.05), indicating high quality of the recovered protein. Overall, UF can be applicable to recover high value‐added protein from EAE of soybeans and remove undesired components from the resulting protein products.

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Section: Amino Acid (Aa) Composition and Antinutritional Factors Ofsupporting

confidence: 88%

Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE) of soybeans by dead‐end ultrafiltration

Zhang

Wang

et al. 2019

Food Science & Nutrition

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“…However, feed costs, which constitute about 80% of total cost of production, remain a major challenge in poultry farming owing to the use of expensive feed ingredients during feed formulation (Elagib et al, 2013). Soybean and full-fat soybean products are commonly used to provide high-quality protein (Woyengo et al, 2014;Beski et al, 2015;Vagadia et al, 2017) for quail. However, the demand for soybean on the world market is high owing to competition between human beings and animals for this dietary protein source .…”

Section: Introductionmentioning

confidence: 99%

Exogenous carbohydrases do not improve the physiological and meat quality parameters of female Japanese quail fed canola-based diets

Mnisi¹,

Mlambo²,

Phatudi³

et al. 2017

SA J. An. Sci.

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In an internally controlled environment, a feeding trial using 210 six-week-old female Japanese quail (189.63 ± 11.891 g liveweight) was conducted to evaluate the effect of carbohydrase-treated (endo-1.4-betaxylanase 5600 TXU/g and endo-1.4-beta-glucanase 2500 TGU/g) canola-based diets on growth performance, haemo-biochemical parameters, carcass characteristics, and meat quality traits. Five isocaloric and isonitrogenous experimental diets were formulated: the control diet (CON) (commercial growers diet with no canola meal (CM) included); the control diet in which 17.5% of soybean meal was replaced with CM (CM0); and the CM0 diet in which a carbohydrase multi-enzyme was added at a rate of 5%, 10% and 15% (CM50, CM100 and CM150, respectively). Diets and clean water were offered ad libitum during the fourweek experimental period. Average weekly feed intake (AWFI) and average weekly weight gain (AWG) were used to calculate feed conversion efficiency (FCE). In week 7, no dietary influence was observed on AWFI. In week 8 and week 9, CON stimulated lower AWFI compared with diet CM100. Diets had no significant influence on AWG, FCE, and haemo-biochemical parameters of Japanese quail. Adding carbohydrases had no significant effect on internal organs, carcass and meat quality traits of quail. It was therefore concluded that inclusion of exogenous carbohydrases alone did not improve the utilization of a canola meal-based quail diet. However, there is a possibility that utilization of higher canola levels would be enhanced through multienzyme combinations. ______________________________________________________________________________________

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“…Although these two assisted extraction techniques have been reported to improve the extraction of bioactives from plant materials [18], it was not the case for the extraction of trypsin inhibitors from the Gac seeds (Figure 1). In fact, it appears that the ultrasound and microwave treatments had a negative impact on the Gac seed trypsin inhibitors as has previously been observed for trypsin inhibitors in soybeans [31,32] and in bean seeds [33].…”

Section: Effect Of Extraction Methods On the Trypsin Inhibitor Yieldmentioning

confidence: 62%

Effect of Solvents and Extraction Methods on Recovery of Bioactive Compounds from Defatted Gac (Momordica cochinchinensis Spreng.) Seeds

Parks

Nguyen

et al. 2018

Separations

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Gac (Momordica cochinchinensis Spreng.) seeds contain bioactive compounds with medicinal properties. This study aimed to determine a suitable solvent and extraction technique for recovery of important compounds, namely, trypsin inhibitors, saponins, and phenolics. The antioxidant capacity and total solids of derived extracts were also measured. Water with conventional extraction method gave the highest value of trypsin inhibitor activity (118.45 ± 4.90 mg trypsin g −1 ) while water-saturated n-butanol and methanol extracts were characterized by their highest content of saponins (40.75 ± 0.31 and 38.80 ± 2.82 mg AE g −1 , respectively). Aqueous extract with microwave assistance achieved the highest phenolics (3.18 ± 0.04 mg GAE g −1 ). As a measure of antioxidant capacity, the 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay gave highest value to the aqueous microwave extract (23.56 ± 0.82 µmol TE g −1 ) while the ferric reducing antioxidant power (FRAP) assay gave highest values to water-saturated n-butanol and 70% ethanol extracts (5.25 ± 0.04 and 4.71 ± 0.39 µmol TE g −1 , respectively). The total solids value was highest using water with microwave assistance (141.5 g kg −1 ) while ultrasound treatment did not improve any extractions. Therefore, trypsin inhibitors are suitably recovered using water while water-saturated n-butanol or methanol is for saponins, both using a conventional method. Microwave extraction is suitable for phenolics recovery. These conditions are recommended for an efficient recovery of bioactive compounds from defatted Gac seeds.

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Inactivation methods of soybean trypsin inhibitor – A review

Cited by 146 publications

References 91 publications

Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE) of soybeans by dead‐end ultrafiltration

Recovery of high value‐added protein from enzyme‐assisted aqueous extraction (EAE) of soybeans by dead‐end ultrafiltration

Exogenous carbohydrases do not improve the physiological and meat quality parameters of female Japanese quail fed canola-based diets

Effect of Solvents and Extraction Methods on Recovery of Bioactive Compounds from Defatted Gac (Momordica cochinchinensis Spreng.) Seeds

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