Inactivation and mutagenesis on isolated DNA. I. Theory of inactivation of transforming DNA

Бреслер, С. Е.; Kalinin, V.L.; Перумов, Д. А.

doi:10.1016/0027-5107(67)90001-2

Cited by 32 publications

(5 citation statements)

References 11 publications

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“…3a. This result probably is anomalous, however, because two more samples with very similar initial size did not give a similar result (see Table 1, lines [1][2][3]. The mean ratio of final to initial strand sizes for the second through the seventh entries in Table 1 is 0.494.…”

Section: Conditions Of Uptake Integration Of Donormentioning

confidence: 94%

Transformation and Deoxyribonucleic Acid Size: Extent of Degradation on Entry Varies with Size of Donor

Morrison

Guild

1972

J Bacteriol

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The fate of label introduced as donor deoxyribonucleic acid (DNA) into competent cells of Diplococcus pneumoniae was determined immediately after entry at 25 C, as a function of the size of the donor DNA. Part of the label is found to be acid soluble, part has been incorporated into chromosomal DNA, apparently through reincorporation of degraded donor DNA, and part is found in single strands of length smaller than that of the input donor DNA strands. The last fraction apparently constitutes the precursor for integration of intact donor genetic markers and is referred to as the intact fraction. For large donor DNA the intact fraction contains over 80% of the total intracellular label, but the median strand length has been reduced to 2.2 × 10 6 daltons. For small donor molecules (1 × 10 5 to 6 × 10 5 daltons per strand) the fraction intact increases with donor size from 10 to 50% of the total intracellular label, and the median strand length of this fraction is half that of the donor strands. By combining these results with earlier data on the size dependence of the yield of transformants per unit of total intracellular donor label, we have calculated the probability that a marker in the intact fraction will be integrated, as a function of the length of the donor strand after entry. This probability has a linear dependence on strand length for activities below 40% of maximum, and extrapolates to zero activity at 77,000 daltons per strand.

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Section: Conditions Of Uptake Integration Of Donormentioning

confidence: 94%

Transformation and Deoxyribonucleic Acid Size: Extent of Degradation on Entry Varies with Size of Donor

Morrison

Guild

1972

J Bacteriol

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“…With reference to the dose reduction principle (Harm et al, 1971), [S,]/[S,] can be determined by the H. influenzae transformation assay. From experimental data (Rupert and Goodgal, 1960) and theoretical considerations (Bresler et al, 1964(Bresler et al, , 1967Rupert, 1968) it appeared that the UV inactivation curve of transforming DNA is inverse quadratic, obeying the expression:…”

mentioning

confidence: 99%

PHOTOREACTIVATING ENZYME FROM Streptomyces griseus—VI. ACTION SPECTRUM AND KINETICS OF PHOTOREACTIVATION

Eker

Hessels

Dekker

1986

Photochem & Photobiology

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Abstract— A high resolution action spectrum for photoreactivation was determined using purified photoreactivating enzyme from Streptomyces griseus. Conversion of pyrimidine dimers in UV‐irradiated DNA, the substrate for photoreactivating enzyme, was measured with a Haemophilus influenzae transformation assay. A high similarity was found between action spectrum (max. at 445 nm) and the long wavelength absorption band (max. at 443 nm)of photoreactivating enzyme. In addition to the400–470 nm region considerable photoreactivation was found with wavelengths between 280 and 320 nm. No evidence was obtained for the presence of nonenzymatic photoreactivation. Comparison of in vitro and in vivo action spectra revealed that the sharp peak at 313 nm found in vivo is probably the result of counteracting photoreactivation and inactivation effects. Comparison of the action spectrum with the absorption spectrum of 8‐hydroxy‐10‐methyl‐5‐deazaisoalloxazine in an aprotic dipolar solvent (which serves as a model for the 8‐hydroxy‐5‐deazaflavin chromophore in photoreactivating enzyme) indicates the possible presence of other chromophore(s) involved in the photorepair process. From kinetic measurements and flash experiments values were obtained for the rate constants of the photoreactivation reaction. The quantum yield of photoreactivation was estimated to be approximately 1.

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“…According to Bresler et al (7), transforming DNA is inactivated when the integrated segment of donor DNA bearing the marker selected for also contains an inactivating hit. The average number of hits (Z) per genetic target can be calculated from the transformation data: Z = 2(VS<7g -1).…”

Section: Resultsmentioning

confidence: 99%

“…The specific transforming activity of markers was calculated by normalization to DNA concentration, determined by the concentration of 3H-thymine in DNA samples. The number of inactivating hits was calculated with the formula of Bresler et al (7), in which the average number of hits per genetic target (Z) is given by Z = 2(V977 -1), where SO is the specific transforming activity of a marker in unirradiated DNA and S is the specific transforming activity of that marker in the treated sample.…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis

Hadden

Billen

1973

J Bacteriol

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The repair of ultraviolet (UV) damage in Bacillus subtilis W23T − has been studied by transformation with deoxyribonucleic acid (DNA) extracted from irradiated cells before and after repair. The extent of repair of genetic markers by donor cells after low or moderate doses of UV was found to be related only to the initial degree of inactivation. After a very high dose, further inactivation occurred, also in proportion to initial damage. In addition, the competent recipient cells were shown to repair approximately 75% of the damage in transforming DNA. The sensitivities of markers irradiated either in vivo or in vitro appeared to be related to map position, the more proximal markers showing a greater resistance to UV inactivation.

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Inactivation and mutagenesis on isolated DNA. I. Theory of inactivation of transforming DNA

Cited by 32 publications

References 11 publications

Transformation and Deoxyribonucleic Acid Size: Extent of Degradation on Entry Varies with Size of Donor

Transformation and Deoxyribonucleic Acid Size: Extent of Degradation on Entry Varies with Size of Donor

PHOTOREACTIVATING ENZYME FROM Streptomyces griseus—VI. ACTION SPECTRUM AND KINETICS OF PHOTOREACTIVATION

Genetic Analysis of Repair of Ultraviolet Damage by Competent and Noncompetent Cells of Bacillus subtilis

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