2020
DOI: 10.1101/2020.07.08.194613
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Inactivation analysis of SARS-CoV-2 by specimen transport media, nucleic acid extraction reagents, detergents and fixatives

Abstract: The COVID-19 pandemic has necessitated a multi-faceted rapid response by the scientific community, bringing researchers, health officials and industry together to address the ongoing public health emergency. To meet this challenge, participants need an informed approach for working safely with the etiological agent, the novel human coronavirus SARS-CoV-2. Work with infectious SARS-CoV-2 is currently restricted to high-containment laboratories, but material can be handled at a lower containment level af… Show more

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Cited by 23 publications
(45 citation statements)
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“…Combined nose and throat swab samples are preferably resuspended in 2 ml of SAMBA SARS-CoV collection (SCoV) buffer, which is provided with the kit. SAMBA SCoV buffer contains detergent and is at a low pH; it has been shown to inactivate SARS-CoV-2 when examined using a pseudovirus luciferase assay as previously described ( 11 ) and shows a 4.8-log 10 reduction in titer when tested with tissue culture fluid containing SARS-CoV-2 ( 12 ). However, the system can be used with Remel M4RT or MWE Virocult viral transport medium (VTM) if the sample is diluted 1:2 with SCoV buffer prior to processing.…”
Section: Methodsmentioning
confidence: 99%
“…Combined nose and throat swab samples are preferably resuspended in 2 ml of SAMBA SARS-CoV collection (SCoV) buffer, which is provided with the kit. SAMBA SCoV buffer contains detergent and is at a low pH; it has been shown to inactivate SARS-CoV-2 when examined using a pseudovirus luciferase assay as previously described ( 11 ) and shows a 4.8-log 10 reduction in titer when tested with tissue culture fluid containing SARS-CoV-2 ( 12 ). However, the system can be used with Remel M4RT or MWE Virocult viral transport medium (VTM) if the sample is diluted 1:2 with SCoV buffer prior to processing.…”
Section: Methodsmentioning
confidence: 99%
“…Sixty nasopharyngeal swabs samples were collected from healthy volunteers using Rayon swab ( n = 30) or cotton swabs ( n = 30) and placed into cobas ® PCR Media tubes (Roche Molecular Systems, Inc. Basel, Switzerland) containing 4.3 mL of guanidine hydrochloride (<40%). Guanidine hydrochloride virtually inactivates all pathogens that may be present in the sample, preserves all nucleic acid of the specimen and protects the operator from contamination during sample manipulation [ 11 , 12 , 13 , 14 ]. Guanidine hydrochloride also guarantees the stability of the contrived samples, allowing us to introduce synthetic SARS-CoV-2 RNA into authentic nasopharyngeal specimens making the testing primary sample as real as possible.…”
Section: Methodsmentioning
confidence: 99%
“…The validation of 0.9% saline and PBS as an alternative transport medium for SARS-CoV-2 testing has been done by others [ 9 , 10 ]. The downside of 0.45% saline use instead of guanidine hydrochloride is that the former does not inactivate the microorganisms that may be present in the sample, and extra cautious should be applied during the sample measurement [ 7 ]. To our knowledge, this is the first description of use of 0.45% saline as a viral transport media for SARS-CoV-2 detection.…”
Section: Discussionmentioning
confidence: 99%
“…Additionally, we validated the sample collection in tubes containing guanidine hydrochloride. This viral transport media virtually inactivates the pathogens present in the sample and preserves the nucleic acid of the specimen [ 5 , 6 , 7 , 8 ], however, it this also ended up resulting in a short supply. It has been described that 0.9% saline and phosphate-buffered saline (PBS) are appropriated viral transport media for SARS-CoV-2 collection and testing during the shortage of commercials tubes containing a viral transport medium [ 9 , 10 ].…”
Section: Introductionmentioning
confidence: 99%