Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells

Chen, Jun; Han, Han; Chen, Min; Xu, Xiao-Zhu; Wang, Bin; Shi, Libin

doi:10.7314/apjcp.2014.15.12.5023

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Hoshyar et al showed that crocins initiated cell apoptosis by increasing caspase activity in human gastric adenocarcinoma cell line AGS (Hoshyar et al, 2013). It is well known that caspase-8 and caspase-9 are essential proteases of extrinsic and intrinsic apoptotic pathways, while caspase-3 acts as a downstream effector of these two pathways (Chen et al, 2014). Thus, our data suggested that saffron-induced apoptosis is caspase dependent and may involve in both the endogenous mitochondrial pathway and the exogenous death receptor pathway.…”

Section: Discussionsupporting

confidence: 59%

Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Liu¹,

Ye²,

Zhang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 59%

Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Liu¹,

Ye²,

Zhang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…26) There had been a lot of related reports about ghrelin interaction with LPS in different tissues and cells, and most previous studies found that ghrelin protected cells against LPS induced apoptosis, such as mouse dopaminergic neurones cell, 27) alveolar macrophages, 28) and oligodendrocyte cells. 29) Our results indicated that ghrelin can induce apoptosis of MH7A cells, while the effect of LPS for MH7A cells was puzzling. LPS not only increased cell viability of MH7A cells, but also induced cell apoptosis.…”

Section: Discussionmentioning

confidence: 67%

Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells

Wang

et al. 2019

Biological & Pharmaceutical Bulletin

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Ghrelin is a circulating peptide hormone, which involved in promoting feeding and regulating energy metabolism in human and rodents. Abnormal synovial hyperplasia is the most important pathologic hallmark of rheumatoid arthritis (RA), which is characterised by tumor-like expansion. Existing studies indicated that there may exist some relation between the decreased ghrelin and the abnormally proliferating synovial cells in RA. Therefore, the aim of this study is to explore the apoptotic effects of ghrelin on MH7A synovial cells in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the effects of ghrelin on the viability of MH7A cells. Terminal deoxynucleotidyl transferasemediated deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and flow cytometry were used to test the apoptotic effects of ghrelin. At last, Western blot and real-time PCR were performed to explore the expression of caspases-8,-9, and-3 after the treatment of ghrelin. MTT experiments showed that ghrelin could inhibit viability of MH7A cells. The results of flow cytometry and TUNEL showed that ghrelin could induce apoptosis of MH7A synovial cells. Western blot showed that expression of cleaved-caspases-8,-9, and-3 were increased in ghrelin stimulation group compared with the control group, while expression of pro-caspases-8,-9, and-3 had no significant difference. In mRNA levels, ghrelin can decrease pro-caspases-8,-9, and-3 mRNA expression, which confirmed the results of protein levels. Then these apoptotic effects were significantly reversed by [D-Lys 3 ] GHRP-6 (ghrelin receptor antagonist). This study found that ghrelin can induce apoptosis of MH7A cells through caspase signaling pathways.

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“…These results have suggested that ALEX1 promote the ratio of Bax/Bcl-2 and then result in cytochrome c release from mitochondria to cytosol. It is well known that caspase-8 and caspase-9 are essential proteases of extrinsic and intrinsic apoptotic pathways (Chen et al, 2014). Reports showed that the intrinsic apoptosis pathway is characterized as mitochondrial dysfunction and stimulation of caspase-9 and caspase-3 and caspase-3 is the cuultimate executioner for the nucleic changes with apoptosis (Chen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

ALEX1 Regulates Proliferation and Apoptosis in Breast Cancer Cells

Gao

Wu²,

Zeng³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Arm protein lost in epithelial cancers, on chromosome X (ALEX) is a novel subgroup within the armadillo (ARM) family, which has one or two ARM repeat domains as opposed to more than six-thirteen repeats in the classical Armadillo family members. Materials and Methods: In the study, we explore the biological functions of ALEX1 in breast cancer cells. Overexpression of ALEX1 and silencing of ALEX1 were performed with SK-BR3 and MCF-7 cell lines. Cell proliferation and colony formation assays, along with flow cytometry, were carried out to evaluate the roles of ALEX1. Results: ALEX1 overexpression in SK-BR3 breast cancer cells inhibited proliferation and induced apoptosis. Furthermore, depletion of ALEX1 in MCF-7 breast cancer cells increased proliferation and inhibited apoptosis. Additional analyses demonstrated that the overexpression of ALEX1 activated the intrinsic apoptosis cascades through up-regulating the expression of Bax, cytosol cytochrome c, active caspase-9 and active caspase-3 and down-regulating the levels of Bcl-2 and mitochondria cytochrome c. Simultaneouly, silencing of ALEX1 inhibited intrinsic apoptosis cascades through down-regulating the expression of Bax, cytosol cytochrome c, active caspase-9, and active caspase-3 and up-regulating the level of Bcl-2 and mitochondria cytochrome c. Conclusions: Our data suggest that ALEX1 as a crucial tumor suppressor gene has been involved in cell proliferation and apoptosis in breast cancer, which may serve as a novel candidate therapeutic target.

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Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells

Cited by 7 publications

References 32 publications

Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Distinct Pro-Apoptotic Properties of Zhejiang Saffron against Human Lung Cancer Via a Caspase-8-9-3 Cascade

Effects of Ghrelin on the Apoptosis of Rheumatoid Arthritis Fibroblast-Like Synoviocyte MH7A Cells

ALEX1 Regulates Proliferation and Apoptosis in Breast Cancer Cells

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