Inability of men mutants of Escherichia coli to use trimethylamine-N-oxide as an electron acceptor

Meganathan, R.

doi:10.1016/0378-1097(84)90340-9

Cited by 10 publications

(16 citation statements)

References 12 publications

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“…When the organism is grown with fumarate, TMAO, or dimethyl sulfoxide as the electron acceptor, the presence of menaquinone is obligatory (12,20,24). When oxygen or nitrate is the electron acceptor, E. coli uses the aerobic quinone, ubiquinone (17).…”

Section: Discussionmentioning

confidence: 99%

“…Although both quinones are made under both aerobic and anaerobic conditions, more ubiquinone than menaquinone is made aerobically and more menaquinone than ubiquinone is made anaerobically. It has been clearly shown that the utilization of O 2 and NO 3 is dependent on the presence of ubiquinone (17,20). Consequently, growth of menaquinone-deficient (men) mutants with NO 3 as the electron acceptor is unaffected (20).…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, growth of menaquinone-deficient (men) mutants with NO 3 as the electron acceptor is unaffected (20). In contrast, for the utilization of fumarate and TMAO, the presence of menaquinone is obligatory and, consequently, men mutants are unable to grow utilizing these compounds as electron acceptors (12,20).…”

Section: Discussionmentioning

confidence: 99%

“…It is well established that for the reduction of nitrate, ubiquinone is required (17), while menaquinone is obligatory for growth on fumarate and TMAO as electron acceptors (17,20). Hence, the entC ϩ strain was inoculated into media containing the oxidizable carbon source glycerol and nitrate, fumarate, or TMAO as electron acceptors under anaerobic conditions.…”

Section: Vol 178 1996 Entc Gene Expression and Vitamin K Biosynthesmentioning

confidence: 99%

“…As demonstrated above, the entC mutant was able to grow anaerobically on iron-sufficient media with fumarate or TMAO as electron acceptors. It is also well established that for the utilization of fumarate or TMAO as electron acceptors, the presence of menaquinone is obligatory (12,20). To verify the presence of menaquinone, we analyzed anaerobically grown cells of the entC mutant and those of its parent, AB1515, by established procedures (Materials and Methods).…”

Section: Vol 178 1996 Entc Gene Expression and Vitamin K Biosynthesmentioning

confidence: 99%

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Anaerobic biosynthesis of enterobactin Escherichia coli: regulation of entC gene expression and evidence against its involvement in menaquinone (vitamin K2) biosynthesis

1996

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In Escherichia coli, isochorismate is a common precursor for the biosynthesis of the siderophore enterobactin and menaquinone (vitamin K 2 ). Isochorismate is formed by the shikimate pathway from chorismate by the enzyme isochorismate synthase encoded by the entC gene. Since enterobactin is involved in the aerobic assimilation of iron, and menaquinone is involved in anaerobic electron transport, we investigated the regulation of entC by iron and oxygen. An operon fusion between entC with its associated regulatory region and lacZ ؉ was constructed and introduced into the chromosome in a single copy. Expression of entC-lacZ was found to be regulated by the concentration of iron both aerobically and anaerobically. An established entC::kan mutant deficient in enterobactin biosynthesis was found to grow normally and synthesize wild-type levels of menaquinone under anaerobic conditions in iron-sufficient media. These results led to the demonstration of an alternate isochorismate synthase specifically involved in menaquinone synthesis encoded by the menF gene. Consistent with these findings, the entC ؉ strains were found to synthesize enterobactin anaerobically under iron-deficient conditions while the ent mutants failed to do so.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Vol 178 1996 Entc Gene Expression and Vitamin K Biosynthesmentioning

confidence: 99%

Section: Vol 178 1996 Entc Gene Expression and Vitamin K Biosynthesmentioning

confidence: 99%

See 3 more Smart Citations

Anaerobic biosynthesis of enterobactin Escherichia coli: regulation of entC gene expression and evidence against its involvement in menaquinone (vitamin K2) biosynthesis

1996

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Dimethyl sulfoxide reductase is not required for trimethylamine N-oxide reduction in Escherichia chcoli

Daruwala

Meganathan

1991

FEMS Microbiology Letters

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Deletion mutants of Escherichia coli lacking dimethyl sulfoxide (DMSO) reductase activity and consequently unable to utilize DMSO as an electron acceptor for anaerobic growth have been isolated. These mutants retained the ability to use trimethylamine N-oxide (TMAO) as an electron acceptor and the TMAO reductase activity was found to be unaltered. Heating the cell-free extract of the wild-type strain at 70 degrees C for 15 min selectively inactivated the DMSO reductase activity while the TMAO reductase activity remained unchanged for at least 1 h.

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Role of menaquinone in the reduction of fumarate, nitrate, iron(III) and manganese(IV) by Shewanella putrefaciens MR-1

Myers

1993

FEMS Microbiology Letters

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Mutants of Shewanella putrefaciens MR-1 deficient in menaquinone and methylmenaquinone, but which have wild-type levels of ubiquinone, retain the ability to use trimethylamine N-oxide as an electron acceptor, but they lose the ability to use nitrate, iron(Ill), and fumarate as electron acceptors. These mutants also show a reduced rate of manganese(IV) reduction. One of these mutants could be restored to essentially wild-type phenotype by supplementing the medium with 1,4-dihydroxy-2-naphthoic acid. A requirement for naphthoquinones in iron(III) reduction and a preference for naphthoquinones in manganese(IV) reduction provide further support that the metal reducing systems in MR-1 are linked to anaerobic respiration.

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Inability of men mutants of Escherichia coli to use trimethylamine-N-oxide as an electron acceptor

Cited by 10 publications

References 12 publications

Anaerobic biosynthesis of enterobactin Escherichia coli: regulation of entC gene expression and evidence against its involvement in menaquinone (vitamin K2) biosynthesis

Anaerobic biosynthesis of enterobactin Escherichia coli: regulation of entC gene expression and evidence against its involvement in menaquinone (vitamin K2) biosynthesis

Dimethyl sulfoxide reductase is not required for trimethylamine N-oxide reduction in Escherichia chcoli

Role of menaquinone in the reduction of fumarate, nitrate, iron(III) and manganese(IV) by Shewanella putrefaciens MR-1

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