In Vivo Verification of Electron Paramagnetic Resonance Biodosimetry Using Patients Undergoing Radiation Therapy Treatment

Draeger, Emily; Roberts, Kenneth; Decker, Roy D.; Bahar, Nina; Wilson, Lynn D.; Contessa, Joseph; Husain, Zain; Williams, Benjamin B.; Flood, Ann Barry; Swartz, Harold M.; Carlson, David J.

doi:10.1016/j.ijrobp.2023.11.029

Cited by 1 publication

(1 citation statement)

References 34 publications

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“…In addition, a number of non-invasive spectroscopy-based methods for radiation biodosimetry purposes have been explored. For example, in vivo electron paramagnetic resonance biodosimetry has been used for biodosimetry in nail 41 , 42 , hair 43 and teeth 44 . Raman and photoluminescence (PL) spectroscopy were used to study radiation effects on human hair samples 45 .…”

Section: Introductionmentioning

confidence: 99%

Long-term, non-invasive FTIR detection of low-dose ionizing radiation exposure

Inman,

Wu,

Chen

et al. 2024

Sci Rep

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Non-invasive methods of detecting radiation exposure show promise to improve upon current approaches to biological dosimetry in ease, speed, and accuracy. Here we developed a pipeline that employs Fourier transform infrared (FTIR) spectroscopy in the mid-infrared spectrum to identify a signature of low dose ionizing radiation exposure in mouse ear pinnae over time. Mice exposed to 0.1 to 2 Gy total body irradiation were repeatedly measured by FTIR at the stratum corneum of the ear pinnae. We found significant discriminative power for all doses and time-points out to 90 days after exposure. Classification accuracy was maximized when testing 14 days after exposure (specificity > 0.9 with a sensitivity threshold of 0.9) and dropped by roughly 30% sensitivity at 90 days. Infrared frequencies point towards biological changes in DNA conformation, lipid oxidation and accumulation and shifts in protein secondary structure. Since only hundreds of samples were used to learn the highly discriminative signature, developing human-relevant diagnostic capabilities is likely feasible and this non-invasive procedure points toward rapid, non-invasive, and reagent-free biodosimetry applications at population scales.

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