In vivo ‘turn on’ fluorescence detection of free cysteine in zebrafish kidney and liver

Ghosh, Priyotosh; Mandal, Saurodeep; Kundu, Shampa; Saha, Shrabani; Sherpa, Rinchen D.; Islam, Ataul; Hui, Subhra Prakash; Mandal, Sukhendu; Sahoo, Prithidipa

doi:10.1016/j.jphotobiol.2023.112747

Cited by 2 publications

(1 citation statement)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cysteine (Cys) is known as a sulfur-containing nonessential amino acid. Being a key constituent of glutathione, this amino acid is involved in a lot of vital physiological functions such as protein synthesis, metabolism, and other processes. − It is mainly distributed in the organs (liver and kidney) and subcutaneous tissue. − In addition, it is also associated with development of many diseases, such as cerebrovascular disease (stroke) . Cys is normally oxidized to various essential compounds in the normal brain.…”

Section: Introductionmentioning

confidence: 99%

A Near-Infrared Fluorescent Dye with Tunable Emission Wavelength and Stokes Shift as a High-Sensitivity Cysteine Nanoprobe for Monitoring Ischemic Stroke

Wang,

Li,

et al. 2024

ACS Nano

View full text Add to dashboard Cite

Sulfur-substituted dicyanomethylene-4H-chromene (DCM) derivatives based on the intramolecular charge transfer (ICT) mechanism were designed as near-infrared (NIR) fluorescent dyes. Using the Knoevenagel condensation method, the S-DCM-OH (835) fluorescence dye was synthesized, which had an emission wavelength exceeding 800 nm and 220 nm of a Stokes shift. Compared to commercial ICG, S-DCM-OH (835) was not only synchronized in emission wavelength but also far superior in Stokes shifts. These advantages made the design of S-DCM-NIR (835) based on this dye potentially valuable for biological applications. Based on this chemical structure, a fluorescent S-DCM-NIR (835) nanoprobe with a mean diameter of 17.69 nm was fabricated as the NIR imaging nanoprobe. Results showed that the nanoprobe maintained the high-specificity identification of cysteine (Cys) via the Michael addition reaction, with the detection limitation of 0.11 μM endogenous Cys. More importantly, in an ischemic stroke mouse model, the S-DCM-NIR (835) nanoprobe could monitor the Cys concentration change at stroke lesion due to the disruption of Cys metabolism under the ischemic stroke condition. Such a S-DCM-NIR (835) nanoprobe could not only differentiate the severity of the ischemic stroke using response time but also quantify the concentration of Cys in real-time in vivo.

show abstract