In Vivo Tumorigenicity Assays Using Subcutaneous and Orthotopic Xenografts in Immunodeficient Mice

Yogi, Kedar; Thorat, Rahul; Shirsat, Neelam

doi:10.1007/978-1-0716-1952-0_15

Cited by 2 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, a subcutaneous transplanted model was used to evaluate the tumor growth inhibitory effect of sh‐circTOP2A in vivo. However, the model neglected factors such as the blood–brain barrier, a tissue microenvironment similar to that of clinical specimens 46 . Therefore, orthotopic xenografts can exhibit clinical characteristics such as in vivo invasion and metastatic patterns closely mimicking the glioma 46 .…”

Section: Discussionmentioning

confidence: 99%

“…However, the model neglected factors such as the blood–brain barrier, a tissue microenvironment similar to that of clinical specimens. 46 Therefore, orthotopic xenografts can exhibit clinical characteristics such as in vivo invasion and metastatic patterns closely mimicking the glioma. 46 This may be another limitation of our study.…”

Section: Discussionmentioning

confidence: 99%

“… 46 Therefore, orthotopic xenografts can exhibit clinical characteristics such as in vivo invasion and metastatic patterns closely mimicking the glioma. 46 This may be another limitation of our study.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

circTOP2A functions as a ceRNA to promote glioma progression by upregulating RPN2

Sun

Wang

et al. 2022

Cancer Science

View full text Add to dashboard Cite

Competing endogenous RNA (ceRNA)‐mediated signaling pathway dysregulation provides great insight into comprehensively understanding the molecular mechanism and combined targeted therapy for glioblastoma. circRNA is characterized by high stability, tissue/developmental stage‐specific expression and abundance in brain and plays significant roles in the initiation and progression of cancer. Our previous published data have demonstrated that RPN2 was significantly upregulated in glioma and promoted tumor progression via the activation of the Wnt/β‐catenin pathway. Furthermore, we proved that miR‐422a regulated the Wnt/β‐catenin signaling pathway by directly targeting RPN2. In this study, based on the glioblastoma microarray profiles, we identified the upstream circTOP2A, which completely bound to miR‐422a and was co‐expressed with the RPN2. circTOP2A was significantly overexpressed in glioma and conferred a poor prognosis. circTOP2A could regulate RPN2 expression by sponging miR‐422a, verified by western blot, dual‐luciferase reporter gene assay, and RNA pull‐down assay. Functional assays including CCK8, transwell and FITC‐annexin V were performed to explore the RPN2‐mediated role of the circTOP2A effect on the glioma malignant phenotype. Additionally, TOP/FOP and immunofluorescence analysis were used to confirm that sh‐circTOP2A could suppress the Wnt/β‐catenin pathway partly through RPN2. Finally, a tumor xenograft model was applied to validate the biological function of circTOP2A in vivo. Taken together, our findings reveal the critical role of circTOP2A in promoting glioma proliferation and invasion via a ceRNA mechanism and provide an exploitable biomarker and therapeutic target for glioma patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%