In vivo toxicity assessment of angiogenesis and the live distribution of nano-graphene oxide and its PEGylated derivatives using the developing zebrafish embryo

Jeong, Jin Young; Cho, Hyun Ju; Choi, Mi Jin; Lee, Wang Sik; Chung, Bong Hyun; Lee, Jeong Soo

doi:10.1016/j.carbon.2015.05.024

Cited by 62 publications

(35 citation statements)

References 30 publications

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“…38,39 However, polyvinyl pyrrolidone-coated GO and nano-GO coated with polyethylene glycol triggered weaker apoptosis compared to GO in a dose-dependent manner. 40,41 GBMs-induced apoptosis somehow promoted diseases. The cell cycle arrest and DNA fragmentation caused by GO could induce germline apoptosis, affecting gonad development at the concentration of 10 mg/L.…”

Section: Gbms Induce Apoptosis In Cellsmentioning

confidence: 99%

The mechanisms of graphene-based materials-induced programmed cell death: a review of apoptosis, autophagy, and programmed necrosis

Lin

Song

et al. 2017

IJN

159

108

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Graphene-based materials (GBMs) are widely used in many fields, including biomedicine. To date, much attention had been paid to the potential unexpected toxic effects of GBMs. Here, we review the recent literature regarding the impact of GBMs on programmed cell death (PCD). Apoptosis, autophagy, and programmed necrosis are three major PCDs. Mechanistic studies demonstrated that the mitochondrial pathways and MAPKs (JNK, ERK, and p38)- and TGF-β-related signaling pathways are implicated in GBMs-induced apoptosis. Autophagy, unlike apoptosis and necroptosis which are already clear cell death types, plays a vital pro-survival role in cell homeostasis, so its role in cell death should be carefully considered. However, GBMs always induce unrestrained autophagy accelerating cell death. GBMs trigger autophagy through inducing autophagosome accumulation and lysosome impairment. Mitochondrial dysfunction, ER stress, TLRs signaling pathways, and p38 MAPK and NF-κB pathways participate in GBMs-induced autophagy. Programmed necrosis can be activated by RIP kinases, PARP, and TLR-4 signaling in macrophages after GBMs exposure. Though apoptosis, autophagy, and necroptosis are distinguished by some characteristics, their numerous signaling pathways comprise an interconnected network and correlate with each other, such as the TLRs, p53 signaling pathways, and the Beclin-1 and Bcl-2 interaction. A better understanding of the mechanisms of PCD induced by GBMs may allow for a thorough study of the toxicology of GBMs and a more precise determination of the consequences of human exposure to GBMs. These determinations will also benefit safety assessments of the biomedical and therapeutic applications of GBMs.

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Section: Gbms Induce Apoptosis In Cellsmentioning

confidence: 99%

The mechanisms of graphene-based materials-induced programmed cell death: a review of apoptosis, autophagy, and programmed necrosis

Lin

Song

et al. 2017

IJN

159

108

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“…It is inevitable that graphene will be released into the environment during the production and usage of graphene-enabled consumer products, but the potential risks of graphene in the environment are not yet well understood [4]. To date, the majority of studies have focused on the toxicity of graphene [5–9], with only a limited number of studies on bioaccumulation by ecological receptors [4, 10], a particularly important component of risk assessment. In two bioaccumulation studies conducted using Daphnia magna and few layer graphene (FLG), body burdens up to 1% were recently measured after exposure for 24 h to 250 μg FLG/L [4], while graphene uptake was substantially lower for FLG partly degraded by the Fenton reaction [11].…”

Section: Introductionmentioning

confidence: 99%

“…In two bioaccumulation studies conducted using Daphnia magna and few layer graphene (FLG), body burdens up to 1% were recently measured after exposure for 24 h to 250 μg FLG/L [4], while graphene uptake was substantially lower for FLG partly degraded by the Fenton reaction [11]. In addition, the biodistribution of graphene oxide injected into zebrafish embryos has recently been studied [10]. However, no bioaccumulation studies have been conducted with graphene to our knowledge in any other ecologically relevant species.…”

Section: Introductionmentioning

confidence: 99%

Exposure of few layer graphene to Limnodrilus hoffmeisteri modifies the graphene and changes its bioaccumulation by other organisms

Mao

Liu

Lü

et al. 2016

Carbon

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While graphene has substantial commercial promise, numerous aspects regarding its ecological effects such as its potential for bioaccumulation are not well known. 14C-labeled few layer graphene (FLG) was dispersed in artificial freshwater and uptake of FLG by Limnodrilus hoffmeisteri, an oligochaete, was assessed. After exposure for 36 h to a 1 mg/L FLG suspension, the FLG body burden in the organism was nearly 60 ng/mg (on a dry mass basis). Multiple characterization results confirmed that the proteins secreted by the organisms during the exposure period coated the FLG, thus increasing its stability and decreasing its size in suspension. Uptake behaviors of Eisenia foetida exposed to FLG and protein-coated FLG at concentrations of approximately 1 mg/kg or to Daphnia magna at 100 μg/L were also quantified. Protein-coated FLG demonstrated different bioaccumulation behaviors for both organisms compared to uncoated FLG, with the FLG body burden in E. foetida increased but that in D. magna reduced. The data provide the first evidence that the proteins secreted by Limnodrilus hoffmeisteri after exposure to FLG can coat FLG, thus increasing the aqueous stability of FLG, decreasing its size, and changing its bioaccumulation potential.

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“…There have been several reports in the literature demonstrating the toxicity of different CNPs in various biological embryo models, such as chicken, [16][17][18][19] zebrafish, [20][21][22][23][24][25] and Xenopus laevis. 26,27 For example, carbon nanotubes inhibited angiogenesis of the chorioallantoic membrane in chicken embryos and caused death before day 12 of the incubation period.…”

Section: Introductionmentioning

confidence: 99%

“…21 In the case of GO, dose-dependent gross morphological defects, hatching delays, and death of zebrafish embryos were observed. 20,23,24 In in vivo experiments with X. laevis embryos, ND was highly embryotoxic and teratogenic. 27 Nevertheless, the investigations described only focused on particular nanomaterials without comparing the biological effects of different carbon allotropes manufactured by different methods and with diverse physicochemical properties.…”

Section: Introductionmentioning

confidence: 99%

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

Kurantowicz

Sawosz

Halik

et al. 2017

IJN

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In the present study, the toxicity of six different types of carbon nanoparticles (CNPs) was investigated using a chicken-embryo model. Fertilized chicken eggs were divided into the following treatment groups: placebo, diamond NPs, graphite NPs, pristine graphene, small graphene oxide, large graphene oxide, and reduced graphene oxide. Experimental solutions at a concentration of 500 μg/mL were administrated into the egg albumin. Gross pathology and the rate of survival were examined after 5, 10, 15, and 20 days of incubation. After 20 days of incubation, blood samples were collected and the weight of the body and organs measured. The relative ratio of embryo survival decreased after treatment all treatments except diamond NPs. There was no correlation between the rate of survival and the ζ-potential or the surface charge of the CNPs in solution. Body and organ weight, red blood-cell morphology, blood serum biochemical parameters, and oxidative damage in the liver did not differ among the groups. These results indicate that CNPs can remain in blood circulation without any major side effects, suggesting their potential applicability as vehicles for drug delivery or active compounds per se. However, there is a need for further investigation of their properties, which vary depending on production methods and surface functionalization.

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In vivo toxicity assessment of angiogenesis and the live distribution of nano-graphene oxide and its PEGylated derivatives using the developing zebrafish embryo

Cited by 62 publications

References 30 publications

The mechanisms of graphene-based materials-induced programmed cell death: a review of apoptosis, autophagy, and programmed necrosis

The mechanisms of graphene-based materials-induced programmed cell death: a review of apoptosis, autophagy, and programmed necrosis

Exposure of few layer graphene to Limnodrilus hoffmeisteri modifies the graphene and changes its bioaccumulation by other organisms

Toxicity studies of six types of carbon nanoparticles in a chicken-embryo model

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