In Vivo Tissue Response of Endodontic Bio-ceramic Materials

Abstract

In order to evaluate the biocompatibility and mineral repair capacity, mineral trioxide aggregate (MTA), portland cement normal setting (PCn) and fast setting (PCf) and calcium hydroxide-based paste (Calen) filled silicone tube were implanted into the dorsal subcutaneous connective tissues of 25 Wistar rats. Animals were euthanized at 24, 72 hours, 7, 15 and 30 days. Implants with surrounding tissues were fixed with 10% buffer formaldehyde and processed for histological routine techniques. Slides (5 µm serial cuts) were stained with H&E and Von Kossa stains for morphological, qualitative and quantitative analysis by light microscopy. Calen showed severe and moderate inflammatory response and granulomatous reaction with psammoma body-like formation. PCn and MTA have similar behavior, with mild inflammatory reaction from 8% and 4%, respectively. Even though, PCn and MTA demonstrated analogous biological reaction, MTA developing thick artificial mineral precipitation (p = 0,007). All sealers demonstrated a similar inflammatory response at all time periods studied (p = 0.678).

Comparative Histological and Immuno-histochemical Analysis of the Biocompatibility of Three Root Canal Sealers: An In Vivo Study

“…Dental materials must be biocompatible to avoid damaging the surrounding tissues, especially when toxic components are unintentionally extruded into the periradicular tissues. Nearly all endodontic sealers are toxic when they are first mixed; as a result, these sealers should be examined in ways that disclose their safety profile in real-world clinical settings (23) . A biomaterial's biocompatibility is evaluated by looking at how long and how intense the inflammatory reaction is.…”