In vivo targeting of acoustically reflective liposomes for intravascular and transvascular ultrasonic enhancement

Demos, Sasha M.; Alkan-Önyüksel, Hayat; Kane, Bonnie J.; Ramani, Kishin; Nagaraj, Ashwin; Greene, R. R.; Klegerman, Melvin E.; McPherson, David D.

doi:10.1016/s0735-1097(98)00607-x

Cited by 213 publications

(137 citation statements)

References 21 publications

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“…We have demonstrated specific highlighting of atheroma with intraarterial injection of anti-intercellular adhesion molecule-1-conjugated ELIP [15], as well as targeted highlighting of a left ventricular thrombus with intravenous injection of anti-fibrinogen-conjugated ELIP [16]. We have also reported entrapment of an antibiotic and of reporter genes into these ELIP with demonstration of microbial growth inhibition and gene transfection, respectively, in cultured cells [17,18].…”

Section: Discussionmentioning

confidence: 84%

“…We have previously reported development of echogenic liposomes (ELIP) for the targeted highlighting of structures [15,16] with potential for drug and gene delivery [17][18][19]. This study aimed to explore the potential of ELIP for thrombolytic loading, and to evaluate the effect of directed ultrasound exposure of the thrombolytic-loaded ELIP on effecting drug release and promoting thrombolysis.…”

mentioning

confidence: 99%

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Ultrasound-facilitated thrombolysis using tissue-plasminogen activator-loaded echogenic liposomes

Tiukinhoy-Laing¹,

Huang²,

Klegerman³

et al. 2007

Thrombosis Research

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122

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Introduction-Targeted delivery of thrombolytics to the site of occlusion is an attractive concept, with implications for the treatment of many thrombo-occlusive diseases. Ultrasound enhances thrombolysis, which can be augmented by the addition of a contrast agent. We have previously reported development of echogenic liposomes (ELIP) for targeted highlighting of structures with potential for drug and gene delivery. This study evaluated the potential of ELIP for thrombolytic loading, and the effect of ultrasound exposure of thrombolytic-loaded ELIP on thrombolytic efficacy.

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

Ultrasound-facilitated thrombolysis using tissue-plasminogen activator-loaded echogenic liposomes

Tiukinhoy-Laing¹,

Huang²,

Klegerman³

et al. 2007

Thrombosis Research

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122

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“…Moreover, targeting of the liposomes themselves is possible. We previously showed that echogenic liposomes conjugated to anti-ICAM-1 can be seen accumulating at early atheroma by ultrasound imaging, (Demos et al 1997;Demos et al 1999) In addition to releasing liposomal contents and providing an image of the process, ultrasound itself can have a therapeutically beneficial influence, namely facilitating access of the drug to its target through cavitation effects. For example, Porter et al found sitespecific drug delivery can be achieved by destroying drug-filled, contrast microbubbles in the target area with high-intensity US (Porter et al 1996)..…”

Section: Discussionmentioning

confidence: 99%

A Method to Co-Encapsulate Gas and Drugs in Liposomes for Ultrasound-Controlled Drug Delivery

Huang

McPherson

MacDonald

2008

Ultrasound in Medicine & Biology

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A novel method for the facile production of gas-containing liposomes with simultaneous drug encapsulation is described. Liposomes of phospholipid and cholesterol were prepared by conventional procedures of hydrating the lipid film, sonicating, freezing and thawing. A single, but critical modification of this procedure generates liposomes that contain gas (air, perfluorocarbon, argon); after sonication, the lipid is placed under pressure with the gas of interest. After equilibration, the sample is frozen. The pressure is then reduced to atmospheric and the suspension thawed. This procedure leads to entrapment of air in amounts up to 10% by volume by lipid dispersions at moderate (10mg/ml) concentrations of lipids. The amount of gas encapsulated increases with gas pressure and lipid concentration. Utilizing 0.32 M mannitol to provide an aqueous phase with physiological osmolarity, 1, 3, 6 or 9 atm of pressure was applied to 4 mg of lipid. This led to encapsulation of 10, 15, 20, and 30 l of gas in a total of 400 l of liposome dispersion (10mg lipids/ml), respectively. The mechanism for gas encapsulation presumably depends upon the fact that air (predominantly nitrogen and oxygen), like most solutes, dissolves poorly in ice and is excluded from the ice that forms during freezing. The excluded air then comes out of solution as air pockets that are stabilized in some form by a lipid coating. The presence of air in these preparations sensitizes them to ultrasound (1MHz, 8 W/cm 2 ,10 second) such that up to half of their aqueous contents (which could be a water soluble drug) can be released by short (10 s) applications of ultrasound. Both diagnostic and therapeutic applications of the method are conceivable.

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“…The iron oxide nanoparticles with a payload of imaging contrast agent or a drug or a combination of both, can be used for drug delivery and monitoring by images [1][2][3][4][5][6][7][8][9][10][11][12]. The iron-oxide nanoparticles bound with specific antibody also serve as a targeting system to locate the leached out antigen from the site of myocardial infarction.…”

Section: Targeted Therapeutics and Molecular Imagingmentioning

confidence: 99%

“…. The contrast mechanism of nanoparticles (liposomes or emulsions) [6][7][8], dendrimers [9,10], viral constructs [11], buckeyballs [17], or various polymers [18,19] can hold paramagnetic or superparamagnetic metals, optically active compounds (e.g., fluorescent molecules), or radionuclides. These can be detected with standard imaging equipment i.e.…”

Section: Possible Imaging Applicationsmentioning

confidence: 99%

New applications of nanoparticles in cardiovascular imaging

Sharma

Kwon

2007

Journal of Experimental Nanoscience

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Nanotechnology involves working at the atomic, molecular, and macromolecular levels including imaging. Recently, four areas have emerged in cardiovascular imaging: 1. Targeted therapeutics to deliver cardioprotective drugs at the target sites they are needed; 2. Myocardial tissue engineering to replace the defective valves, damaged heart muscle, clogged blood vessels and myocardium; 3. Molecular imaging using ''smart'' imaging agents in targeted therapeutics and imaging; 4. Biosensors and myocardial diagnostics. Several approaches to nanoparticles i.e. dendrimers, liposomes, polymer delivery molecules, cantilevers, nanoscaffolds, nanofibers are potential candidates in cardiac visualization. The extracellular matrix plays a significant role by chemokines, cytokines and growth factors. The limitations of these emerging techniques and a new possibility of MRI visualization of mouse cardiac atheroma by superparamagnetic iron-oxide gadolinium-apoferritin (SPIOA) and myoglobin (SPIOM), for targeted functional and molecular imaging of atherosclerosis are highlighted in this paper. These emerging techniques provide an opportunity for tracking functional and structural changes in myocardium and heart tissue.

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In vivo targeting of acoustically reflective liposomes for intravascular and transvascular ultrasonic enhancement

Abstract: Our data demonstrate that this novel acoustic agent can provide varying targeting with different antibodies with retention of intravascular and transvascular acoustic properties.

Cited by 213 publications

References 21 publications

Ultrasound-facilitated thrombolysis using tissue-plasminogen activator-loaded echogenic liposomes

Ultrasound-facilitated thrombolysis using tissue-plasminogen activator-loaded echogenic liposomes

A Method to Co-Encapsulate Gas and Drugs in Liposomes for Ultrasound-Controlled Drug Delivery

New applications of nanoparticles in cardiovascular imaging

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