In Vivo Tactile Stimulation-Evoked Responses in Caenorhabditis elegans Amphid Sheath Glia

Ding, Gang; Zou, Wenjuan; Zhang, Hu; Xue, Yi; Cai, Yuyan; Huang, Gui‐Fang; Chen, Lufeng; Duan, Shumin; Kang, Lijun

doi:10.1371/journal.pone.0117114

Cited by 16 publications

(31 citation statements)

References 31 publications

(90 reference statements)

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“…These findings imply that ASH is a primary mechanoreceptor neuron and that release of calcium from ITR-1-dependent intracellular stores contributes to compression-evoked calcium transients. Mechanical stimuli similar to those that activate the ASH neurons also evoke large inward currents and calcium transients in the amphid sheath cells (Ding et al 2015). These observations raise the possibility that ASH signals downstream of glia or that ASH mechanoresponses involve signals from both non-neuronal cells (glia) and sensory neurons.…”

Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

“…As shown schematically in Figure 3, electrophysiological recordings from ASH reveal robust mechanoreceptor potentials and sodium-dependent, amiloride-sensitive mechanoreceptor currents (Geffeney et al 2011; Ding et al 2015). As found in the touch receptor neurons (O’Hagan et al 2005) and CEP neurons (Kang et al 2010), MRCs activate with a millisecond latency and occur in response to both the onset and removal of mechanical deformations of the nose (Geffeney et al 2011).…”

Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

“…Although electrophysiological recordings have not been reported for any other sensory neuron linked to harsh touch sensation in C. elegans , recordings from the amphid sheath cells reveal large, mechanically activated inward currents. These currents reverse polarity near 0 mV and are not sensitive to amiloride (Ding et al 2015)—properties that make it unlikely that they are carried by a DEG/ENaC/ASIC channel. The protein partners that make up this channel are not currently known, nor is it known how mechanosensitivity in these cells contributes to harsh touch sensation in particular or to other functions more broadly.…”

Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

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How Caenorhabditis elegans Senses Mechanical Stress, Temperature, and Other Physical Stimuli

2019

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Caenorhabditis elegans lives in a complex habitat in which they routinely experience large fluctuations in temperature, and encounter physical obstacles that vary in size and composition. Their habitat is shared by other nematodes, by beneficial and harmful bacteria, and nematode-trapping fungi. Not surprisingly, these nematodes can detect and discriminate among diverse environmental cues, and exhibit sensory-evoked behaviors that are readily quantifiable in the laboratory at high resolution. Their ability to perform these behaviors depends on <100 sensory neurons, and this compact sensory nervous system together with powerful molecular genetic tools has allowed individual neuron types to be linked to specific sensory responses. Here, we describe the sensory neurons and molecules that enable C. elegans to sense and respond to physical stimuli. We focus primarily on the pathways that allow sensation of mechanical and thermal stimuli, and briefly consider this animal’s ability to sense magnetic and electrical fields, light, and relative humidity. As the study of sensory transduction is critically dependent upon the techniques for stimulus delivery, we also include a section on appropriate laboratory methods for such studies. This chapter summarizes current knowledge about the sensitivity and response dynamics of individual classes of C. elegans mechano- and thermosensory neurons from in vivo calcium imaging and whole-cell patch-clamp electrophysiology studies. We also describe the roles of conserved molecules and signaling pathways in mediating the remarkably sensitive responses of these nematodes to mechanical and thermal cues. These studies have shown that the protein partners that form mechanotransduction channels are drawn from multiple superfamilies of ion channel proteins, and that signal transduction pathways responsible for temperature sensing in C. elegans share many features with those responsible for phototransduction in vertebrates.

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Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

Section: Mechanosensation and Sensory Mechanotransductionmentioning

confidence: 99%

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How Caenorhabditis elegans Senses Mechanical Stress, Temperature, and Other Physical Stimuli

2019

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“…This is especially crucial during the screening of a random single-FP biosensor library, where quantitation is essential to correct for expression artifacts [7][8][9][10] . This drawback is commonly addressed by co-expression or terminal fusion of a spectrally distinct FP, such as mCherry, as a normalization control [7][8][9][10][11][12] . However, co-expression of the reference is not a reliably accurate control, as for example, variation in expression or protein levels can lead to artifacts of the signal readout.…”

Section: Introductionmentioning

confidence: 99%

Matryoshka: Ratiometric biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

Oltrogge

et al. 2017

Preprint

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Sensitivity, dynamic and detection range as well as exclusion of expression and instrumental artifacts are critical for the quantitation of data obtained with fluorescent protein (FP)-based biosensors in vivo. Current biosensors designs are, in general, unable to simultaneously meet all these criteria. Here, we describe a generalizable platform to create dual-FP biosensors with large dynamic ranges by employing a single FP-cassette, named GO-(Green-Orange) Matryoshka. The cassette nests a stable reference FP (large Stokes shift LSSmOrange) within a reporter FP (circularly permuted green FP). GO-Matryoshka yields green and orange fluorescence upon blue excitation. As proof of concept, we converted existing, single-emission biosensors into a series of ratiometric calcium sensors (MatryoshCaMP6s) and ammonium transport activity sensors (AmTryoshka1;3). We additionally identified the internal acid-base equilibrium as a key determinant of the GCaMP dynamic range. Matryoshka technology promises flexibility in the design of a wide spectrum of ratiometric biosensors and expanded in vivo applications.

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“…S1B) and ADE and PDE socket glia in the body. Table 1 AMsh or PHsh F16F9.3 2 kb promoter (Bacaj et al, 2008;Braunreiter et al, 2014;Heiman and Shaham, 2009;Low et al, 2019;Mizeracka et al, 2019;Oikonomou et al, 2011;Procko et al, 2012Procko et al, , 2011Wallace et al, 2016;Yip and Heiman, 2018) vap-1 (developmentally regulated) 2797 bp or 5205 bp promoter (Bacaj et al, 2008;Ding et al, 2015;Oikonomou et al, 2011;Perens and Shaham, 2005;Procko et al, 2011;Wallace et al, 2016;Wang et al, 2017Wang et al, , 2012Wang et al, , 2008 2.5 kb promoter (Bacaj et al, 2008;Grant et al, 2015;Mizeracka et al, 2019;Oikonomou et al, 2012Oikonomou et al, , 2011Procko et al, 2011;Wang et al, 2017Wang et al, , 2008 fig-1 2.2 kb promoter (Bacaj et al, 2008;Fenk and de Bono, 2015;Kage-Nakadai et al, 2016;Wallace et al, 2016;Yoshida et al, 2016) ver-1 (thermo-and dauerregulated) 2110 bp promoter (Popovici et al, 2002;Procko et al, 2012Procko et al, , 2011Yoshida et al, 2016)...…”

Section: Single-cell Transcriptional Profiling Identifies New Cell-tymentioning

confidence: 99%

Cell-type-specific promoters forC. elegansglia

Fung

Wexler

Heiman

2020

Preprint

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Cell-type-specific promoters for C. elegans glia 2 ABSTRACT Glia shape the development and function of the C. elegans nervous system, especially its sense organs and central neuropil (nerve ring). Cell-type-specific promoters allow investigators to label or manipulate individual glial cell types, and therefore provide a key tool for deciphering glial function. In this technical resource, we compare the specificity, brightness, and consistency of cell-type-specific promoters for C. elegans glia. We identify a set of promoters for the study of seven glial cell types (F16F9.3, amphid and phasmid sheath glia; F11C7.2, amphid sheath glia only; grl-2, amphid and phasmid socket glia; hlh-17, cephalic (CEP) sheath glia; and grl-18, inner labial (IL) socket glia) as well as a pan-glial promoter (mir-228). We compare these promoters to promoters that are expressed more variably in combinations of glial cell types (delm-1 and itx-1). We note that the expression of some promoters depends on external conditions or the internal state of the organism, such as developmental stage, suggesting glial plasticity. Finally, we demonstrate an approach for prospectively identifying cell-type-specific glial promoters using existing single-cell sequencing data, and we use this approach to identify two novel promoters specific to IL socket glia (col-53 and col-177).

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In Vivo Tactile Stimulation-Evoked Responses in Caenorhabditis elegans Amphid Sheath Glia

Cited by 16 publications

References 31 publications

How Caenorhabditis elegans Senses Mechanical Stress, Temperature, and Other Physical Stimuli

How Caenorhabditis elegans Senses Mechanical Stress, Temperature, and Other Physical Stimuli

Matryoshka: Ratiometric biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

Cell-type-specific promoters forC. elegansglia

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