In vivo study of anti‐epidermal growth factor receptor antibody‐based iron oxide nanoparticles (anti‐EGFR‐SPIONs) as a novel MR imaging contrast agent for lung cancer (LLC1) cells detection

Abstract

Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with anti-epidermal growth factor receptor monoclonal antibody (anti-EGFR-SPIONs) were characterised, and its cytotoxicity effects, ex vivo and in vivo studies on Lewis lung carcinoma (LLC1) cells in C57BL/6 mice were investigated. The broadband at 679.96 cm −1 relates to Fe-O, which verified the formation of the anti-EGFR-Mab with SPIONs was obtained by the FTIR. The TEM images showed spherical shape 20 and 80 nm-sized for nanoparticles and the anti-EGFR-SPIONs, respectively. Results of cell viability at 24 h after incubation with different concentrations of nanoprobe showed it has only a 20% reduction in cell viabilities. The synthesised nanoprobe administered by systemic injection into C57BL/6 mice showed good Fe tumour uptake and satisfied image signal intensity under ex vivo and in vivo conditions. A higher concentration of nanoprobe was achieved compared to non-specific and control, indicating selective delivery of nanoprobe to the tumour. It is concluded that the anti-EGFR-SPIONs was found to be as an MR imaging contrast nanoagent for lung cancer (LLC1) cells detection. 2 Materials and methods Nanomag ®-D-SPIONs 20 nm nanoparticles in suspension (surface PEG-COOH, product code: 79-56-201) were provided from micromod Partikeltechnologie GmbH (Rostock, Germany). All chemicals were purchased from Sigma Chemical Co.

Atranorin driven by nano materials SPION lead to ferroptosis of gastric cancer stem cells by weakening the mRNA 5-hydroxymethylcytidine modification of the Xc-/GPX4 axis and its expression

“…Our previous studies have shown that SPION can efficiently bind microRNAs or siRNAs, mediate their transfection into in cells and nematodes, inhibit the proliferation and invasion of tumor cells, or promote the directional differentiation of stem cells [19,[22][23][24][25][26]. At the same time, we and other research groups also found that the distribution of tumor cells containing SPION in nude mice can be easily tracked by nuclear magnetic resonance (NMR) detection [25,27,28]. In addition, there are many reports that SPION can combine with a variety of chemotherapeutic drugs, greatly improving the penetration, killing, and persistence of chemotherapeutic drugs in tumor cells [29,30].…”

Enhancing the therapeutic efficacy of nanoparticles for cancer treatment using versatile targeted strategies

“…Gupta and colleagues constructed a Gem encapsulated nanoplatform against pancreatic cancer through covalent binding to EGFR antibodies [ 37 ], presenting higher cytotoxicity of the designed nanoplatform for EGFR-overexpressing pancreatic cell lines. Moreover, anti-EGFR functionalized Fe 2 O 3 nanoparticles can be used as magnetic resonance imaging contrast agents for tumor diagnosis [ 38 , 39 ]. In an interesting review article, Yi and colleagues discussed the role of EGFR tyrosine kinase inhibitors in targeted nanoplatforms for tumor treatment [ 40 ].…”

Nanotechnology Advances in the Detection and Treatment of Cancer: An Overview

“…Even so, with some experiments suggesting monoclonal antibody-conjugated SPIONs have a high affinity to antigen-expressing tissues, this has proved not to be the case [212]. In laboratory animals, EGFR antibodies were integrated with SPIONs to detect small lung cells, esophageal squamous cells, and colorectal carcinomas [213][214][215][216][217]. However, it dramatically enhanced the scale of the nanoparticle-antibody conjugation system, and a decrease in stealth-like functionality was observed.…”