In vivo studies on chemically induced aneuploidy in mouse somatic and germinal cells

Leopardi, Paola; Zijno, Andrea; Bassani, B.; Pacchierotti, Francesca

doi:10.1016/0027-5107(93)90151-5

Cited by 52 publications

(18 citation statements)

References 38 publications

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“…These 7 chemicals also increased hyperploidy in second meiotic divisions of mouse spermatocytes in the same laboratory (23). The other laboratory obtained an increase in hyperploid second meiotic divisions with COL, HQ, and TB, but not with CH, DZ, and CD (14). The differences in results from the two laboratories are most likely due to different test protocols.…”

Section: Results Of In Vivo Testingmentioning

confidence: 87%

“…One laboratory also obtained a positive micronucleus response with TB and PY (14). Another laboratory (not shown in Table 2) obtained a weak micronucleus induction with CH, DZ, TB, CD, TM, and PY, which was only observed with one of several doses in the lower dose range tested and never exceeded a doubling of the concurrent control values; however, the positive effect was confirmed in a second experiment (18).…”

Section: Results Of In Vivo Testingmentioning

confidence: 90%

“…Chromosome counts in BrdUlabeled second mitoses ofmouse bone marrow cells showed hyperploidy to be induced by COL, CH, HQ, and VBL (14)(15)(16)(17). The other participating laboratories all found a small increase in hyperploid cells with these four chemicals and additionally with EZ and DZ (18).…”

Section: Results Of In Vivo Testingmentioning

confidence: 99%

“…Micronuclei were found in mouse polychromatic erythrocytes after treatment with COL, HQ, and VBL by all participating laboratories (14,16,18,20). One laboratory also obtained a positive micronucleus response with TB and PY (14).…”

Section: Results Of In Vivo Testingmentioning

confidence: 99%

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Development of screening tests for aneuploidy induction by environmental pollutants.

Adler¹,

Parry²

1993

Environ Health Perspect

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When legally required mutagenicity testing of chemicals is undertaken, the important genetic end point of aneuploidy is not included because validated test methods are lacking. Therefore, the Commission of the European Communities (CEC) has funded a research program to develop and validate tests for aneuploidy induction. Ten chemicals, selected on the basis of their ability to interact with cell organelles relevant for aneuploidy induction, were tested in 11 laboratories. The assays ranged from in vitro tubulin assembly studies to in vivo germ-cell tests. The results allow several conclusions: a) Fungal aneuploidy tests are not capable of detecting inhibitors of mammalian tubulin polymerization such as colchicine and vinblastine. Therefore, they will not play a role in screening for aneuploidy but are of value for studying the relationship between induced aneuploidy and recombination. b) Chemicals that induce aneuploidy in mammalian germ cells are readily detected in the in vitro mammalian cell systems. Some chemicals such as thiabendazole and thimerosal induce aneuploidy in vitro but do not appear to be very effective in vivo. c) Cell division aberrations induced in mammalian cells in vitro seem to be predictive for aneuploidy induction in the same cell type. Likewise, c-mitotic effects and cell cycle delay in vivo in mitotic and meiotic cells correlate with aneuploidy induction in the respective tissue. A second CEC Aneuploidy Program has started recently to refine the most promising test protocols, to provide understanding of variety of mechanisms by which chemicals induce aneuploidy, and to establish a data base for aneugens among environmental pollutants.

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Section: Results Of In Vivo Testingmentioning

confidence: 87%

Section: Results Of In Vivo Testingmentioning

confidence: 90%

Section: Results Of In Vivo Testingmentioning

confidence: 99%

Section: Results Of In Vivo Testingmentioning

confidence: 99%

See 2 more Smart Citations

Development of screening tests for aneuploidy induction by environmental pollutants.

Adler¹,

Parry²

1993

Environ Health Perspect

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“…Most of the clinically detected aneuploidy events in humans arise as a result of non-disjunction of oocytes at Met I Jacobs and Hassold, 1980 . Furthermore, considerably higher levels of aneuploidy are induced in oocytes than in spermatocytes by vinblastine and colchicine Russo and Pacchierotti, 1988;Mailhes et al, 1990;Miller and Adler, 1992;Leopardi et al, 1993 . Thus, in vitro maturation of mouse oocyete demonstrated in this study is a useful model for the detection of chemically induced aneuploidy and for the detection of stage-specific effects of chemicals during meiosis.…”

mentioning

confidence: 99%

Aneugenic effects of carbendazim and griseofulvin as assayed in the in vitro maturation system of mouse oocytes

Tanaka

Sasanami

Toriyama

et al. 2004

Environ. Mutagen Res.

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To evaluate the aneugenic effects of carbendazim MBC and griseofulvin GF on meiosis, we cultured mouse oocytes, allowing them to mature in vitro in the presence of MBC and GF. The incidence of hyperploidy was significantly increased in oocytes cultured with 0.6 µg/mL or higher concentration of MBC, while the incidence of diploidy was significantly increased in oocytes cultured with 1 or 3 µg/mL of GF. To investigate the stage-specific effects of these chemicals during meiotic progression, the oocytes were exposed to chemicals for the initial 7 h or final 8 h of the culture. Both MBC and GF were found to effectively induce numerical aberration during the final half of the culture. Thus, in vitro maturation of mouse oocytes is a useful model for the detection of chemically induced aneuploidy and for the detection of stage-specific effects of chemicals during meiosis.

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Effects of carbendazim (methyl 2-benzimidazole carbamate; MBC) on meiotic spermatocytes and subsequent spermiogenesis in the rat testis

Nakai

Hess

1997

Anat. Rec.

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In vivo studies on chemically induced aneuploidy in mouse somatic and germinal cells

Cited by 52 publications

References 38 publications

Development of screening tests for aneuploidy induction by environmental pollutants.

Development of screening tests for aneuploidy induction by environmental pollutants.

Aneugenic effects of carbendazim and griseofulvin as assayed in the in vitro maturation system of mouse oocytes

Effects of carbendazim (methyl 2-benzimidazole carbamate; MBC) on meiotic spermatocytes and subsequent spermiogenesis in the rat testis

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