In vivo studies on bioavailability, toxicity, and antioxidant defense of organic selenium-enriched microalga biomass in Wistar rats

Ragini, Reshma; Arumugam, Muthu

doi:10.1007/s10811-023-03007-x

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…Inorganic selenium forms (primarily selenite and selenate) can be biotransformed into organic forms (selenomethionine, selenocysteine, and other seleniocompounds) through incorporation into amino acids or polysaccharides ( Qian et al, 2023 ). These organic selenium compounds present higher bioaccessibility, reduced toxicity, and more beneficial effects than inorganic forms ( Ragini & Arumugam, 2023 ; Shao et al, 2021 ). Given selenium and selenoproteins’ high nutritional value, selenium biofortification using agricultural products grown in bioaccumulative organisms is a promising alternative ( Ávila et al, 2013 ; Businelli et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

Du,

Huang,

Huang

et al. 2024

PeerJ

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Although Morchella esculenta (L.) Pers. is an edible and nutritious mushroom with significant selenium (Se)-enriched potential, its biological response to selenium stimuli remains unclear. This study explored the effect of selenium on mushroom growth and the global gene expression profiles of M. esculenta. While 5 µg mL−1selenite treatment slightly promoted mycelia growth and mushroom yield, 10 µg mL−1significantly inhibited growth. Based on comparative transcriptome analysis, samples treated with 5 µg mL−1 and 10 µg mL−1 of Se contained 16,061 (452 upregulated and 15,609 downregulated) and 14,155 differentially expressed genes (DEGs; 800 upregulated and 13,355 downregulated), respectively. Moreover, DEGs were mainly enriched in the cell cycle, meiosis, aminoacyl-tRNA biosynthesis, spliceosome, protein processing in endoplasmic reticulum pathway, and mRNA surveillance pathway in both selenium-treated groups. Among these, MFS substrate transporter and aspartate aminotransferase genes potentially involved in Se metabolism and those linked to redox homeostasis were significantly upregulated, while genes involved in isoflavone biosynthesis and flavonoid metabolism were significantly downregulated. Gene expression levels increased alongside selenite treatment concentration, suggesting that high Se concentrations promoted M. esculenta detoxification. These results can be used to thoroughly explain the potential detoxification and Se enrichment processes in M. esculenta and edible fungi.

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Section: Introductionmentioning

confidence: 99%

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

Du,

Huang,

Huang

et al. 2024

PeerJ

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“…It protects organisms from radiation damage through mechanisms such as clearing free radicals, maintaining glutathione levels, and regulating signaling pathways ( Shimazu et al, 1964 ). However, the bioavailability and activity of selenium are influenced by factors such as its chemical form, dosage, and distribution within the organism ( Ragini and Arumugam, 2023 ). For example, In the form of organic selenium compounds, selenium exhibits radiation protection effects only at moderate concentrations, while higher concentrations are toxic to cells ( Ragini and Arumugam, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, the bioavailability and activity of selenium are influenced by factors such as its chemical form, dosage, and distribution within the organism ( Ragini and Arumugam, 2023 ). For example, In the form of organic selenium compounds, selenium exhibits radiation protection effects only at moderate concentrations, while higher concentrations are toxic to cells ( Ragini and Arumugam, 2023 ). Moreover, selenium compounds demonstrate a combination of preventive (effective before exposure to ionizing radiation) and mitigating (effective after exposure to ionizing radiation, prior to the development of the first clinical signs of radiation sickness), demonstrating their multiple characteristics.…”

Section: Introductionmentioning

confidence: 99%

Radioprotective effect of polyvinylpyrrolidone modified selenium nanoparticles and its antioxidation mechanism in vitro and in vivo

Li,

Lu,

Jiang

et al. 2024

Front. Bioeng. Biotechnol.

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ObjectivePolyvinylpyrrolidone (PVP) is a commonly used biomedical polymer material with good water solubility, biocompatibility, low immunogenicity, and low toxicity. The aim of this study is to investigate the antioxidant mechanism and clinical potential of PVP modified selenium nanoparticles (PVP-Se NPs) as a new radioprotective agent.MethodsA laser particle size analyzer and transmission electron microscope were used to characterize PVP-Se nanoparticles prepared by chemical reduction. Human umbilical vein endothelial cells (HUVECs) were used to evaluate the radiation protective effects of PVP-Se NPs. SD rats were employed as an in vivo model to identify the most effective concentration of PVP-Se NPs and assess their potential radioprotective properties. Western blot (WB) was used to detect the expression of nuclear factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling proteins in human umbilical vein endothelial cells (HUVECs) and rat liver and kidney tissues.ResultsPVP-Se NPs could reduce the oxidative stress injury and inflammatory response caused by X-ray irradiation in HUVECs and rats, and inhibit cell apoptosis by modulating NF-κB and MAPK signaling pathways. PVP-Se NPs could increase HUVECs viability, reduce apoptosis, inhibit inflammatory factors IL-1β, IL-6 and TNF-α, improve the survival rate of rats, promote antioxidant enzyme activities in cells and rats, reduce malondialdehyde concentration in serum, and reduce the expression of inflammatory factors such as IL-1β, IL-6 and TNF-α in cell supernatant and liver and kidney tissues. PVP-Se NPs could significantly reduce the phosphorylation levels of NF-κB and MAPK pathway-associated proteins in HUVECs and rat liver and kidney tissues (p < 0.05).ConclusionPVP-Se NPs can protect against radiation-induced oxidative damage by modulating NF-kB and MAPK pathways, providing a theoretical basis and experimental data for their use as an effective radioprotective agent.

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“…Recently, our group decoded the selenium protein T and associated SECIS machinery from Microalgae [ 31 ]. We also developed a patented bioprocess to augment the bioconversion of organic selenium in an edible marine microalgae [ 29 ] and validated its applicability in the murine model [ 30 ]. However, organic selenium-enriched edible microalgae has wide applications in the therapeutic and feed industries.…”

Section: Introductionmentioning

confidence: 99%

In vivo studies on bioavailability, toxicity, and antioxidant defense of organic selenium-enriched microalga biomass in Wistar rats

Cited by 4 publications

References 33 publications

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

De novo assembly and characterization of the transcriptome of Morchella esculenta growth with selenium supplementation

Radioprotective effect of polyvinylpyrrolidone modified selenium nanoparticles and its antioxidation mechanism in vitro and in vivo

Green synthesis, characterization and functional validation of bio-transformed selenium nanoparticles

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