In vivo studies of transdermal nanoparticle delivery with microneedles using photoacoustic microscopy

Moothanchery, Mohesh; Seeni, Razina Z.; Xu, Chen; Pramanik, Manojit

doi:10.1364/boe.8.005483

Cited by 43 publications

(33 citation statements)

References 38 publications

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“…They suggested that the MN should be long enough to puncture through the stratum corneum and to create a bypass to transport the drug. Until now, the MN technology has been developed in order to facilitate the intradermal delivery of target substances and enhance the skin permeability for nanoparticle [30][31][32]. The recovered amount of retinal in the dermis from PRN-loaded MN application was 0.85 µg/cm 2 or 31.7% from the total amount applied, while it was 0.35 µg/cm 2 or 13.2% for the PRN-treated group and 0.22 µg/cm 2 or 8.2% for the RAL-treated group.…”

Section: Discussionmentioning

confidence: 99%

Microneedle-Facilitated Intradermal Proretinal Nanoparticle Delivery

et al. 2020

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Topical retinoid treatments stimulate biological activities in the skin. The main physical barrier, which limits the efficacy of transdermal drug delivery, is the stratum corneum. Proretinal nanoparticles (PRN) have already been proven to efficiently deliver retinal into the epidermis. In the present study, two transdermal drug delivery systems, microneedles (MN) and PRN, were combined to directly target the dermis. The microchannels induced by the MN, the PRN localization in the microchannels and the skin closure kinetics were investigated by non-invasive imaging techniques, such as dermoscopy, optical coherence tomography and multiphoton tomography. Additionally, the amount of retinal in the epidermis and dermis after application in three different forms (PRN-Loaded microneedles, PRN suspension or conventional retinal solution) was compared. All imaging techniques confirmed the formation of microchannels in the skin, which were partly still detectable after 24 h. Multiphoton tomography showed the release of PRN from the MN within the microchannels. The recovered retinal concentration in the dermis was significantly higher when applied via PRN-loaded microneedles. We hypothesized that this platform of PRN-loaded microneedles can provide a rapid and efficient administration of retinal in the dermis and could be of benefit in some skin conditions such as atrophic scar or photo-aged skin.

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Section: Discussionmentioning

confidence: 99%

Microneedle-Facilitated Intradermal Proretinal Nanoparticle Delivery

et al. 2020

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“…By combining optical contrast and US resolution, PAI offers several advantages: (i) it is noninvasive and uses nonionizing radiation, hence it can be repeatedly used in vivo by keeping the excitation energy below the safety limit; (ii) it provides label-free imaging; (iii) it is speckle-free; (iv) it has higher penetration depth, up to ∼4 cm in vivo 36 and ∼12 cm in vitro; 37 (v) it is faster and less expensive compared to MRI, PET, x-ray CT; 38 (vi) it provides multiscale imaging, allows imaging organelles to organs with consistent contrast while keeping the same depth-toresolution ratio; 38,39 (vii) it provides direct imaging of optical absorption with 100% relative sensitivity-the sensitivity of PAI is 100 times higher than that of OCT and confocal microscopy; and (viii) it provides anatomical, functional, molecular, and kinetic information. 40 PAI was successfully implemented as photoacoustic nanoscopy, [41][42][43][44][45][46] photoacoustic microscopy (PAM), [47][48][49][50][51][52] photoacoustic endoscopy, [53][54][55][56] preclinical photoacoustic computed tomography/photoacoustic tomography *Address all correspondence to Manojit Pramanik, E-mail: manojit@ntu.edu.sg (PACT/PAT), [57][58][59][60][61][62] and clinical PACT. [63][64][65][66][67][68] For deep-tissue imaging, PACT/PAT systems are suitable and hence hold better chance to find preclinical/clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging in the second near-infrared window: a review

2019

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Photoacoustic (PA) imaging is an emerging medical imaging modality that combines optical excitation and ultrasound detection. Because ultrasound scatters much less than light in biological tissues, PA generates high-resolution images at centimeters depth. In recent years, wavelengths in the second near-infrared (NIR-II) window (1000 to 1700 nm) have been increasingly explored due to its potential for preclinical and clinical applications. In contrast to the conventional PA imaging in the visible (400 to 700 nm) and the first NIR-I (700 to 1000 nm) window, PA imaging in the NIR-II window offers numerous advantages, including high spatial resolution, deeper penetration depth, reduced optical absorption, and tissue scattering. Moreover, the second window allows a fivefold higher light excitation energy density compared to the visible window for enhancing the imaging depth significantly. We highlight the importance of the second window for PA imaging and discuss the various NIR-II PA imaging systems and contrast agents with strong absorption in the NIR-II spectral region. Numerous applications of NIR-II PA imaging, including whole-body animal imaging and human imaging, are also discussed.

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“…Photoacoustic microscopy (PAM) is an emerging innovative hybrid in vivo imaging modality, which combines optical absorption contrast and ultrasonic resolution [1][2][3][4][5][6][7][8][9]. In PAM, a short pulsed laser will irradiate the sample to generate acoustic waves due to the temperature rise induced by the sample.…”

Section: Introductionmentioning

confidence: 99%

Optical resolution photoacoustic microscopy based on multimode fibers

Moothanchery

Kim

et al. 2018

Biomed. Opt. Express

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Photoacoustic microscopy (PAM) is a multiscale imaging technique. In optical-resolution photoacoustic microscopy (OR-PAM), a single mode (SM) fiber is normally used as the source of optical excitation to be focused into a diffraction-limited spot. Recent advances in OR-PAM have improved its imaging speed using microelectromechanical systems (MEMS). Here we report for the first time the use of a multimode (MM) fiber as the optical excitation source for high resolution OR-PAM imaging. A high-speed MEMS scanner based OR-PAM system combined with the mechanical movement to provide wide area imaging was used. The use of multimode fiber for achieving tight optical focus would make the optical alignment easier and high repetition rate light delivery possible for high-speed OR-PAM imaging. A lateral resolution of 3.5 µm and axial resolution of 27 µm with ~1.5 mm imaging depth was successfully demonstrated using the system. The efficacy of multimode fibers for achieving tight focus is beneficial for developing high-resolution photoacoustic endoscopy systems and can be combined with other optical endoscopic imaging modalities as well.

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In vivo studies of transdermal nanoparticle delivery with microneedles using photoacoustic microscopy

Cited by 43 publications

References 38 publications

Microneedle-Facilitated Intradermal Proretinal Nanoparticle Delivery

Microneedle-Facilitated Intradermal Proretinal Nanoparticle Delivery

Photoacoustic imaging in the second near-infrared window: a review

Optical resolution photoacoustic microscopy based on multimode fibers

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