In vivo studies of the early, peritoneal, cellular and free radical response in rats infected with Fasciola hepatica by flow cytometric analysis

Jedlina, Luiza; Kozak-Ljunggren, Monika; Wędrychowicz, H.

doi:10.1016/j.exppara.2011.02.004

Cited by 21 publications

(24 citation statements)

References 35 publications

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“…To further describe the immuno-pathological responses to F. hepatica infection and complement previous studies of the peritoneal compartment 55–57 , we employed a novel approach to identify the proteins present within sheep peritoneal fluid during early F. hepatica infection using proteomics. This was performed by electrophoretically separating the proteins under denaturing conditions and then slicing fractions above and below the predominant host proteins (circa 67 kDa albumin; Supplementary Figure 1).…”

Section: Discussionmentioning

confidence: 99%

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Ruiz-Campillo

Molina-Hernández

Escamilla

et al. 2017

Sci Rep

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Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.

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Section: Discussionmentioning

confidence: 99%

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Ruiz-Campillo

Molina-Hernández

Escamilla

et al. 2017

Sci Rep

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“…However, the cells decreased 4 days p.i. [44]. Therefore, the experimental set-up depends on the response outcomes needed.…”

Section: Discussionmentioning

confidence: 99%

The absence of MyD88 has no effect on the induction of alternatively activated macrophage during Fasciola hepatica infection

et al. 2011

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BackgroundAlternatively activated macrophages (AAMϕ) play important roles in allergies and responses to parasitic infections. However, whether signaling through toll-like receptors (TLRs) plays any role in AAMϕ induction when young Fasciola hepatica penetrates the liver capsule and migrates through the liver tissue is still unclear.ResultsThe data show that the lack of myeloid differentiation factor 88 (MyD88) has no effect on the AAMϕ derived from the bone marrow (BMMϕ) in vitro and does not impair the mRNA expression of arginase-1, resistin-like molecule (RELMα), and Ym1 in BMMϕs. The Th2 cytokine production bias in splenocytes was not significantly altered in F. hepatica-infected mice in the absence of MyD88 in vitro and in the pleural cavity lavage in vivo. In addition, MyD88-deficiency has no effect on the arginase production of the F. hepatica elicited macrophages (Fe Mϕs), production of RELMα and Ym1 proteins and mRNA expression of Ym1 and RELMα of macrophages in the peritoneal cavity 6 weeks post F. hepatica infection.ConclusionsThe absence of MyD88 has no effect on presence of AAMϕ 6 weeks post F. hepatica infection.

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“…An effective response in rats infected with F. hepatica has been associated with a significant increase in eosinophil infiltration of the gut lamina propria in early post-infection stages (Van Milligen et al, 1998. In F. hepatica infected rats, peritoneal eosinophils producing very high levels of nitric oxide (NO) have been reported (Jedlina et al, 2011). In sheep, peritoneal eosinophils and macrophages, as well as mammary gland eosinophils from F. gigantica resistant Indonesian thin-tail (ITT) sheep were able to kill juvenile F. gigantica in vitro by antibody-dependent cytotoxicity, but they did not kill larvae of F. hepatica, suggesting that eosinophils are important effector cells involved in the resistance of sheep to F. gigantica (Piedrafita et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Escamilla

Bautista

Zafra

et al. 2016

Veterinary Parasitology

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In vivo studies of the early, peritoneal, cellular and free radical response in rats infected with Fasciola hepatica by flow cytometric analysis

Cited by 21 publications

References 35 publications

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

The absence of MyD88 has no effect on the induction of alternatively activated macrophage during Fasciola hepatica infection

Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

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