In vivo Skin Penetration, Radical Protection, and Structural Changes after Topical Application of a Herbal Oil Cream Compared to Topical Calcipotriol in Mild to Moderate Psoriasis

Lademann, Juergen; Mansouri, Parvin; Nahavandi, Ali; Ahlers, Axel; Zibakalam-Mofrad, Fatemeh; Brower, Barbara; Nahavandi, Maryam; Feddern, Frank; Darvin, Maxim E.; Schanzer, Sabine; Richter, Heike; Meinke, Martina C.; Rezaii, Seyed Ahmad; Rohaninasab, Masoumeh; Farshi, Susan; Iacobelli, Massimo; Jung, Sora

doi:10.1159/000518970

Cited by 2 publications

(2 citation statements)

References 59 publications

(66 reference statements)

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“…The recent work [189] utilised EPR measurements to quantify the radical formation in vivo during the skin treatment by herbal oils as a time function, while the Raman spectroscopy gave information on the penetration depth using specific herbal compounds' spectral fingerprints. Therefore, this combined spatiotemporal information may play a role in the background for the future building of a kind of reaction-diffusion model of the process.…”

Section: Combining Epr and Raman Spectroscopy Methods And Datamentioning

confidence: 99%

Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

Postnikov,

Wasiak,

Bartoszek

et al. 2023

Molecules

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In this work, we review some physical methods of macroscopic experiments, which have been recently argued to be promising for the acquisition of valuable characteristics of biomolecular structures and interactions. The methods we focused on are electron paramagnetic resonance spectroscopy, Raman spectroscopy, and differential scanning calorimetry. They were chosen since it can be shown that they are able to provide a mutually complementary picture of the composition of cellular envelopes (with special attention paid to mycobacteria), transitions between their molecular patterning, and the response to biologically active substances (reactive oxygen species and their antagonists—antioxidants—as considered in our case study).

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Section: Combining Epr and Raman Spectroscopy Methods And Datamentioning

confidence: 99%

Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

Postnikov,

Wasiak,

Bartoszek

et al. 2023

Molecules

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“…[26][27][28] Several methods have been used to examine the psoriatic SC, like TEWL, corneometry, 'attenuated total reflectance-Fouriertransform infrared spectroscopy', 29,30 tape-stripping, 31 multiphoton CARS tomography, 32 optical coherence tomography, 33 diffuse reflectance spectroscopy, 34 scanning electron microscopy 35 and laser scanning microscopy. 36,37 Previous studies have revealed a less ordered SC structure regarding lipid organisation in psoriatic lesions, 10,36,38 which is thought to be partly due to differences in the composition of ceramides. 38,39 The FFA concentration of lesional psoriatic skin (LPS) has been found to be lower than of non-lesional psoriatic skin (nLPS).…”

Section: Introductionmentioning

confidence: 99%

Altered structure indicating reduced barrier function of lesional compared to non‐lesional psoriatic skin—A non‐invasive in vivo study of the human stratum corneum with confocal Raman micro‐spectroscopy

Zolotas,

Schleusener,

Lademann

et al. 2023

Experimental Dermatology

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Psoriasis, one of the most common skin diseases affecting roughly 2%–3% of the world population, is associated with a reduced skin barrier function (SBF) that might play an important role in its pathophysiology. The SBF is provided primarily by the stratum corneum (SC) of the skin. Previous studies have revealed a higher trans‐epidermal water loss, lower hydration, abnormal concentration and composition of intercellular lipids, as well as alterations in secondary keratin structure in the psoriatic SC. We compared on molecular level lesional psoriatic skin (LPS) with non‐lesional psoriatic skin (nLPS) from 19 patients non‐invasively in vivo, using confocal Raman micro‐spectroscopy. By analysing the corresponding Raman spectra, we determined SBF‐defining parameters of the SC depth‐dependently. Our results revealed a lower total lipid concentration, a shift of lamellar lipid organisation towards more gauche‐conformers and an increase of the less dense hexagonal lateral packing of the intercellular lipids in LPS. Furthermore, we observed lower natural moisturising factor concentration, lower total water as well as a strong tendency towards less strongly bound and more weakly bound water molecules in LPS. Finally, we detected a less stable secondary keratin structure with increased β‐sheets, in contrast to the tertiary structure, showing a higher degree of folded keratin in LPS. These findings clearly suggest structural differences indicating a reduced SBF in LPS, and are discussed in juxtaposition to preceding outcomes for psoriatic and healthy skin. Understanding the alterations of the psoriatic SC provides insights into the exact pathophysiology of psoriasis and paves the way for optimal future treatments.

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In vivo Skin Penetration, Radical Protection, and Structural Changes after Topical Application of a Herbal Oil Cream Compared to Topical Calcipotriol in Mild to Moderate Psoriasis

Cited by 2 publications

References 59 publications

Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

Altered structure indicating reduced barrier function of lesional compared to non‐lesional psoriatic skin—A non‐invasive in vivo study of the human stratum corneum with confocal Raman micro‐spectroscopy

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