In-vivo single cell protein interaction investigation using microfluidic platform

Davaji, Benyamin; Biener, Gabriel; Raicu, Valerică; Lee, C.H.

doi:10.1109/transducers.2015.7181231

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…The nickel metal film was patterned in a serpentine shape to increase the sensing area and to set resistance at room temperature around 500 Ω, which showed an optimal sensitivity in this work. The fabrication process for the RTD sensor on SiN membrane is an standard micro-fabrication process 37,38 .…”

Section: Microscale Direct Measurement Of Localized Photothermal Heatmentioning

confidence: 99%

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

Davaji

Richie

Lee

2019

Sci Rep

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Photothermal hyperthermia is proven to be an effective diagnostic tool for cancer therapy. The efficacy of this method directly relies on understanding the localization of the photothermal effect in the targeted region. Realizing the safe and effective concentration of nano-particles and the irradiation intensity and time requires spatiotemporal temperature monitoring during and after laser irradiation. Due to uniformities of the nanoparticle distribution and the complexities of the microenvironment, a direct temperature measurement in micro-scale is crucial for achieving precise thermal dose control. In this study, a 50 nm thin film nickel resistive temperature sensor was fabricated on a 300 nm SiN membrane to directly measure the local temperature variations of a hydrogel-GNR mixture under laser exposure with 2 mK temperature resolution. The chip-scale approach developed here is an effective tool to investigate localization of photothermal heating for hyperthermia applications for in-vitro and ex-vivo models. Considering the connection between thermal properties, porosity and the matrix stiffness in hydrogels, we present our results using the interplay between matrix stiffness of the hydrogel and its thermal properties: the stiffer the hydrogel, the higher the thermal conductivity resulting in lower photothermal heating. We measured 8.1, 7.4, and 5.6 °C temperature changes (from the room temperature, 20 °C) in hydrogel models with stiffness levels corresponding to adipose (4 kPa), muscle (13 kPa) and osteoid (30 kPa) tissues respectively by exposing them to 2 W/cm 2 laser (808 nm) intensity for 150 seconds.

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Section: Microscale Direct Measurement Of Localized Photothermal Heatmentioning

confidence: 99%

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

Davaji

Richie

Lee

2019

Sci Rep

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A patterned single layer graphene resistance temperature sensor

Davaji

Cho

Malakoutian

et al. 2017

Sci Rep

126

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Micro-fabricated single-layer graphenes (SLGs) on a silicon dioxide (SiO2)/Si substrate, a silicon nitride (SiN) membrane, and a suspended architecture are presented for their use as temperature sensors. These graphene temperature sensors act as resistance temperature detectors, showing a quadratic dependence of resistance on the temperature in a range between 283 K and 303 K. The observed resistance change of the graphene temperature sensors are explained by the temperature dependent electron mobility relationship (~T−4) and electron-phonon scattering. By analyzing the transient response of the SLG temperature sensors on different substrates, it is found that the graphene sensor on the SiN membrane shows the highest sensitivity due to low thermal mass, while the sensor on SiO2/Si reveals the lowest one. Also, the graphene on the SiN membrane reveals not only the fastest response, but also better mechanical stability compared to the suspended graphene sensor. Therefore, the presented results show that the temperature sensors based on SLG with an extremely low thermal mass can be used in various applications requiring high sensitivity and fast operation.

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On-chip detection of gel transition temperature using a novel micro-thermomechanical method

et al. 2017

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We present a new thermomechanical method and a platform to measure the phase transition temperature at microscale. A thin film metal sensor on a membrane simultaneously measures both temperature and mechanical strain of the sample during heating and cooling cycles. This thermomechanical principle of operation is described in detail. Physical hydrogel samples are prepared as a disc-shaped gels (200 μm thick and 1 mm diameter) and placed between an on-chip heater and sensor devices. The sol-gel transition temperature of gelatin solution at various concentrations, used as a model physical hydrogel, shows less than 3% deviation from in-depth rheological results. The developed thermomechanical methodology is promising for precise characterization of phase transition temperature of thermogels at microscale.

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In-vivo single cell protein interaction investigation using microfluidic platform

Cited by 3 publications

References 13 publications

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

Microscale direct measurement of localized photothermal heating in tissue-mimetic hydrogels

A patterned single layer graphene resistance temperature sensor

On-chip detection of gel transition temperature using a novel micro-thermomechanical method

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