In vivo replication kinetics and transcription patterns of the nucleopolyhedrovirus (NeabNPV) of the balsam fir sawfly, Neodiprion abietis

Abstract

In vivo replication kinetics and transcription patterns of the nucleopolyhedrovirus (NeabNPV) of the balsam fir sawfly, Neodiprion abietis

Recent Advances in Our Knowledge of Baculovirus Molecular Biology and Its Relevance for the Registration of Baculovirus-Based Products for Insect Pest Population Control

“…ODVs initiate midgut cell infection and once infected, the midgut cell nuclei become the site of viral gene expression and DNA replication in a manner that is consistent with that described for alphabaculoviruses (Duffy et al, 2007). In infected balsam fir sawfly larvae, Neodiprion abietis NPV (NeabNPV) viral DNA increased over 200% within the first 2 hours post infection (hpi) (Duffy et al, 2007). Progeny virions were occluded directly without the production of BVs (reviewed in Federici, 1997;Slack & Arif, 2007;Rohrmann, 2011).…”

“…(See section 3.7.) The NeabNPV replication cycle is rapid and efficient, with over 100 nucleocapsids being occluded in each OB (Duffy et al, 2007). Rather than using the BV phenotype to propagate the initial infection to other midgut cells, the released OBs have been suggested to serve as inoculum for other gut cells (Rohrmann, 2011).…”

Pathology of aGammabaculovirusin Its Natural Balsam Fir Sawfly (Neodiprion abietis) Host

“…Larvae were held, by hand, using insect forceps (Fine Science Tools, Foster City, CA, USA) and were presented the droplet at the end of a 1 cc, 27G1/2 syringe and needle (Becton Dickinson, Franklin Lakes, NJ) mounted on an automicroapplicator (Burkard Manufacturing, Rickmansworth, UK). Each droplet contained approximately 1 × 10 4 OBs [23]. Control larvae were fed single 1 µL 10% honey droplets without OBs.…”

“…• C) prior to being manually fed a 1 µL droplet of NeabNPV OBs suspended in a 10% aqueous solution of pasteurized liquid honey [23]. Larvae were held, by hand, using insect forceps (Fine Science Tools, Foster City, CA, USA) and were presented the droplet at the end of a 1 cc, 27G1/2 syringe and needle (Becton Dickinson, Franklin Lakes, NJ) mounted on an automicroapplicator (Burkard Manufacturing, Rickmansworth, UK).…”

“…Unprecedented outbreaks of balsam fir sawfly populations on the island of Newfoundland (Newfoundland and Labrador (NL), Canada) [17] have led to the development, registration, commercialization, and use Psyche of an N. abietis-specific Gammabaculovirus (NeabNPV) [18][19][20][21]. The genome of NeabNPV has been sequenced [22], and the in vivo replication and transcription kinetics studied [23]. There is, however, a dearth of information on the pathology of sawfly NPVs in the literature, although they are thought to only infect the midgut epithelium of their hosts [24,25].…”