In vivo remodeling of human cell-assembled extracellular matrix yarns

Magnan, Laure; Kawecki, Fabien; Labrunie, Gaëlle; Gluais, Maude; Izotte, Julien; Marais, Sébastien; Foulc, Marie-Pierre; Lafourcade, Mickaël; L’Heureux, Nicolas

doi:10.1016/j.biomaterials.2021.120815

Cited by 9 publications

(20 citation statements)

References 35 publications

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“…[53][54][55] Our bioengineering process relies on producing a truly "bio" material we term the Cell-Assembled extracellular Matrix (CAM). 27,[56][57][58][59] The quality of this ECM, endogenously secreted and assembled by the fibroblasts of the patients themselves in culture, is critical to the success of the engineered vessel. This study sought to ascertain the inter-donor variability of the CAM produced by fibroblasts derived from 21 patients for whom tissue-engin- eered vascular grafts were produced as part of a clinical trial.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 This CAM can be seen as a new type of biomaterial and is produced as a sheet that can be processed into different shapes such as tubes, [14][15][16][17][18][19][20]23 valve leaflets, 24,25 or yarns. 26,27 We have pioneered the use of the human CAM for autologous and allogeneic vascular applications. 14,17,18,20 Rolled CAM sheets were fused to produce tissue-engineered blood vessels under a good manufacturing practice (GMP) for hemodialysis access.…”

Section: Introductionmentioning

confidence: 99%

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Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts

et al. 2022

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts

et al. 2022

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“…The strength of this material also means that it can be easily transplanted in a host, including allowing the use of surgical sutures and staples. But more importantly, this material is not recognized as damaged ECM, like chemicallyprocessed ECM proteins, and will not be aggressively degraded [31]. For this reason, we hypothesized that the fine structures that were assembled in vitro, using CAM as a scaffold, could be preserved once implanted in the host.…”

Section: Discussionmentioning

confidence: 99%

“…The CAM is made of a dense network of fibrillar collagen, which is responsible for its mechanical strength, but also includes a wide array of other ECM molecules to support biological functions [20]. These CAM biomaterials have shown to be easily devitalized and processed without loss of their mechanical properties, displayed many tissue-like properties that could support normal cell-ECM interactions, and allow implantation without triggering degradative responses from the host's innate immune system [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Cell-assembled extracellular matrix (CAM): a human biopaper for the biofabrication of pre-vascularized tissues able to connect to the host circulation in vivo

Oliveira¹,

Médina²,

Labrunie³

et al. 2021

Biofabrication

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When considering regenerative approaches, the efficient creation of a functional vasculature, that can support the metabolic needs of bioengineered tissues, is essential for their survival after implantation. However, it is widely recognized that the post-implantation microenvironment of the engineered tissues is often hypoxic due to insufficient vascularization, resulting in ischemia injury and necrosis. This is one of the main limitations of current tissue engineering applications aiming at replacing significant tissue volumes. Here, we have explored the use of a new biomaterial, the cell-assembled extracellular matrix (CAM), as a biopaper to biofabricate a vascular system. CAM sheets are a unique, fully biological and fully human material that has already shown stable long-term implantation in humans. We demonstrated, for the first time, the use of this unprocessed human ECM as a microperforated biopaper. Using microvalve dispensing bioprinting, concentrated human endothelial cells (30 millions ml−1) were deposited in a controlled geometry in CAM sheets and cocultured with HSFs. Following multilayer assembly, thick ECM-based constructs fused and supported the survival and maturation of capillary-like structures for up to 26 d of culture. Following 3 weeks of subcutaneous implantation in a mice model, constructs showed limited degradative response and the pre-formed vasculature successfully connected with the host circulatory system to establish active perfusion.This mechanically resilient tissue equivalent has great potential for the creation of more complex implantable tissues, where rapid anastomosis is sine qua non for cell survival and efficient tissue integration.

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“…Due to their different appearance, the decellularization protocols of the two ECMs differ in the used solutions and time by default in current studies (Flynn, 2010;Guneta et al, 2018;Magnan et al, 2018Magnan et al, , 2021Song et al, 2018). In this study, dECM was decellularized using relatively harsh chemicals and enzymes (no detergents which are classified as critical were used) whereas cdECM was treated with a relatively gentle hypotonic solution and nucleases.…”

Section: General Aspectsmentioning

confidence: 99%

Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

Nellinger

Mrsic

Keller

et al. 2022

Biotech & Bioengineering

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Due to its availability and minimal invasive harvesting human adipose tissue‐derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell‐derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose‐derived stem cells, toward their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans compared with cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM‐based biomaterial for their specific application and the interpretation of obtained results.

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In vivo remodeling of human cell-assembled extracellular matrix yarns

Cited by 9 publications

References 35 publications

Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts

Inter-donor variability of extracellular matrix production in long-term cultures of human fibroblasts

Cell-assembled extracellular matrix (CAM): a human biopaper for the biofabrication of pre-vascularized tissues able to connect to the host circulation in vivo

Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

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