In Vivo Release of Previously Cleared Interferon by Cycloheximide

Abstract

The release of previously cleared interferon by cycloheximide was studied in the mouse. When cycloheximide was administered after either endogenous interferon stimulation or administration of exogenous interferon, the clearance of interferon from the blood stream was interrupted and a sharp rise in interferon titer occurred approximately 6 hr after cycloheximide administration followed by a rapid decline to low levels. This effect was observed with either interferon stimulated endogenously (by polyriboinosinic polyribocytidylic acid), or homologous (mouse) or heterologous (rabbit) interferon administered exogenously. Serum protein concentrations also exhibited this rise and fall phenomenon after cycloheximide administration although the magnitude of the change in protein concentrations was less pronounced than that observed with interferon. Hematocrits, although elevated in mice receiving cycloheximide, did not exhibit this rise and fall phenomenon. Hence, cycloheximide administration leads to the release into the circulation of previously cleared interferon as well as other proteins. Cycloheximide was purchased from Sigma 383 on August 2, 2020 by guest http://iai.asm.org/ Downloaded from 102 132 102 138 a Dose: 150 mg/kg in 0.5 ml of distilled water. 386 INFECT. IMMUNITY on August 2, 2020 by guest http://iai.asm.org/ Downloaded from

Effect of lead on interferon production and antiviral effect of interferon on Marek's disease in chickens

“…The spleen, bone-marrow, lung and to a lesser extent It is possible that low levels of interferon produced by various stimuli (e.g. gut bacterial endotoxin or viral infections) in animals keep the clearance mechanism at least partially saturated with interferon which is released by the cell-destructive effects of cycloheximide (30).…”

Effect of lead on interferon production and antiviral effect of interferon on Marek's disease in chickens

“…One involves the novo synthesis of interferon and the other the release of prefcnaed interferon (110) It is possible that low levels of interferon produced by various stimuli (e.g. gut bacterial endotoxin or viral infections) in animals keep the clearance mechanism at least partially saturated with interferon which is released by the cell-destructive effects of cycloheximide (30).…”