In vivo release of newly synthesized catecholamines from the hypothalamus by amphetamine

Philippu, A.; Głowiński, J.; Besson, Marie‐Jo

doi:10.1007/bf00647398

Cited by 20 publications

(3 citation statements)

References 11 publications

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“…Since that time, various attempts have been made to use and improve this perfusion device in order to study the release of neurotransmitters in the CNS (BARTHOLINI & STADLER, 1975a,b;DE FEUDIS et al, 1970;MYERS, 1970;MYERS & BELESLIN, 1970;PHILIPPU et a/., 1974;SzERB, 1967). Valuable data were obtained, but in most cases the levels of transmitter in superfusates were low and few histological pictures were published (BARTHOLINI & STADLER, 1964;MYERS & BELESLIN, 1970;PHILIPPU et at., 1974;RIDDELL & SZERB, 1971;ROTH et al, 1969). In order to use this local superfusion method, we have tested various types of push-pull cannula.…”

Section: Discussionmentioning

confidence: 99%

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

Nieoullon

Chéramy

Głowiński

1977

Journal of Neurochemistry

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144

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Abstract— The release of [3H]dopamine ([3H]DA) continuously synthesized from l‐[3,5–3H]tyrosine from the caudate nucleus of the cat was estimated in halothane anaesthetized or‘encéphale isolé’animals. For this purpose, an improved superfusion cannula, avoiding tissue damage, was used. The best localization for the tip of the superfusion cannula was found first by determining the topographical distribution of endogenous DA within the caudate nucleus. A rostro‐caudal heterogenous distribution of the transmitter was detected. In perfusion experiments, l‐[3,5–3H]tyrosine was introduced continuously at a rate of 33μl/min. [3H]DA was the only catecholamine found in serial 15 min fractions as revealed by cochromatography. The spontaneous release of [3H]DA was greater in anaesthetized than in ‘encéphale isolé’ cats; it represented 150 and 100 times the blank value, respectively. Depolarization by K+ (30 mm) applied locally in the striatum or by electrical or mechanical stimulation of the substantia nigra caused a transitory increase in [3H]DA release. Conversely, a decrease in nerve activity induced by tetrodotoxin (5 × 10−‐7 m) or by electrocoagulation of the substantia nigra was associated with a decline in the amounts of [3H]DA in superfusates. A temporary reduction in [3H]DA release could also be obtained by a short‐lasting cooling block of the substantia nigra. As expected, d‐amphetamine (10−‐5 m) and benzotropine(10−‐7 m) added to the superfusing medium increased [3H]DA release. These pharmacological results, as well as the changes in [3H]DA release observed after various manipulations of the activity of dopaminergic neurones, confirms the validity and the high sensitivity of this approach.

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Section: Discussionmentioning

confidence: 99%

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

Nieoullon

Chéramy

Głowiński

1977

Journal of Neurochemistry

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144

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“…This com partment correlates well with a functional more rapidly turning over NE store [Kopin et al. 1968;Philippu et al. 1974], The addi tional loading procedures were performed by incubating tissues in NE-'H prior to either a brief 25-min wash (NE-'H) or prolonged 180-min wash (NE-'H) period in amine-free Krebs-Ringer bicarbonate.…”

Section: Discussionmentioning

confidence: 99%

Sympathomimetic Amine-Induced Release of Norepinephrine-<sup>3</sup>H from Different Intraneuronal Storage Compartments

Takimoto¹,

Amiri²,

Cho³

1981

Pharmacology

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The effects of different loading procedures on norepinephrine-³H (NE-³H) efflux induced by a structurally analogous group of indirectly acting sympathomimetic amines was examined in rabbit vas deferens. (+)-Amphetamine was ineffective in releasing ³H from tissues with intact intraneuronal storage granules but was an effective depletor of (a) non-granular intraneuronal compartment(s) following in vivo treatment with reserpine. (±)-p-Hy-droxynorephedrine was a less effective depletor of non-granular NE-³H. These experiments provide further evidence in support of multiple intraneuronal storage compartments which can be pharmacologically differentiated by utilizing indirectly acting sympathomimetic amine agents.

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“…D-Amphetamine (10-6 M) caused release of 3H-NA taken up either by chopped tissue, homogenates, or synaptosomes from rat hypothalamus (PHILIPPU et al, 1974) and cerebral cortex (RUTLEDGE et al, 1972a(RUTLEDGE et al, , b, 1973AZZARO and RUTLEDGE, 1973;AZZARO et al, 1974;. D-Amphetamine (10-6 M) caused release of 3H-NA taken up either by chopped tissue, homogenates, or synaptosomes from rat hypothalamus (PHILIPPU et al, 1974) and cerebral cortex (RUTLEDGE et al, 1972a(RUTLEDGE et al, , b, 1973AZZARO and RUTLEDGE, 1973;AZZARO et al, 1974;.…”

Section: Adrenergic Transmissionmentioning

confidence: 99%

General Pharmacology of Amphetamine-Like Drugs

Änggård¹,

Lewander²,

Gunne³

1973

Drug Addiction II

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Amphetamine and related central stimulant drugs are derivatives of ß-phenylethylamine (Table 1) and are thus relatively simple organic bases. The general ßphenylethylamine skeleton is one which amphetamine shares with the neurotransmitters noradrenaline, adrenaline, and dopamine. Phenylethylamine itselfhas central stimulant properties, but has an extremely short half-life in the body due to rapid metabolism by monoamine oxidase (MAO). Amphetamine has, due to steric hindrance by the ex-methyl group, much less affinity for MAO and therefore has a longer half-Iife.Most amphetamines have cardiovascular, psychomotor stimulant, hyperthermic, and anorexigenic actions. All of the structural features of amphetamine are important far its spectrum of pharmacologic activity. Any alteration may enhance, diminish, or attenuate one or several components in the actions of the parent drug. (1) Substitution of the phenyl ring, (2) alteration of the length of the side chain, (3) substitution on the primary nitrogen group, (4) substitution of the ex-and ß-carbon atoms, and (5) their absolute configuration have been studied and will be briefly discussed here. For a more thorough review on the subject the reader is referred to the papers by BIEL (1970), vANRossuM and SIMONS (1969) and VREE (1973. The effect of ring and side-chain substitution on distribution and elimination of the amphetamines has been discussed by BECKETT and BROOKES (1970). recently reviewed the biochemical pharmacology of the amphetamines. The metabolism and disposition of methylphenidate were reported by F ARAJ et al.

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In vivo release of newly synthesized catecholamines from the hypothalamus by amphetamine

Cited by 20 publications

References 11 publications

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

Sympathomimetic Amine-Induced Release of Norepinephrine-<sup>3</sup>H from Different Intraneuronal Storage Compartments

General Pharmacology of Amphetamine-Like Drugs

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In vivo release of newly synthesized catecholamines from the hypothalamus by amphetamine

Cited by 20 publications

References 11 publications

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [3H]DOPAMINE SYNTHESIZED FROM [3H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [3H]DOPAMINE SYNTHESIZED FROM [3H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

Sympathomimetic Amine-Induced Release of Norepinephrine-<sup>3</sup>H from Different Intraneuronal Storage Compartments

General Pharmacology of Amphetamine-Like Drugs

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AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS

AN ADAPTATION OF THE PUSH‐PULL CANNULA METHOD TO STUDY THE IN VIVO RELEASE OF [³H]DOPAMINE SYNTHESIZED FROM [³H]TYROSINE IN THE CAT CAUDATE NUCLEUS: EFFECTS OF VARIOUS PHYSICAL AND PHARMACOLOGICAL TREATMENTS