In vivo regulation of hepatic lipase activity and mRNA levels by diets which modify cholesterol influx to the liver

Benhizia, Ferdaous; Lagrange, Dominique; Malewiak, Marie-Irène; Griglio, S.

doi:10.1016/0005-2760(94)90267-4

Cited by 19 publications

(9 citation statements)

References 49 publications

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“…HL hydrolyzes TG and PL of small lipoproteins, and consequently influence metabolism of high‐density lipoprotein (HDL), low‐density lipoprotein (LDL), and IDL (Santamarina‐Fojo et al., 1998). The activities of these lipases are regulated in part at a transcriptional level in response to the physiological state of animals (Benhizia et al., 1994; Ruge et al., 2004). Serum contains a considerable LPL mass, but the activity of TG hydrolysis is very low.…”

Section: Introductionmentioning

confidence: 99%

The role of lipase in blood lipoprotein metabolism and accumulation of lipids in oocytes of the Siberian sturgeon Acipenser baerii during maturation

Zhang

Zhuang

et al. 2011

Journal of Applied Ichthyology

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The purpose of this study was to investigate the role of lipoprotein lipase (LPL) and hepatic lipase (HL) played in accumulating lipid into oocytes and serum lipid metabolism in Siberian sturgeon Acipenser baerii. We determined ovarian LPL activity, serum LPL and HL activity, and several serum lipid amounts in different ovary development stages. Our results shown that LPL activity in ovaries during vitellogenesis, serum LPL and HL activity were increased during vitellogenesis with a significant high level in stage V, and decreased fast after ovulation. Serum very low density lipoprotein (VLDL), triglycerides (TG), and phospholipids (PL) concentrations were increased since stage II to IV, and then decreased till stage VI. Serum cholesterol (TC) and lowdensity lipoprotein (LDL) amounts were gradually increasing during ovaries development. Moreover, we found that serum two lipases activity positively correlated with serum VLDL, TG and, TC and negatively correlated with high-density lipoprotein (HDL). The variation tendency of serum LPL, HL activity and lipids concentration, and the close correlationship between the two lipases and lipids during ovarian development suggest that LPL and HL participate in regulating blood lipid metabolism.

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Section: Introductionmentioning

confidence: 99%

The role of lipase in blood lipoprotein metabolism and accumulation of lipids in oocytes of the Siberian sturgeon Acipenser baerii during maturation

Zhang

Zhuang

et al. 2011

Journal of Applied Ichthyology

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“…In HepG2 cells, atorvastatin as well as forced expression of nSREBP2 reduced HL secretion and HL gene transcription [23]. However, feeding rats a cholesterol-enriched diet, which suppresses SREBP activity, was also reported to reduce HL expression [28]. Our previous studies using HepG2 cells suggested that SREBP2 interferes with the sensitivity of the HL gene to upregulation by Upstream Stimulatory Factors (USFs) [23].…”

Section: Introductionmentioning

confidence: 99%

Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1

et al. 2009

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Polyunsaturated fatty acids affect gene expression mainly through peroxisome proliferator-activated receptors (PPARs) and sterol regulatory element binding proteins (SREBPs), but how monounsaturated fatty acids affect gene expression is poorly understood. In HepG2 cells, oleate supplementation has been shown to increase secretion of hepatic lipase (HL). We hypothesized that oleate affects HL gene expression at the transcriptional level. To test this, we studied the effect of oleate on HL promoter activity using HepG2 cells and the proximal HL promoter region (700 bp). Oleate increased HL expression and promoter activity 1.3–2.1 fold and reduced SREBP activity by 50%. Downregulation of SREBP activity by incubation with cholesterol+25-hydroxycholesterol had no effect on HL promoter activity. Overexpression of SREBP2, but not SREBP1, reduced HL promoter activity, which was effected mainly through the USF1 binding site at -307/-312. Oleate increased the nuclear abundance of USF1 protein 2.7 ± 0.6 fold, while USF1 levels were reduced by SREBP2 overexpression. We conclude that oleate increases HL gene expression via USF1. USF1 may be an additional fatty acid sensor in liver cells.

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“…The results of simvastatin on serum TG levels could be explained through elevation of lipoprotein lipase activity by increasing lipase mRNA expression [41] and through suppression of diacyl glycerol acyl transferase, which catalyzes the final step in TG biosynthesis in the rat liver microsomes [26,27,42]. Here, administration of mushroom extract, chrysin, curcumin and omega-3 as well as simvastatin to hypercholesterolemic rats resulted in reduction of serum levels of total cholesterol and TG, and elevation of HDLcholesterol than those in hypercholesterolemic rats.…”

Section: Discussionmentioning

confidence: 99%

Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats

Ismail¹,

Soliman²,

Nassan³

et al. 2015

JFNR

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Hypercholesterolemia and hypertriglyceridemia are major risk factors that accelerate the incidence of atherosclerosis and coronary artery diseases. Therefore, the present study was conducted to evaluate the hypolipidemic effect of widely known traditional medicinal herbs and omega-3 FA in experimental hypercholesterolemia induced by Triton WR-1339. Experimental hypercholesterolemic rats were administered mushroom, chrysin, curcumin and omega-3 for 2 weeks. Hypercholesterolemic rats showed an increase in serum levels of lipid profiles and hepatic enzymes. Hypercholesterolemic rats showed an increase in malondialdehyde (MDA) levels and a decrease in both serum levels and mRNA expression of catalase, superoxide dismutase (SOD) and glutathione reducatse. Moreover, hypercholesterolemic rats showed hepatic down regulation in the expression of genes related to fatty acids oxidation such as acyl CoA oxidase (ACO) and synthetase (ACS), together with carnityl palmityl transferase-1 (CPT-1) and peroxisome proliferator activator receptor-α (PPAR-α). Administration of mushroom, chrysin, curcumin and omega-3 to hypercholesterolemic rats for 2 weeks up-regulated significantly the down regulated genes. In contrast, expression of genes related to fatty acids biosynthesis and cholesterol metabolism were increased in hypercholesterolemic rats compared to control group. Herbal medications and omega-3 administration down regulated genes of fatty acids biosynthesis and cholesterol metabolism to normal expression. At cellular levels, hyperlipidemia induced fatty droplets accumulation, necrosis and presence of apoptotic hepatocytes together with leukocytic infiltration in necrotic area that are ameliorated and normalized after administration of herbs and omega-3. In conclusion, the current findings indicated that flavonoids (mushroom, chrysin, curcumin) and omega-3 possess antihypercholesterolemic effects at biochemical, molecular and histopathological levels and are useful in treatment of hypercholesterolemia with lower side effects compared with synthetic hypolipidemic drugs.

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In vivo regulation of hepatic lipase activity and mRNA levels by diets which modify cholesterol influx to the liver

Cited by 19 publications

References 49 publications

The role of lipase in blood lipoprotein metabolism and accumulation of lipids in oocytes of the Siberian sturgeon Acipenser baerii during maturation

The role of lipase in blood lipoprotein metabolism and accumulation of lipids in oocytes of the Siberian sturgeon Acipenser baerii during maturation

Activation of Hepatic Lipase Expression by Oleic Acid: Possible Involvement of USF1

Antihypercholesterolemic Effects of Mushroom, Chrysin, Curcumin and Omega-3 in Experimental Hypercholesterolemic Rats

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