In vivo Recording Quality of Mechanically Decoupled Floating Versus Skull-Fixed Silicon-Based Neural Probes

Chauvière, Laëtitia; Pothof, Frederick; Gansel, Kai S.; Klon-Lipok, Johanna; Aarts, Arno; Holzhammer, Tobias; Oliver, Paul; Singer, Wolf; Ruther, Patrick

doi:10.3389/fnins.2019.00464

Cited by 14 publications

(11 citation statements)

References 54 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…due to blood pulsation or an acceleration of the skull, cannot be prohibited. This will cause a relative movement between the chamber-fixed neural probe and the surrounding tissue, affecting the signal quality and ultimately influencing the long-term recording capability (Chauvière et al, 2019;Pothof et al, 2017). In order to minimize this brain movement, a cylindrical plug, as shown in Figures 1(A) and 3(A), is used in our approach.…”

Section: Microdrivementioning

confidence: 99%

See 1 more Smart Citation

High-density electrophysiological recordings in macaque using a chronically implanted 128-channel passive silicon probe

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective -The analysis of interactions among local populations of neurons in the cerebral cortex (e.g. within cortical microcolumns) requires high resolution and high channel count recordings from chronically implanted laminar microelectrode arrays. The request for high-density recordings of a large number of recording sites can presently only be accomplished by probes realized using complementary metal-oxide-semiconductor (CMOS) technology. In preparation for their use in nonhuman primates, we aimed for neural probe validation in a head-fixed approach analyzing the longterm recording capability. Approach -We examined chronically implanted silicon-based laminar probes, realized using a CMOS technology in combination with micromachining, to record from the primary visual cortex (V1) of a monkey. We used a passive CMOS probe that had 128 electrodes arranged at a pitch of 22.5 µm in four columns and 32 rows on a slender shank. In order to validate the performance of a dedicated microdrive, the overall dimensions of probe and interface boards were chosen to be compatible with the final active CMOS probe with integrated circuitry. Main results -Using the passive probe, we recorded simultaneously local field potentials (LFP) and spiking multiunit activity (MUA) in V1 of an awake behaving macaque monkey. We found that an insertion through the dura and subsequent readjustments of the chronically implanted neural probe was possible and allowed us to record stable LFPs for more than five months. The quality of MUA degraded within the first month but remained sufficiently high to permit mapping of receptive fields during the full recording period. Significance -We conclude that the passive silicon probe enables semi-chronic recordings of high quality of LFP and MUA for a time span exceeding five months. The new microdrive compatible with a commercial recording chamber successfully demonstrated the readjustment of the probe position while the implemented plug structure effectively reduced brain tissue movement relative to the probe.

show abstract

Section: Microdrivementioning

confidence: 99%

“…After a preceding study (Chauvière et al, 2019) was finished, the microdrive and probe of that study were removed, and the sleeve with silastic membrane was exchanged. A plug (Gray et al, 2007) was inserted into the chamber, which was sealed with a flat titanium cap.…”

Section: Surgerymentioning

confidence: 99%

High-density electrophysiological recordings in macaque using a chronically implanted 128-channel passive silicon probe

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Probes with thin, flexible connections to the outside world are commonly termed 'floating' probes which exploit microwires or polyimide cables [1][2][3]. Despite the opportunity to decrease micromotion by foregoing tethering to the skull, the short-lived improvement in recording stability has been dismissed as insufficient justification for the design of floating probes, especially when considering the stringent requirements (shielding and mechanical robustness) for highfrequency communication wires [4]. This work prioritizes a flexible polymer substrate as the first step towards a truly integrated probe for a closed-loop system.…”

Section: Introductionmentioning

confidence: 99%

Neural microprobe modelling and microfabrication for improved implantation and mechanical failure mitigation

McGlynn

Walton

Das

et al. 2022

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Careful design and material selection are the most beneficial strategies to ensure successful implantation and mitigate the failure of a neural probe in the long term. In order to realize a fully flexible implantable system, the probe should be easily manipulated by neuroscientists, with the potential to bend up to 90°. This paper investigates the impact of material choice, probe geometry, and crucially, implantation angle on implantation success through finite-element method simulations in COMSOL Multiphysics followed by cleanroom microfabrication. The designs introduced in this paper were fabricated using two polyimides: (i) PI-2545 as a release layer and (ii) photodefinable HD-4110 as the probe substrate. Four different designs were microfabricated, and the implantation tests were compared between an agarose brain phantom and lamb brain samples. The probes were scanned in a 7 T PharmaScan MRI coil to investigate potential artefacts. From the simulation, a triangular base and 50 µm polymer thickness were identified as the optimum design, which produced a probe 57.7 µm thick when fabricated. The probes exhibit excellent flexibility, exemplified in three-point bending tests performed with a DAGE 4000Plus. Successful implantation is possible for a range of angles between 30° and 90°. This article is part of the theme issue ‘Advanced neurotechnologies: translating innovation for health and well-being’.

show abstract

“…The general fabrication steps involve the electrode alignment which counts on manual work with grids and hands-on small wire soldering [ 26 , 27 , 28 ], and both procedures are time-consuming. Such a labor-intensive fabrication process has driven many neuroscientists toward commercially available microwire MEAs [ 29 , 30 ]. These arrays, while ready off-the-shelf, require recording experiments to be designed around the available configurations [ 22 , 31 ] and would result in higher costs and a longer lead time for customized MEA geometry needs.…”

Section: Introductionmentioning

confidence: 99%

3D Printed Skull Cap and Benchtop Fabricated Microwire-Based Microelectrode Array for Custom Rat Brain Recordings

Hartner

Ung

et al. 2022

Bioengineering

View full text Add to dashboard Cite

Microwire microelectrode arrays (MEAs) have been a popular low-cost tool for chronic electrophysiological recordings and are an inexpensive means to record the electrical dynamics crucial to brain function. However, both the fabrication and implantation procedures for multi-MEAs on a single rodent are time-consuming and the accuracy and quality are highly manual skill-dependent. To address the fabrication and implantation challenges for microwire MEAs, (1) a computer-aided designed and 3D printed skull cap for the pre-determined implantation locations of each MEA and (2) a benchtop fabrication approach for low-cost custom microwire MEAs were developed. A proof-of-concept design of a 32-channel 4-MEA (8-wire each) recording system was prototyped and tested through Sprague Dawley rat recordings. The skull cap design, based on the CT-scan of a single rat conforms well with multiple Sprague Dawley rats of various sizes, ages, and weight with a minimal bregma alignment error (A/P axis standard error of the mean = 0.25 mm, M/L axis standard error of the mean = 0.07 mm, n = 6). The prototyped 32-channel system was able to record the spiking activities over five months. The developed benchtop fabrication method and the 3D printed skull cap implantation platform would enable neuroscience groups to conduct in-house design, fabrication, and implantation of customizable microwire MEAs at a lower cost than the current commercial options and experience a shorter lead time for the design modifications and iterations.

show abstract

In vivo Recording Quality of Mechanically Decoupled Floating Versus Skull-Fixed Silicon-Based Neural Probes

Cited by 14 publications

References 54 publications

High-density electrophysiological recordings in macaque using a chronically implanted 128-channel passive silicon probe

High-density electrophysiological recordings in macaque using a chronically implanted 128-channel passive silicon probe

Neural microprobe modelling and microfabrication for improved implantation and mechanical failure mitigation

3D Printed Skull Cap and Benchtop Fabricated Microwire-Based Microelectrode Array for Custom Rat Brain Recordings

Contact Info

Product

Resources

About