In vivo reconstitution of autophagy in <i>Saccharomyces cerevisiae </i>

Cao, Yang; Cheong, Heesun; Song, Hui; Klionsky, Daniel J.

doi:10.1083/jcb.200801035

Cited by 64 publications

(74 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To address this point, we took advantage of a strain developed previously, the multiple-knockout (MKO) strain, which lacks 24 different ATG genes that are required for autophagosome formation. 43,44 The re-addition to this strain of specific genes in various combinations allows the "in vivo reconstitution" of autophagy, and the definition of the minimal set of proteins necessary for a specific step of this process. 45 Recently we showed that the MKO strain expressing Atg9, Atg11, Atg23 and Atg27 was sufficient to promote the anterograde movement of Atg9.…”

Section: Phosphorylation Of Atg9 S122 Is Important For Selective Automentioning

confidence: 99%

Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation

Feng¹,

Backues²,

Baba³

et al. 2016

Autophagy

Self Cite

View full text Add to dashboard Cite

Macroautophagy is primarily a degradative process that cells use to break down their own components to recycle macromolecules and provide energy under stress conditions, and defects in macroautophagy lead to a wide range of diseases. Atg9, conserved from yeast to mammals, is the only identified transmembrane protein in the yeast core macroautophagy machinery required for formation of the sequestering compartment termed the autophagosome. This protein undergoes dynamic movement between the phagophore assembly site (PAS), where the autophagosome precursor is nucleated, and peripheral sites that may provide donor membrane for expansion of the phagophore. Atg9 is a phosphoprotein that is regulated by the Atg1 kinase. We used stable isotope labeling by amino acids in cell culture (SILAC) to identify phosphorylation sites on this protein and identified an Atg1-independent phosphorylation site at serine 122. A nonphosphorylatable Atg9 mutant showed decreased autophagy activity, whereas the phosphomimetic mutant enhanced activity. Electron microscopy analysis suggests that the different levels of autophagy activity reflect differences in autophagosome formation, correlating with the delivery of Atg9 to the PAS. Finally, this phosphorylation regulates Atg9 interaction with Atg23 and Atg27.

show abstract

Section: Phosphorylation Of Atg9 S122 Is Important For Selective Automentioning

confidence: 99%

Phosphorylation of Atg9 regulates movement to the phagophore assembly site and the rate of autophagosome formation

Feng¹,

Backues²,

Baba³

et al. 2016

Autophagy

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, Tan et al proposed a general cellular mechanism for cargo recognition, which suggested that the K63 ubiquitin chain assembly is responsible for targeting intracellular inclusions for autophagy (31). Further, Cao et al recently determined the minimum requirements for cargo packaging using knock-out http://bmbreports.org BMB reports strains of S. cerevisiae (32).…”

Section: Cargo Recognitionmentioning

confidence: 99%

Autophagy-Is it a preferred route for lifespan extension?

Dwivedi

Ahnn

2009

BMB Reports

View full text Add to dashboard Cite

Autophagy, which is a process of self eating, has gained interest in the past decade due to its both beneficial and controversial roles in various biological phenomena. The discovery of autophagy genes (ATG) in yeast has led to focused research designed to elucidate the mechanism and regulation of this process. The role of autophagy in a variety of biological phenomena, including human disease, is still the subject of debate. However, recent findings suggest that autophagy is a highly regulated process with both beneficial and negative effects. Indeed, studies conducted using various model organisms have demonstrated that increased autophagy leads to an extended lifespan. Despite these findings, it is still unknown if all pathways leading to extended lifespan converge at the process of autophagy or not. Here, an overview of modern developments related to the process of autophagy, its regulation and the molecular machinery involved is presented. In addition, this review focuses on one of the beneficial aspects of autophagy, its role in lifespan regulation. [BMB reports 2009; 42(2): 65-71]

show abstract

“…Although none of these genes appear to be essential for growth under nutritionally replete conditions, most have proven to be indispensible during starvation. 1 During starvation, a form of bulk autophagy appears to harvest material indiscriminately. During other conditions, some of the same proteins, acting in conjunction with proteins recruited from other pathways, carry eukaryotes use a common set of genes to perform two mechanistically similar autophagic processes.…”

Section: Introductionmentioning

confidence: 99%