In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS)

Song, Yipeng; Talaty, Nari; Datsenko, Kirill A.; Wanner, Barry L.; Cooks, R. Graham

doi:10.1039/b900069k

Cited by 70 publications

(64 citation statements)

References 18 publications

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“…Biological applications of DESI-MS are rapidly growing, especially for cancer research [39,42,43], evaluation of lipid accumulation pathologies [44], the study of inflammatory responses [45], and botanical and microorganism analysis [46,47]. In parallel, multivariate analyses (such as PCA) plus customized software is being developed for the exploration and automated modeling of lipid data to aid in biological interpretation of DESI-MS data [39,48].…”

Section: Discussionmentioning

confidence: 99%

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

et al. 2012

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Knowledge of the lipids present in individual preimplantation embryos is of interest in fundamental studies of embryology, in attempts to understand cellular pluripotency and in optimization of in vitro culture conditions necessary for the application and development of biotechnologies such as in vitro fertilization and transgenesis. In this work, the profiles of fatty acids and phospholipids (PL) in individual mouse preimplantation embryos and oocytes were acquired using an analytical strategy based on desorption electrospray ionization mass spectrometry (DESI-MS). The methodology avoids sample preparation and provides information on the lipids present in these microscopic structures. Differences in the lipid profiles observed for unfertilized oocytes, two-and four-cell embryos, and blastocysts were characterized. For a representative set of embryos (N= 114) using multivariate analysis (specifically principal component analysis) unfertilized oocytes showed a narrower range of PL species than did blastocysts. Two-and four-cell embryos showed a wide range of PLs compared with unfertilized oocytes and high abundances of fatty acids, indicating pronounced synthetic activity. The data suggest that the lipid changes observed in mouse preimplantation development reflect acquisition of a degree of cellular membrane functional and structural specialization by the blastocyst stage. It is also noteworthy that embryos cultured in vitro from the two-cell through the blastocyst stage have a more homogeneous lipid profile as compared with their in vivo-derived counterparts, which is ascribed to the restricted diversity of nutrients present in synthetic culture media. The DESI-MS data are interpreted from lipid biochemistry and previous reports on gene expression of diverse lipids known to be vital to early embryonic development.

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Section: Discussionmentioning

confidence: 99%

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

et al. 2012

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“…46,47,68,71 Surfactin has antimicrobial, antifungal, and antiviral effects, and its secretion is crucial for the survival of B. subtilis in harsh environmental conditions. 72−76 Recently, the production of surfactin has been utilized as a distinguishing feature that differentiates various strains of B. subtilis.…”

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Rapid Discrimination of Bacteria by Paper Spray Mass Spectrometry

et al. 2014

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Paper spray mass spectrometry ambient ionization is utilized for rapid discrimination of bacteria without sample preparation. Bacterial colonies were smeared onto filter paper precut to a sharp point, then wetted with solvent and held at a high potential. Charged droplets released by field emission were sucked into the mass spectrometer inlet and mass spectra were recorded. Sixteen different species representing eight different genera from Gram-positive and Gram-negative bacteria were investigated. Phospholipids were the predominant species observed in the mass spectra in both the negative and positive ion modes. Multivariate data analysis based on principal component analysis, followed by linear discriminant analysis, allowed bacterial discrimination. The lipid information in the negative ion mass spectra proved useful for species level differentiation of the investigated Gram-positive bacteria. Gram-negative bacteria were differentiated at the species level by using a numerical data fusion strategy of positive and negative ion mass spectra.

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“…2,3 Imaging mass spectrometry (IMS) has emerged as a powerful tool for surface screening at the molecular level and different approaches are used. [2][3][4][5] "Invacuum" and ambient ionization have been constantly used for the imaging of microbial bioactive molecules. [6][7][8] Some "in-vacuum" techniques allow for the direct analysis of agar culture, such as those using matrix-assisted laser desorption ionization (MALDI) and secondary ion mass spectrometry (SIMS), however they can be time-consuming for data acquisition and/or sample preparation.…”

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Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture

et al. 2015

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Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.

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In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS)

Cited by 70 publications

References 18 publications

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

Developmental phases of individual mouse preimplantation embryos characterized by lipid signatures using desorption electrospray ionization mass spectrometry

Rapid Discrimination of Bacteria by Paper Spray Mass Spectrometry

Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture

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