In vivo real-time vessel imaging and ex vivo 3D reconstruction of atherosclerotic plaque in apolipoprotein E-knockout mice using synchrotron radiation microscopy

Three-Dimensional Imaging of Hepatic Sinusoids in Mice Using Synchrotron Radiation Micro-Computed Tomography

“…Reconstructed tomographic slice images consist of 1600 × 1200 pixels in the horizontal and vertical directions and the horizontal field of view is 580 µm. Using synchrotron radiation micro-CT, images with voxel size of approximately 1 µm are achievable [23,24]. …”

Phase contrast x-ray microscopy study of rabbit primo vessels

“…Phase contrast x-ray microscopy is used widely to examine the microstructural properties of biological systems, as well as condensed matters. [13][14][15] Unlike other techniques, phase contrast x-ray microscopy can describe the inside and outside of specimens in a wide range without destroying the specimen. Sample preparation and measurements take a relatively short period of time.…”

Introduction

“…These findings open up the possibilities for investigation of the efficacy of gene and pharmacological therapies for airway diseases, such as cystic fibrosis. Several studies have now also shown that PCXI techniques can be used to investigate atherosclerotic plaque composition without contrast agents 26,27 and have the sensitivity to detect lipid-rich areas based on tissue mass density. 27 However, non-invasive detection of plaques has yet to be demonstrated in living organisms without a contrast agent.…”