In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

Sato, Manabu; Eto, Kai; Masuta, Junpei; Inoue, Kenji; Kurotani, Reiko; Abé, Hiroyuki; Nishidate, Izumi

doi:10.3390/app9020216

Cited by 12 publications

(4 citation statements)

References 47 publications

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“…However, some technical disadvantages of the method prevent, for the moment, its brother use in clinical setups. We have already reported OCT as a promising tool for brain imaging (Osiac, Balseanu, Mogoanta, et al, 2014), but extensive and detailed investigations for clearer and unambiguous connection with the morphology and physiology of the observed processes, are still to be done (Osiac, Balseanu, Catalin, et al, 2014; Sato et al, 2019). As such we wanted to test its potential in a different neurological disorder, one that can have long‐lasting cellular changes in the brain (Donat, Scott, Gentleman, & Sastre, 2017; McColl et al, 2018; Witcher et al, 2018; Zhao et al, 2018) and a high cost of monitoring (van Dijck et al, 2019), in the hope that, with technological improvements, OCT could become a cheaper and faster alternative to MRI.…”

Section: Discussionmentioning

confidence: 99%

Optical coherence tomography microscopy in experimental traumatic brain injury

Osiac

Mitran

Manea

et al. 2020

Microscopy Res & Technique

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Worldwide elderly traumatic brain injury (TBI) patients tend to become an increasing burden to the society. Thus, a faster and less expensive way of evaluating TBI victims is needed. In the present study we investigated if optical coherence tomography (OCT) could be used as such a method. By using an animal model, we established if OCT can detect cortical changes in the acute phase of a penetrating TBI, in young (5–7 months) and old (20–22 months) rats. Due to the long‐term evolution of TBI's, we wanted to investigate to what extent OCT could detect changes within the cortex in the chronic phase. Adult (7–12 months) male rats were used. Surprisingly, OCT imaging of the normal hemisphere was able to discriminate age‐related differences in the mean gray values (MGV) of recorded pixels (p = .032). Furthermore, in the acute phase of TBI, OCT images recorded at 24 hr after the injury showed differences between the apparent damaged area of young and aged animals. Changes of MGV and skewness were only recorded 48 hr after injury. Monitoring the chronical evolution of the TBI with OCT revealed changes over time exceeding the normal range recorded for MGV, skewness and kurtosis, 14 and 21 days after TBI. Although in the present study we still used an extremely invasive approach, as technology improves, less invasive and non‐harmful ways of recording OCT may allow for an objective way to detect changes within the brain structure after brain injuries.

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Section: Discussionmentioning

confidence: 99%

Optical coherence tomography microscopy in experimental traumatic brain injury

Osiac

Mitran

Manea

et al. 2020

Microscopy Res & Technique

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“…To date, most FOISs have been cohesive multicore fiber bundles (CFBs) [9][10][11][12][13] consisting of thousands of fibers, each transmitting a single pixel. In recent years, imaging techniques based on a single multimode fiber (MMF) have attracted extensive research interest [14][15][16][17][18][19][20][21][22][23]. Compared with CFBs, an MMF supports the simultaneous propagation of over a hundred optical modes through a hair-thin imaging probe, reducing the invasiveness in imaging deep tissues and organs, which gives MMF-based imaging systems great potential in the field of medical endoscopy.…”

Section: Introductionmentioning

confidence: 99%

Enhanced ultrafine multimode fiber imaging based on mode modulation through singular value decomposition

Zhan,

Zhenming,

Cheng

et al. 2024

Photon. Res.

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The utilization of multimode fibers (MMFs) displays significant potential for advancing the miniaturization of optical endoscopes. However, the imaging quality is constrained by the physical conditions of MMF, which is particularly serious in small-core MMFs because of the limited mode quantity. To break this limitation and enhance the imaging ability of MMF to the maximum, we propose a mode modulation method based on the singular value decomposition (SVD) of MMF's transmission matrix (TM). Before injection into the MMF, a light beam is modulated by the singular vectors obtained by SVD. Because the singular vectors reflect the transfer characteristics of MMF and selectively excite the modes of different orders, the optimal distribution of the excited modes in MMF can be achieved, thereby improving the imaging quality of the MMF imaging system to the greatest extent. We conducted experiments on the MMF system with 40 µm and 105 µm cores to verify this method. Deep learning is utilized for image reconstruction. The experimental results demonstrate that the properties of the output speckle pattern were customized through the selective excitation of optical modes in the MMF. By applying singular vectors for mode modulation, the imaging quality can be effectively improved across four different types of scenes. Especially in the ultrafine 40 µm core MMF, the peak signal-to-noise ratio (PSNR) can be increased by up to 6.82 dB, and the structural similarity (SSIM) can be increased by up to 0.103, indicating a qualitative performance improvement of MMF imaging in minimally invasive medicine.

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“…Recently, researchers have developed novel techniques that utilize the deterministic nature of the multimode ber transmission matrix (TM) to perform bioimaging [30][31][32][33][34][35][36][37][38][39][40] . These approaches have enabled the acquisition of diffraction-limited images of uorescently labeled brain structures and neuronal activity, even in deep brain regions (e.g., head-xed mouse), using a multimode ber microendoscope 31 .…”

Section: Introductionmentioning

confidence: 99%

Demixing fluorescence time traces transmitted by multimode fibers

Rimoli,

Moretti,

Soldevila

et al. 2023

Preprint

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Fiber photometry is a significantly less invasive method compared to other deep brain imaging microendoscopy approaches due to the use of thin multimode fibers (MMF diameter < 500 µm). Nevertheless, the transmitted signals get scrambled upon propagation within the MMF, thus limiting the technique’s potential in resolving temporal readouts with cellular resolution. Here, we demonstrate how to separate the time trace signals of several fluorescent sources probed by a thin (≈ 200 µm) MMF with typical implantable length in a mouse brain. We disentangled several spatio-temporal fluorescence signals by using a general unconstrained non-negative matrix factorization (NMF) algorithm directly on the raw video data. Furthermore, we show that commercial and low-cost open-source miniscopes display enough sensitivity to image the same fluorescence patterns seen in our proof of principle experiment, suggesting that a whole new avenue for novel minimally invasive deep brain studies with multimode fibers in freely-behaving mice is possible.

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In Vivo Rat Brain Imaging through Full-Field Optical Coherence Microscopy Using an Ultrathin Short Multimode Fiber Probe

Cited by 12 publications

References 47 publications

Optical coherence tomography microscopy in experimental traumatic brain injury

Optical coherence tomography microscopy in experimental traumatic brain injury

Enhanced ultrafine multimode fiber imaging based on mode modulation through singular value decomposition

Demixing fluorescence time traces transmitted by multimode fibers

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