In vivo rapid fluorescence imaging of Alzheimer’s disease through accurate target bio-marking of zinc gluconate

In Vivo Biosynthesized Zinc and Iron Oxide Nanoclusters for High Spatiotemporal Dual-Modality Bioimaging of Alzheimer’s Disease

“…Common imaging technologies mainly include magnetic resonance imaging (MRI), computed tomography (CT), and positron emission tomography (PET). − With the well-known advantages including the high resolution of soft tissues, no radiation, and long-acting imaging, MRI is a kind of noninvasive method widely used in clinic diagnostics. , However, MRI is relatively high-cost and consumes large amounts of time, so real-time high-spatiotemporal fluorescence imaging (FL) has attracted much attention. In our previous studies, , we found that zinc gluconate solution could be readily utilized to realize fluorescence imaging of the lesion areas in the brains of Alzheimer’s model mice as a result of the obviously different redox environment and many metal ion distributions (e.g., zinc and iron) in AD brains compared to normal brains. To date, there has been little report on the combination of imaging for the diagnosis of Alzheimer’s disease.…”

“…15,16 However, MRI is relatively high-cost and consumes large amounts of time, so real-time high-spatiotemporal fluorescence imaging (FL) has attracted much attention. In our previous studies, 17,18 we found that zinc gluconate solution could be readily utilized to realize fluorescence imaging of the lesion areas in the brains of Alzheimer's model mice as a result of the obviously different redox environment and many metal ion…”

Tunable Nanomaterials of Intracellular Crystallization for In Situ Biolabeling and Biomedical Imaging

“…Subcutaneous injection of chloroauric acid solution into the vicinity of xenograft tumor in nude mice with hepatocellular carcinoma or chronic myeloid leukemia can effectively synthesize fluorescent gold nanoclusters without significantly diffusing to surrounding normal tissues, and then perform fluorescence imaging of tumor in situ . Alzheimer’s disease may be caused by the accumulation of amyloid protein, which may be the key protein for the synthesis of nanoclusters. , Taking pathological mice as the experimental model, it was found that zinc gluconate could pass through the blood–brain barrier and generate specific fluorescent zinc nanoclusters in the hippocampus . Schwartz-Duval’s research team also confirmed that mammalian cells can synthesize gold nanoparticles without adding chemicals. , In order to improve the synthesis efficiency of biosynthetic fluorescent nanoclusters, the synthesis mechanism of different metal elements in mammalian cells was studied.…”

Biomarker sensing platforms based on fluorescent metal nanoclusters

“…This method is especially interesting in the diagnosis of diseases that hold a striking histological feature, such as neurological diseases based on the deposition of amyloids. Lai et al 92 reported a biosensor to diagnose Alzheimer's disease (AD). Aβ42 is a peptide derivative of the amyloid precursor protein (APP), which is a large transmembrane protein that forms aggregates implicated in the initiation of the pathogenic cascade.…”