In vivo quantitation of tumour vascularisation assessed by Doppler sonography in rat mammary tumours

Denis, Fabrice; Bougnoux, Philippe; Poncheville, L. de; Prat, Marie; Catroux, Rodolphe; Tranquart, F.

doi:10.1016/s0301-5629(02)00478-7

Cited by 37 publications

(20 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This noninvasive technique offers many advantages, such as high sensitivity, simplicity of use, and repeatability (31). The recent development of high-frequency probes makes power Doppler sonography suitable for use in our rat model of chemically induced mammary tumors and provides a high reproducibility of vascular quantification in vivo (21). Using this approach to evaluate tumor vascularization, we found that tumor vascularization was lower in the group of rats supplemented with DHA than in the control group, even before anthracyclines treatment was initiated.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sensitization by Dietary Docosahexaenoic Acid of Rat Mammary Carcinoma to Anthracycline: A Role for Tumor Vascularization

Colas

Mahéo

Denis

et al. 2006

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

Purpose: To investigate whether dietary docosahexaenoic acid (DHA), a peroxidizable polyunsaturated N-3 fatty acids, sensitizes rat mammary tumors to anthracyclines and whether its action interferes with tumor vascularization, a critical determinant of tumor growth. Experimental Design: Female Sprague-Dawley rats were initiated by N-methylnitrosourea to develop mammary tumors and then assigned to a control group (n = 18), receiving a supplementation of palm oil, or to a DHA group (n = 54), supplemented with a microalgae-produced oil (DHASCO, 1.5 g/d). The DHA group was equally subdivided into three subgroups with addition of different amounts of a-tocopherol. Epirubicin was injected weekly during 6 weeks after the largest tumor reached 1.5 cm 2 , and subsequent changes in the tumor surface were evaluated. Tumor vascularization was assessed by power Doppler sonography before and during chemotherapy.Results: DHA and a-tocopherol were readily absorbed and incorporated into rat tissues. Epirubicin induced a 45% mammary tumor regression in the DHA-supplemented group, whereas no tumor regression was observed in the control group. In the DHA group, before chemotherapy was initiated, tumor vascular density was 43% lower than in the control group and remained lower during chemotherapy. Enhancement of epirubicin efficacy by DHA was abolished in a dosedependent manner by a-tocopherol, and the same trend was observed for DHA-induced reduction in tumor vascular density. Conclusions: Dietary DHA supplementation led to a reduction in tumor vascularization before the enhancement of any response to anthracyclines, suggesting that DHA chemosensitizes mammary tumors through an inhibition of the host vascular response to the tumor.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Gray scale and power Doppler sonography were done under anesthesia as already described (21). The duration of ultrasonography examination was limited to 30 minutes to prevent anesthesia-induced hypothermia.…”

Section: Methodsmentioning

confidence: 99%

Sensitization by Dietary Docosahexaenoic Acid of Rat Mammary Carcinoma to Anthracycline: A Role for Tumor Vascularization

Colas

Mahéo

Denis

et al. 2006

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…In studies using tumor-bearing mice, Doppler US has proved to be a valuable noninvasive method for serial examinations of the vascularity and perfusion within the tumor during antiangiogenic treatment and radiation therapy [20][21][22]. Power Doppler US records the amplitude (energy) of the Doppler signal reflected from the moving blood cells and was sensitive for detecting low velocities and parenchymal vessels as small as 15-20 μm in diameter [23].…”

Section: Discussionmentioning

confidence: 99%

Antitumor effects and blood flow dynamics after photodynamic therapy using benzoporphyrin derivative monoacid ring A in KLN205 and LM8 mouse tumor models

et al. 2007

View full text Add to dashboard Cite

1 SummaryPhotodynamic therapy (PDT) using benzoporphyrin derivative monoacid ring A (BPD-MA) induces direct tumor cell damage and microvascular injury. We administered BPD-MA at 3 h or 15 min before laser irradiation to KLN205 and LM8 tumors in murine models. Tumor growth delay was induced more effectively by 15-min-interval PDT than by 3-h-interval PDT. Vascularity and blood perfusion was significantly decreased by 15-min-interval PDT. We observed death of all tumor cells, except peripheral cells, in the 3-h-interval PDT group, and death of cells around the damaged tumor vasculature in the 15-min-interval PDT group. Thus, 15-min-interval-PDT enhanced the antitumor effect by damaging tumor vasculature.2

show abstract

“…The animals were placed in supine position and it was applied acoustic gel (Aquasonic  , Parker Laboratories Inc, Fairfield, New Jersey, USA), the ultrasonographic images using Power Doppler (PDI) and B Flow modes were obtained in sagittal planes using light pressure to avoid the distortion of tumors' shape. The images were recorded and the color pixels density (CPD) in PDI and B Flow images was determined according to the formula previously published by Denis et al [30], using Adobe Photoshop version 7.0.…”

Section: Ultrasonographic Evaluationmentioning

confidence: 99%

Long-term exercise training as a modulator of mammary cancer vascularization

Faustino-Rocha

Silva

Gabriel

et al. 2016

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

Background: Breast cancer remains a leading cause of death by cancer worldwide. It is commonly accepted that angiogenesis and the expression of angiogenic factors such as vascular endothelial growth factor-A (VEGF-A) is associated with the increased risk of metastasis and poor patient outcome.Objective: This work aimed to evaluate the effects of long-term exercise training on the growth and vascularization of mammary tumors in a rat model. Materials and methods:Fifty female Sprague-Dawley rats were divided into four groups: two N-methyl-N-nitrosourea (MNU)-exposed groups (exercised and sedentary) and two control groups (exercised and sedentary). MNU was administered once, intraperitoneally at 7 weeks-old. Animals were then exercised on a treadmill for 35 weeks. Mammary tumors were evaluated using thermography, ultrasonography [Power Doppler (PDI), B Flow and contrast-enhanced ultrasound (CEUS)], and immunohistochemistry (VEGF-A).Results: Both, MNU sedentary and exercised groups showed 100% of tumor incidence, but exercised animals showed less tumors with an increased latency period. Exercise training also enhanced VEGF-A immunoexpression and vascularization (microvessel density, MVD) (p < 0.05), and reduced histological aggressiveness. Ultrasound and 2 -thermal imaging analysis confirmed the enhanced vascularization of tumors on exercised animals.Conclusion: Long-term exercise training increased VEGF-A expression, leading to enhanced tumor vascularization and reduced tumor burden, multiplicity and histological aggressiveness.

show abstract

In vivo quantitation of tumour vascularisation assessed by Doppler sonography in rat mammary tumours

Cited by 37 publications

References 15 publications

Sensitization by Dietary Docosahexaenoic Acid of Rat Mammary Carcinoma to Anthracycline: A Role for Tumor Vascularization

Sensitization by Dietary Docosahexaenoic Acid of Rat Mammary Carcinoma to Anthracycline: A Role for Tumor Vascularization

Antitumor effects and blood flow dynamics after photodynamic therapy using benzoporphyrin derivative monoacid ring A in KLN205 and LM8 mouse tumor models

Long-term exercise training as a modulator of mammary cancer vascularization

Contact Info

Product

Resources

About