In vivo quantification of propylene glycol, glucose and glycerol diffusion in human skin with optical coherence tomography

Discrimination of dimethyl sulphoxide diffusion coefficient in the process of optical clearing by confocal micro-Raman spectroscopy

“…The percutaneous absorption dynamics had been studied by Fourier transform infrared spectroscopy (FTIR), optical coherence tomography, and second harmonic generation. [8][9][10] Unfortunately, these methods were not able to resolve the problem on diffusion coefficient change. In this article, we try to use confocal micro-Raman spectroscopy to solve this problem.…”

Effects of optical clearing agents on noninvasive blood glucose monitoring with optical coherence tomography: a pilot study

“…The room temperature was maintained at 25°C throughout the experiment, so as to eliminate influences caused by temperature fluctuation. 32,45 Four kinds of OCA were used in this study: 100% v∕v glycerol (100% G), 50% v∕v glycerol (50% G), 16% w∕v mannitol (16% M), and 16% w∕v mannitol (10% M) solutions. The 50% v∕v glycerol solution was prepared through mixing the agents with distilled water, and mannitol solutions were prepared by dissolving 16 or 10 g of mannitol in 100 mL purified and distilled water.…”

“…In this study, OCT images obtained from human skin were processed using an original program developed in Microsoft Visual C++ 6.0 to obtain the OCT signal and calculate the OCT signal slope (OCTSS) 45 Over approximately 1 mm, which is sufficient for speckle noise suppression, the 2-D images were averaged in the lateral (x axis) direction into a single curve to obtain an OCT signal that represents 1-D distribution of light in depth. 26 To reduce the effect of surface curvature, the most flat regions of the 2-D images were used for averaging.…”

“…26 To reduce the effect of surface curvature, the most flat regions of the 2-D images were used for averaging. 45 The 1-D distribution was plotted in logarithmic scale as a function of depth for further analysis.…”

Collaborative effects of wavefront shaping and optical clearing agent in optical coherence tomography

“…In fact, various mechanisms are involved, such as the dissociation of collagen 15,16 , the ability to disrupt the hydration shell and the water-mediated hydrogen bonds of collagen molecules, 17 and the dehydration process 10,[18][19][20] . In addition to penetration depth enhancement for tissue imaging [21][22][23] , OCAs also enable optical blood clearing 24 and monitoring of molecular diffusion [25][26][27][28][29][30] using OCT systems. Whereas OCA approaches use the scattering properties of tissue samples for the enhancement of the penetration depth, it has been recently demonstrated that controlling the illumination light in optical imaging can offer a high possibility of overcoming multiple scattering without affecting the sample.…”