In vivo pulse labeling of isochronic cohorts of cells in the
              central nervous system using FlashTag

Govindan, Subashika; Oberst, Polina; Jabaudon, Denis

doi:10.1038/s41596-018-0038-1

Cited by 64 publications

(81 citation statements)

References 26 publications

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“…FlashTag (FT) injections were performed at E12, E13, E14 or E15, as previously described (Telley et al 2016;Govindan et al 2018). Briefly, pregnant females were anaesthetized with isoflurane, treated with Temgesic (Reckitt Benckiser, Switzerland) and the uterine horn was exposed following an abdominal incision.…”

Section: In Utero Flashtag Injectionmentioning

confidence: 99%

“…Cell dissociation and FAC-sorting: Pregnant females were sacrificed either 1, 24 or 96 hours after FT injection. As previously described (Telley et al 2016;Govindan et al 2018), embryonic brains were extracted in ice-cold HBSS, embedded in 4% agar low-melt and sectioned coronally at 300 μm using a vibrating microtome (Leica, #VT100S). The putative S1 cortical region was microdissected under a stereomicroscope and incubated in 0.05% trypsin at 37°C for 5 minutes.…”

Section: Scrnaseq Experimentsmentioning

confidence: 99%

“…To address these questions, we used FlashTag (FT), a high temporal resolution method to pulse-label APs and their daughter neurons (Telley et al 2016;Govindan et al 2018), to trace the transcriptional trajectories of successive generations of APs and isochronic cohorts of their daughter neurons between embryonic day (E) 12 and E15. This corresponds to the period during which APs successively generate layer (L) 6, L5, L4 and L2/3 neurons (Jabaudon 2017).…”

mentioning

confidence: 99%

“…1A and fig. S1, A and B) (Telley et al 2016;Govindan et al 2018). We performed single-cell RNA sequencing at each of these 3 differentiation stages and 4 embryonic ages (E12, E13, E14, and E15), which yielded a total of 2,756 quality-controlled cells across 12 conditions for analysis (fig.…”

mentioning

confidence: 99%

“…1C and fig. S2B), likely corresponding to cells migrating into the dorsal pallium after FT labeling of their progenitors in the ventral pallium (Govindan et al 2018;Wamsley & Fishell 2017;Marin 2013). Astrocytes, corresponding to 4-day-old daughter cells of E15 APs (Minocha et al 2017;Cahoy et al 2008) were also present ( Fig.…”

mentioning

confidence: 99%

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Single-cell transcriptional dynamics and origins of neuronal diversity in the developing mouse neocortex

Telley

Agirman

Prados

et al. 2018

Preprint

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D During cortical development, distinct subtypes of glutamatergic neurons are sequentially born and differentiate from dynamic populations of progenitors. The neurogenic competence of these progenitors progresses as corticogenesis proceeds; likewise, newborn neurons transit through sequential states as they differentiate. Here, we trace the developmental transcriptional trajectories of successive generations of apical progenitors (APs) and isochronic cohorts of their daughter neurons using parallel single-cell RNA sequencing between embryonic day (E) 12 and E15 in the mouse cerebral cortex. Our results identify the birthdate-and differentiation stagerelated transcriptional dynamics at play during corticogenesis. As corticogenesis proceeds, APs transit through embryonic age-dependent molecular states, which are transmitted to their progeny to generate successive initial daughter cell identities. In neurons, essentially conserved post-mitotic differentiation programs are applied onto these distinct AP-derived ground states, allowing temporally-regulated sequential emergence of specialized neuronal cell types. Molecular temporal patterning of sequentially-born daughter neurons by their respective mother cell thus underlies emergence of neuronal diversity in the neocortex. One Sentence Summary: During corticogenesis, temporally dynamic molecular birthmarks are transmitted from progenitors to their post-mitotic progeny to generate neuronal diversity.

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Section: In Utero Flashtag Injectionmentioning

confidence: 99%

Section: Scrnaseq Experimentsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Single-cell transcriptional dynamics and origins of neuronal diversity in the developing mouse neocortex

Telley

Agirman

Prados

et al. 2018

Preprint

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The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors

Lozano Casasbuenas,

Kortebi,

Gora

et al. 2024

Glia

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Astrocytes that reside in superficial (SL) and deep cortical layers have distinct molecular profiles and morphologies, which may underlie specific functions. Here, we demonstrate that the production of SL and deep layer (DL) astrocyte populations from neural progenitor cells in the mouse is temporally regulated. Lineage tracking following in utero and postnatal electroporation with PiggyBac (PB) EGFP and birth dating with EdU and FlashTag, showed that apical progenitors produce astrocytes during late embryogenesis (E16.5) that are biased to the SL, while postnatally labeled (P0) astrocytes are biased to the DL. In contrast, astrocytes born during the predominantly neurogenic window (E14.5) showed a random distribution in the SL and DL. Of interest, E13.5 astrocytes birth dated at E13.5 with EdU showed a lower layer bias, while FT labeling of apical progenitors showed no bias. Finally, examination of the morphologies of “biased” E16.5‐ and P0‐labeled astrocytes demonstrated that E16.5‐labeled astrocytes exhibit different morphologies in different layers, while P0‐labeled astrocytes do not. Differences based on time of birth are also observed in the molecular profiles of E16.5 versus P0‐labeled astrocytes. Altogether, these results suggest that the morphological, molecular, and positional diversity of cortical astrocytes is related to their time of birth from ventricular/subventricular zone progenitors.

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Contributions of Single-Cell Approaches for Probing Heterogeneity and Dynamics of Neural Progenitors Throughout Life: Concise Review

Marcy

Raineteau

2019

Stem Cells

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Development of the forebrain occurs in a stepwise manner from a pool of neural progenitors (NPs), which differs over space and time to produce distinct progenies. The sequence of events leading to the generation of the exquisite complexity of cell types that compose this tissue has been described in great detail at the population level. Recent advances in histology and transcriptomics have allowed probing spatial and temporal heterogeneity and dynamics of NPs at the single-cell level. Clonal fate mapping studies highlight a deterministic behavior as well as the existence of trajectories in the lineage progression of prenatal and postnatal NPs, whereas single-cell transcriptomic studies shed new light on the transcriptional signatures of these processes. Here, we review this recent work and integrate it to our current understanding of forebrain germinal activity at prenatal and postnatal time points. STEM CELLS 2019;37:1381-1388 SIGNIFICANCE STATEMENTClonal analysis in histology and single-cell transcriptomics studies have recently shed new light on the understanding of forebrain germinal activity at prenatal and postnatal time points. This article discusses recent contributions of these approaches in probing lineage progression and competence of neural progenitors.

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In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag

Cited by 64 publications

References 26 publications

Single-cell transcriptional dynamics and origins of neuronal diversity in the developing mouse neocortex

Single-cell transcriptional dynamics and origins of neuronal diversity in the developing mouse neocortex

The laminar position, morphology, and gene expression profiles of cortical astrocytes are influenced by time of birth from ventricular/subventricular progenitors

Contributions of Single-Cell Approaches for Probing Heterogeneity and Dynamics of Neural Progenitors Throughout Life: Concise Review

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