In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing

Nuutila, Kristo; Samandari, Mohamadmahdi; Endo, Yori; Zhang, Yuteng; Quint, Jacob; Schmidt, Tannin A.; Tamayol, Ali; Sinha, Indranil

doi:10.1016/j.bioactmat.2021.06.030

Cited by 81 publications

(73 citation statements)

References 73 publications

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“…As direct in vivo printing of scaffolds has drawn significant attention recently, the suitability of the engineered foam bioink for in vivo printing applications was explored using a partially automated custom-built handheld printer 40,45,46 [Figs. 3(d)-3(i)].…”

Section: Characterization Of the Rheological Properties And The Print...mentioning

confidence: 99%

Colloidal multiscale porous adhesive (bio)inks facilitate scaffold integration

Mostafavi

Samandari

Karvar

et al. 2021

Applied Physics Reviews

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Poor cellular spreading, proliferation, and infiltration, due to the dense biomaterial networks, have limited the success of most thick hydrogel-based scaffolds for tissue regeneration. Here, inspired by whipped cream production widely used in pastries, hydrogel-based foam bioinks are developed for bioprinting of scaffolds. Upon cross-linking, a multiscale and interconnected porous structure, with pores ranging from few to several hundreds of micrometers, is formed within the printed constructs. The effect of the process parameters on the pore size distribution and mechanical and rheological properties of the bioinks is determined. The developed foam bioinks can be easily printed using both conventional and custom-built handheld bioprinters. In addition, the foam inks are adhesive upon in situ cross-linking and are biocompatible. The subcutaneous implantation of scaffolds formed from the engineered foam bioinks showed their rapid integration and vascularization in comparison with their non-porous hydrogel counterparts. In addition, in vivo application of the foam bioink into the non-healing muscle defect of a murine model of volumetric muscle loss resulted in a significant functional recovery and higher muscle forces at 8 weeks post injury compared with non-treated controls.

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Section: Characterization Of the Rheological Properties And The Print...mentioning

confidence: 99%

Colloidal multiscale porous adhesive (bio)inks facilitate scaffold integration

Mostafavi

Samandari

Karvar

et al. 2021

Applied Physics Reviews

Self Cite

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“…In particular, membranous ECM-based scaffolds, such as acellular dermal matrix or small intestinal submucosa, were loaded with antibacterial nanoparticles, antiseptic peptides, or anti-inflammatory agents or growth factors (GFs) [ 33 ]. For example, bFGF (basic fibroblast growth factor) or VEGF was added to some dressings in order to reinforce granulation-tissue formation in the initial healing phase [ 34 ]; specifically, porcine decellularized adipose tissue loaded with VEGF showed enhanced angiogenesis in vivo and in vitro [ 35 ]. Likewise, the incorporation of rhEGF (recombinant human epidermal growth factor) or rhPDGF was used to stimulate cell proliferation and growth in a subsequent healing phase [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, analyses of in vivo biodegradability and the rate of wound healing are not addressed here; these are major limitations that do not allow us to determine the true value of our bioprinted constructs in wound repair. Our future studies will utilize these combinational bioinks to investigate the in situ delivery of advanced dressings with handheld devices [ 34 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting

Amo

Argimiro

Cascajo

et al. 2022

IJMS

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In patients with comorbidities, a large number of wounds become chronic, representing an overwhelming economic burden for healthcare systems. Engineering the microenvironment is a paramount trend to activate cells and burst-healing mechanisms. The extrusion bioprinting of advanced dressings was performed with novel composite bioinks made by blending adipose decellularized extracellular matrix with plasma and human dermal fibroblasts. Rheological and microstructural assessments of the composite hydrogels supported post-printing cell viability and proliferation over time. Embedded fibroblasts expressed steady concentrations of extracellular matrix proteins, including type 1, 3 and 4 collagens and fibronectin. ELISA assessments, multiplex protein arrays and ensuing bioinformatic analyses revealed paracrine activities corresponding to wound-healing activation through the modulation of inflammation and angiogenesis. The two modalities of advanced dressings, differing in platelet number, showed differences in the release of inflammatory and angiogenic cytokines, including interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The conditioned media stimulated human-dermal-cell proliferation over time. Our findings open the door to engineering the microenvironment as a strategy to enhance healing.

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“…In VEGF-loaded GelMA, it may be adequate to have GelMA physically trap VEGF without covalent bonding between them. Recently, Nuutila and Samandari et al [23] invented an in situ hand-held VEGF-GelMA printer. VEGF was not found to be covalently bonded to GelMA.…”

Section: Vegfmentioning

confidence: 99%

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

et al. 2022

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To recreate or substitute tissue in vivo is a complicated endeavor that requires biomaterials that can mimic the natural tissue environment. Gelatin methacrylate (GelMA) is created through covalent bonding of naturally derived polymer gelatin and methacrylic groups. Due to its biocompatibility, GelMA receives a lot of attention in the tissue engineering research field. Additionally, GelMA has versatile physical properties that allow a broad range of modifications to enhance the interaction between the material and the cells. In this review, we look at recent modifications of GelMA with naturally derived polymers, nanomaterials, and growth factors, focusing on recent developments for vascular tissue engineering and wound healing applications. Compared to polymers and nanoparticles, the modifications that embed growth factors show better mechanical properties and better cell migration, stimulating vascular development and a structure comparable to the natural-extracellular matrix.

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In vivo printing of growth factor-eluting adhesive scaffolds improves wound healing

Cited by 81 publications

References 73 publications

Colloidal multiscale porous adhesive (bio)inks facilitate scaffold integration

Colloidal multiscale porous adhesive (bio)inks facilitate scaffold integration

Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting

Gelatin Methacrylate Hydrogel for Tissue Engineering Applications—A Review on Material Modifications

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