In vivo platelet response to lipopolysaccharide in mice: proposed method for evaluating new antiplatelet drugs

Hepatic platelet accumulation in Fas-mediated hepatitis in mice

“…Our method (a) provides information concerning intact platelets because it needs neither artificial treatment of platelets (such as labeling after separation from blood) nor anesthesia of the animal, and (b) relies on the fact that the 5HT present in the blood and tissues (except brain and gastrointestinal tract) is largely contained within platelets. We have repeatedly confirmed that our method is useful for the analysis of in vivo platelet behaviors [4,7,9,10,11,22]. In most sections in the text, the 5HT increase in the liver is referred to as HPA for convenience, with the degree of HPA being expressed in terms of the amount of 5HT present in one gram of liver (nmol/g).…”

“…HPA was estimated as described previously, using 5HT as a marker for platelets [22]. Briefly, after collecting blood for the measurement of serum aminotransferase activities or platelet count (see above), an additional one or two drops of blood from the same mouse were collected into another pre-weighed tube in which was 3 ml of 0.4 M HClO 4 containing 0.2% N-acetylcysteine-HCl and 4 mM EDTA-2Na.…”

Serotonin activates murine alveolar macrophages through 5-HT2Creceptors

“…Serotonin levels are elevated in inflamed tissues (36), and they reach 50 -60 nmol/g, corresponding to ϳ5 ϫ 10 Ϫ5 M in mouse lung homogenates within minutes after LPS injection or antigen challenge in sensitized animals (41,56). However, these data do not discriminate between intracellular and free 5-HT.…”

Pharmacological characterization of anaphylaxis-like shock responses induced in mice by mannan and lipopolysaccharide

“…The required procedures were carried out as previously described [12,32,39]. Two or three drops of blood from each decapitated mouse were directly collected into a test tube for the measurement of platelet count or 5HT.…”