In vivo plasmid DNA electroporation resulted in transfection of satellite cells and lasting transgene expression in regenerated muscle fibers

Peng, Bowen; Zhao, Yuwu; Lu, Huili; Pang, Wengka; Xu, Yuhong

doi:10.1016/j.bbrc.2005.10.111

Cited by 41 publications

(29 citation statements)

References 38 publications

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“…Electroporation to skeletal muscle induces regenerative response [5,37]. Several experimental models for the study of skeletal muscle regeneration have been established so far, and BPVC injection into skeletal muscle is one of them [46].…”

Section: Discussionmentioning

confidence: 99%

“…Electroporation is known to induce degenerative/regenerative response of skeletal muscle [5,37]. To examine the electroporation-induced degenerative/regenerative response in skeletal muscle, histological analyses were performed on rat TA muscles after electroporation with plasmid DNA (pCAGGS-lacZ).…”

Section: Electroporation Induces Degenerative/regenerative Response Imentioning

confidence: 99%

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<i>In Vivo</i> Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle

et al. 2012

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ABSTRACT. Adult urodele amphibians such as newts are capable of regenerating lost structures including their limbs. In these species, dedifferentiation of myofiber is essential for the regenerative process. Upon terminal differentiation, nuclei of myofiber (myonuclei) are withdrawn from cell cycle, but prior to dedifferentiation, myonuclei reenter the cell cycle. In contrast with urodele amphibians, it is generally accepted that mammalian myofibers are not able to dedifferentiate in response to muscle injury. A recent study has suggested that electroporation can induce dedifferentiation response of skeletal muscle in newt limbs. In the present study, we examined whether myonuclei of skeletal muscle of mammals are capable of reentering the cell cycle by means of electroporation. Electroporation was applied to tibialis anterior muscle of the rat with or without plasmid DNA. Histological analyses revealed that, while electroporation induces degenerative/regenerative responses in skeletal muscle irrespective of the presence of plasmid DNA, the expression of proliferating cell nuclear antigen (PCNA) in myonuclei was observed only in the presence of plasmid DNA. The present results indicate that myonuclei of skeletal muscle are capable of reentering the cell cycle and suggest that in vivo electroporation can induce dedifferentiation of mammalian skeletal muscle.

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Section: Discussionmentioning

confidence: 99%

Section: Electroporation Induces Degenerative/regenerative Response Imentioning

confidence: 99%

<i>In Vivo</i> Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle

et al. 2012

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“…These satellite cells replace the damaged muscular cells. Their transfection seems important in order to have a long expression of the transgene (Peng et al, 2005). Besides the role of the pulse on the efficiency, let's not forget the electrodes configuration and composition.…”

Section: Electrotransfermentioning

confidence: 99%

Gene Therapy Challenges in Arthritis

Denys¹,

Thiolat²,

Marie-Christophe³

et al. 2011

Gene Therapy Applications

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“…Electroporation of DNA into skeletal muscle can transfect satellite cells: Electroporation of plasmid DNA into skeletal muscle has been shown to transfect satellite cells with exogenously introduced gene [11]. In order to determine if electroporated plasmid DNA is indeed introduced into satellite cells, frozen sections of rat TA muscle on day 7 after electroporation of pCXN2-EGFP were immunostained with anti-desmin [1], and co-localization of EGFP and desmin was examined.…”

Section: Histological Analyses Of Skeletal Muscle After Electroporationmentioning

confidence: 99%

“…Introduction of genes of osteogenic growth factor, bone morphogenic protein (BMP)-2 and -7, into skeletal muscle by electroporation has been shown to induce ectopic bone formation [9], suggesting that the presence of cells with osteogenicity and the appropriate niches are required for them to terminally differentiate to osteocytes. Recent study indicated that electroporation induces degenerative/regenerative response of skeletal muscle, thus leading to an activation of satellite cells, and can be used not only to introduce genes of interest into skeletal muscle fibres but also to transfect satellite cells in vivo [11].…”

mentioning

confidence: 99%

In Vivo Gene Transfer of PPAR.GAMMA. Is Insulfficient to Induce Adipogenesis in Skeletal Muscle

Ban

Yamanouchi

Matsuwaki

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Skeletal muscle contains several progenitor/stem cells with myogenicity as well as adipogenicity such as satellite cells. Our previous study demonstrated that forced expression of PPARγ is sufficient to induce transdifferentiation of predetermined myoblasts in vitro. In the present study, we examined whether introduction of PPARγ gene could induce adipogenesis of satellite cells in vivo. A plasmid vector containing enhanced green fluorescent protein (EGFP) or PPARγ gene was introduced into rat tibialis anterior muscle by electroporation. Histological analyses revealed that electroporation induces degenerative/regenerative response in skeletal muscle, including activation of satellite cells. When EGFP gene was introduced, newly formed myotubes resulted from fusion of activated satellite cells, showed EGFP expression, indicating that electroporation could transfect satellite cells with exogenously introduced gene. Gene transfer of PPARγ resulted in an increase of PPARγ-positive mononucleated cells on day 3 after electroporation but failed to induce adipogenesis thereafter. These results suggested that, in addition to an expression of PPARγ, niches that support adipogenesis are required for satellite cells to enter adipogenesis in vivo.

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In vivo plasmid DNA electroporation resulted in transfection of satellite cells and lasting transgene expression in regenerated muscle fibers

Cited by 41 publications

References 38 publications

<i>In Vivo</i> Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle

<i>In Vivo</i> Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle

Gene Therapy Challenges in Arthritis

In Vivo Gene Transfer of PPAR.GAMMA. Is Insulfficient to Induce Adipogenesis in Skeletal Muscle

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