In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft

Zhou, Quan; Zhao, Li; Zhou, Juan; Joshi, Bishnu P.; Li, Gaoming; Duan, Xiyu; Kuick, Rork; Owens, Scott; Wang, Thomas D.

doi:10.1016/j.pacs.2016.04.001

Cited by 14 publications

(20 citation statements)

References 102 publications

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“…However, our data provided the first evidence that EGFR also acted as a target of miR‐195. EGFR pathway plays an essential role in cell proliferation, survival, and migration and its altered activity has been implicated in the development and growth of many tumors including HCC . This was also confirmed in our study that EGFR served as an oncogene in HCC.…”

Section: Discussionsupporting

confidence: 89%

Retracted: Knockdown of long non‐coding RNA MALAT1 inhibits growth and motility of human hepatoma cells via modulation of miR‐195

Liu¹,

Zhu²,

Pang³

et al. 2017

J of Cellular Biochemistry

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The metastasis-associated lung adenocarcinoma transcription 1 (Malat1) is a long non-coding RNA (lncRNA), exerts oncogenic role in multiple cancers, including hepatocellular carcinoma (HCC). This study was aimed to investigate its posttranscriptional regulation in HCC cells. RT-PCR was performed to monitor the expression levels of Malat1 in normal liver and HCC cell lines. The expression of Malat1, microRNA (miR)-195, and epidermal growth factor receptor (EGFR) in HepG2 and MHCC97 cells was respectively or synchronously altered by transfection. Then the changes in cell viability, apoptotic cell rate, cell cycle distribution, migration, and invasion were respectively assessed. As a result, we found that Malat1 was highly expressed in HCC cell lines when compared to normal liver cells. Malat1 silence suppressed HCC cells viability, migration and invasion, induced apoptosis, and arrested more cells in G0/G1 phase. Malat1 acted as a circular endogenous RNA (ceRNA) for miR-195. Malat1 silence could not suppress HCC cell growth and motility when miR-195 was knocked down. EGFR was a direct target of miR-195. miR-195 overexpression could not suppress HCC cell growth and motility when the 3'UTR site of EGFR was overexpressed. Furthermore, Malat1 silence blocked the activation of PI3K/AKT and JAK/STAT pathways, while EGFR overexpression activated them. Our study demonstrates Malat1-miR-195-EGFR axis plays a critical role in HCC cells which provided a better understanding of Malat1 in HCC.

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Section: Discussionsupporting

confidence: 89%

Retracted: Knockdown of long non‐coding RNA MALAT1 inhibits growth and motility of human hepatoma cells via modulation of miR‐195

Liu¹,

Zhu²,

Pang³

et al. 2017

J of Cellular Biochemistry

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“…Similarly, EGFR is a relevant biomarker of aggressiveness and resistance to therapy, highly expressed in many types of cancer, and therefore, it appears as a promising target for in vivo imaging. Zhou et al [42] have investigated the use of an EGFR-specific peptide bonded to Cy5.5, a cyanine dye with spectral features similar to ICG and favorable pharmacokinetic and labeling properties, to detect HCC in a preclinical model by multimodal US-MR-PA imaging. PAI added relevant in vivo molecular information to the US and MRI characterization of tumor growth, as confirmed by immunofluorescence and immunohistochemistry.…”

Section: Biomedical Pai Applications Based On Exogenous Optical Comentioning

confidence: 99%

State-of-the-Art Preclinical Photoacoustic Imaging in Oncology: Recent Advances in Cancer Theranostics

Gargiulo

Albanese

Mancini

2019

Contrast Media & Molecular Imaging

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The optical imaging plays an increasing role in preclinical studies, particularly in cancer biology. The combined ultrasound and optical imaging, named photoacoustic imaging (PAI), is an emerging hybrid technique for real-time molecular imaging in preclinical research and recently expanding into clinical setting. PAI can be performed using endogenous contrast, particularly from oxygenated and deoxygenated hemoglobin and melanin, or exogenous contrast agents, sometimes targeted for specific biomarkers, providing comprehensive morphofunctional and molecular information on tumor microenvironment. Overall, PAI has revealed notable opportunities to improve knowledge on tumor pathophysiology and on the biological mechanisms underlying therapy. The aim of this review is to introduce the principles of PAI and to provide a brief overview of current PAI applications in preclinical research, highlighting also on recent advances in clinical translation for cancer diagnosis, staging, and therapy.

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“…The T/B ratio was significantly different between the EGFR and control peptide. The signal was diminished by 24 hours [43]. From the data, a peptide specific for EGFR can detect HCC xenograft tumors in vivo with photoacoustic imaging.…”

Section: Photoacoustic Tomographymentioning

confidence: 89%

Targeting Peptides Derived from Phage Display for Clinical Imaging

Khondee¹,

Piyawattanametha²

2020

Bacteriophages - Perspectives and Future

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Phage display is a high-throughput technology used to identify peptides or proteins with high and specific binding affinities to a target, which is usually a protein biomarker or therapeutic receptor. In general, this technique allows peptides with a particular sequence to be presented on a phage particle. Peptides derived from phage display play an important role in drug discovery, drug delivery, cancer imaging, and treatment. Phage peptides themselves can act as sole therapeutics, for example, drugs, gene therapeutic, and immunotherapeutic agents that are comprehensively described elsewhere. In this chapter, we discuss phage selection and screening procedures in detail including some modifications to reduce nonspecific binding. In addition, the rationale for discovery and utilization of phage peptides as molecular imaging probes is focused upon. Molecular imaging is a new paradigm that uses advanced imaging instruments integrated with specific molecular imaging probes. Applications include monitoring of metabolic and molecular functions, therapeutic response, and drug efficacy, as well as early cancer detection, personalized medicine, and image-guided therapy.

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In vivo photoacoustic tomography of EGFR overexpressed in hepatocellular carcinoma mouse xenograft

Cited by 14 publications

References 102 publications

Retracted: Knockdown of long non‐coding RNA MALAT1 inhibits growth and motility of human hepatoma cells via modulation of miR‐195

Retracted: Knockdown of long non‐coding RNA MALAT1 inhibits growth and motility of human hepatoma cells via modulation of miR‐195

State-of-the-Art Preclinical Photoacoustic Imaging in Oncology: Recent Advances in Cancer Theranostics

Targeting Peptides Derived from Phage Display for Clinical Imaging

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