In Vivo Phosphorescence Imaging of pO2 Using Planar Oxygen Sensors

Babilas, Philipp; Liebsch, Gregor; Schacht, Vivien; Klimant, Ingo; Wolfbeis, Otto S.; Szeimies, Rolf‐Markus; Abels, Christoph

doi:10.1080/10739680591003314

Cited by 72 publications

(69 citation statements)

References 48 publications

(84 reference statements)

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“…The outline of the tumor (black) is superimposed on the pO 2 maps. The pO 2 maps from data acquired 3,8,19, and 78 minutes after administration of nicotinamide are shown in Fig. 3(c)-3(f).…”

Section: Modulation With Nicotinamidementioning

confidence: 99%

“…Conversion between phosphorescent lifetime values and pO 2 was based on a two point calibration procedure, since previous characterization of these coatings reported a linear response [8]. A linear calibration fit was made for the lifetimes of two measurements, one with exposure of the coverslip to air (pO 2 = 140 mmHg) and the other with exposure of the coverslip to a zero dissolved oxygen solution (Rica Chemical Company, Arlington, TX), containing sodium sulfite (pO 2 = 0 mmHg).…”

Section: Phosphorescent Lifetime Instrumentation and Imaging Proceduresmentioning

confidence: 99%

“…Babilas et al have shown the ability to encapsulate platinum porphyrin molecules in a polymer matrix and coat it on the surface of a coverslip. The group demonstrated the approach using a titanium dorsal skin-fold window chamber hamster model for single baseline measurements [8]. This approach has potential to be utilized for repeatable measurements over the course of a week without the need for additional introduction of new probe.…”

Section: Introductionmentioning

confidence: 99%

“…One approach has been the utilization of porphyrin molecules that change the rate of phosphorescence emission (lifetime), dependent on the amount of oxygen present. Oxygen acts as a quencher that decreases the phosphorescence lifetime [8]. Most in vivo studies have utilized intravascular probes, although there has been some development of probes measuring extracellular oxygenation outside of the vascular system [9].…”

Section: Introductionmentioning

confidence: 99%

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Dynamic oxygenation measurements using a phosphorescent coating within a mammary window chamber mouse model

Schafer

Gmitro

2015

Biomed. Opt. Express

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Phosphorescent lifetime imaging was employed to measure the spatial and temporal distribution of oxygen partial pressure in tissue under the coverslip of a mammary window chamber breast cancer mouse model. A thin platinum-porphyrin coating, whose phosphorescent lifetime varies monotonically with oxygen partial pressure, was applied to the coverslip surface. Dynamic temporal responses to induced modulations in oxygenation levels were measured using this approach.

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Section: Modulation With Nicotinamidementioning

confidence: 99%

Section: Phosphorescent Lifetime Instrumentation and Imaging Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dynamic oxygenation measurements using a phosphorescent coating within a mammary window chamber mouse model

Schafer

Gmitro

2015

Biomed. Opt. Express

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“…For example, documenting the oxygen dynamics during cell infection and cancerogenesis could help identify specific drug targets to slow or stop the uncontrolled growth of cancer cells (Babilas et al, 2005). Currently, planar sensors have been developed to specifically detect oxygen, carbon dioxide or pH.…”

Section: Prospects For Planar Oxygen Sensingmentioning

confidence: 99%

Planar Oxygen Sensors for Non Invasive Imaging in Experimental Biology

Tschiersch¹,

Liebsch²,

Stangelmayer³

et al. 2011

Microsensors

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Sensor Paints

Wolfbeis

2008

Advanced Materials

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116

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Fluorescence microscopy is widely used for chemical imaging of intrinsically fluorescent chemical species, such as chlorophylls, or nonfluorescent species (e.g., DNA) that have been labeled appropriately or to which a relevant molecular probe has been added. However, there are numerous analytes that neither have an intrinsic luminescence nor can be rendered luminescent with the help of labels or probes. Such parameters include oxygen, pH, CO2, ammonia, and glucose. There are also numerous applications where the 2D distribution of a chemical or physical parameter is of interest. The use of a “sensor paint” is ideal in such situations. The state of this new technology is discussed herein with emphasis on current and future trends, for example, the use of pressure‐ and temperature‐sensitive paints; known technological limitations are also discussed. These “paints” respond to a (bio)chemical parameter with a change in their optical properties. The object of interest is painted and the color or fluorescence of the paint is monitored by optical imaging. This technique enables monitoring of physical and chemical parameters, over relatively large areas and in real time.

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In Vivo Phosphorescence Imaging of pO₂ Using Planar Oxygen Sensors

Abstract: Using the presented method the surface pO(2) distribution can be mapped with a high temporal resolution of approximately 100 ms and a spatial resolution of at least 25 mu m. Moreover, the transparent sensor allows the simultaneous visualization of the underlying microvasculature.

Cited by 72 publications

References 48 publications

Dynamic oxygenation measurements using a phosphorescent coating within a mammary window chamber mouse model

Dynamic oxygenation measurements using a phosphorescent coating within a mammary window chamber mouse model

Planar Oxygen Sensors for Non Invasive Imaging in Experimental Biology

Sensor Paints

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