In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary gland

Gajewska, Alina; Herman, Andrzej Przemysław; Woliñska-Witort, Ewa; Kochman, Kazimierz; Zwierzchowski, L.

doi:10.1530/jme-14-0092

Cited by 7 publications

(3 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been demonstrated that the level of PITX1 is correlated with patient survival; patients with higher PITX1 levels exhibit a longer median overall survival in human osteosarcoma (24). The reduced expression of PITX1 is also independently correlated with shorter patient survival and may serve as a prognostic marker in colorectal carcinoma (32). In a previous study (29), it was revealed that the expression of PITX1 is decreased in GC tissues and that the increased expression of PITX1 significantly suppresses the proliferation of GC cells and tumorigenesis in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

Silenced PITX1 promotes chemotherapeutic resistance to 5‑fluorocytosine and cisplatin in gastric cancer cells

Shen

et al. 2019

Exp Ther Med

View full text Add to dashboard Cite

Resistance to chemotherapeutic drugs leads to a poor prognosis in gastric cancer (GC). The present study aimed to assess the association between pituitary homeobox paired homeodomain transcription 1 (PITX1) expression and the sensitivity of GC cells to the chemotherapeutic drugs 5-fluorouracil (5-FU) and cisplatin (CDDP). In the present study, the gastric cancer cell lines GES-1, AGS, BGC-823, MCG-803 and SGC-7901 were used. The expression of PITX1 was determined via reverse transcription-quantitative polymerase chain reaction in GC cell lines. AGS and BGC-823 cells, which exhibit a decreased PITX1 expression, were transfected with a PITX1 cDNA construct and its control vector. MCG-803 and SGC-7901 cells, which exhibit an increased PITX1 expression, were transfected with siRNA against PITX1 and its control scramble sequence. A Cell Counting kit-8 assay was performed to determine the impact of PITX1 expression on the sensitivity of GC cells to 5-FU and CDDP. The Cancer Genome Atlas database was used to analyze the expression of PITX1 with GC prognosis in the Asian population and to assess the potential mechanism of PITX1 in 5-FU and CDDP resistance. The results revealed that the overexpression of PIXT1 increased the sensitivity of GC cells to 5-FU/CDDP. The combination of 5-FU/CDDP and PITX1 overexpression also reduced the proliferation of GC cells. Additionally, PIXT1 knockdown decreased the sensitivity of GC cells to 5-FU/CDDP. TCGA data revealed that a lower expression of PITX1 is exhibited in Asian GC patients than in normal individuals. GC patients with a lower expression of PITX1 had a poor prognosis. The expression of PITX1 affected the sensitivity of GC cells to 5-FU/CDDP, indicating that PITX1 may increase the efficacy of treatment in GC patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Silenced PITX1 promotes chemotherapeutic resistance to 5‑fluorocytosine and cisplatin in gastric cancer cells

Shen

et al. 2019

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…We also found that lncRNA MSTRG.8143.5, which was upregulated at the follicular phase, simultaneously targeted DLL1 , EGR1 , GH1 , DUSP1 and NTRK2 in the sheep pituitary gland. The early-growth-response ( EGR1 ) gene resides within the GnRH transcriptional network and belongs to the primary-response-gene family [ 43 ]. The detectable changes in EGR1 transcription occur as early as possible within 1 h of GnRH stimulation [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Hu Sheep Pituitary Gland Prolificacy at the Follicular and Luteal Phases

Wan

Yang

Cai

et al. 2022

Genes

View full text Add to dashboard Cite

The pituitary gland directly regulates the reproduction of domestic animals. Research has increasingly focused on the potential regulatory mechanism of non-coding RNA in pituitary development. Little is known about the differential expression pattern of lncRNAs in Hu sheep, a famous sheep breed with high fecundity, and its role in the pituitary gland between the follicular phase and luteal phase. Herein, to identify the transcriptomic differences of the sheep pituitary gland during the estrus cycle, RNA sequencing (RNA-Seq) was performed. The results showed that 3529 lncRNAs and 16,651 mRNAs were identified in the pituitary gland. Among of them, 144 differentially expressed (DE) lncRNA transcripts and 557 DE mRNA transcripts were screened in the follicular and luteal phases. Moreover, GO and KEGG analyses demonstrated that 39 downregulated and 22 upregulated genes interacted with pituitary functions and reproduction. Lastly, the interaction of the candidate lncRNA XR_001039544.4 and its targeted gene LHB were validated in sheep pituitary cells in vitro. LncRNA XR_001039544.4 and LHB showed high expression levels in the luteal phase in Hu sheep. LncRNA XR_001039544.4 is mainly located in the cytoplasm, as determined by FISH analysis, indicating that XR_001039544.4 might act as competing endogenous RNAs for miRNAs to regulate LHB. LncRNA XR_001039544.4 knockdown significantly inhibited LH secretion and cell proliferation. LncRNA XR_001039544.4 may regulate the secretion of LH in the luteal-phase pituitary gland via affecting cell proliferation. Taken together, these findings provided genome-wide lncRNA- and mRNA-expression profiles for the sheep pituitary gland between the follicular and luteal phases, thereby contributing to the elucidation of the molecular mechanisms of pituitary function.

show abstract

“…Additionally, in silico analysis in combination with the BMP15 promoter sequence showed that the -14 to -8 bp region of the BMP15 promoter is the primary regulatory target for the pituitary homebox 1 protein (PITX1) (49), in which two of the active binding regions were validated in vitro, namely, prom-G and prom-C constructs. PITX1 was reported to be associated with gonadotroph cell activation and estrogenic signals (58), and the estrus cycle-related estrogen signaling pathway was positively involved in POF traditional Chinese medicine treatment (59). Despite recent findings demonstrating the involvement of BMP15 mutation in POF, further studies elucidating the roles of modulators would lead to a better understanding of the disease pathogenesis.…”

Section: Bone Morphogenetic Protein 15 (Bmp15)mentioning

confidence: 99%

Current understanding of the genomic abnormities in premature ovarian failure: chance for early diagnosis and management

Yang

2023

Front. Med.

View full text Add to dashboard Cite

Premature ovarian failure (POF) is an insidious cause of female infertility and a devastating condition for women. POF also has a strong familial and heterogeneous genetic background. Management of POF is complicated by the variable etiology and presentation, which are generally characterized by abnormal hormone levels, gene instability and ovarian dysgenesis. To date, abnormal regulation associated with POF has been found in a small number of genes, including autosomal and sex chromosomal genes in folliculogenesis, granulosa cells, and oocytes. Due to the complex genomic contributions, ascertaining the exact causative mechanisms has been challenging in POF, and many pathogenic genomic characteristics have yet to be elucidated. However, emerging research has provided new insights into genomic variation in POF as well as novel etiological factors, pathogenic mechanisms and therapeutic intervention approaches. Meanwhile, scattered studies of transcriptional regulation revealed that ovarian cell function also depends on specific biomarker gene expression, which can influence protein activities, thus causing POF. In this review, we summarized the latest research and issues related to the genomic basis for POF and focused on insights gained from their biological effects and pathogenic mechanisms in POF. The present integrated studies of genomic variants, gene expression and related protein abnormalities were structured to establish the role of etiological genes associated with POF. In addition, we describe the design of some ongoing clinical trials that may suggest safe, feasible and effective approaches to improve the diagnosis and therapy of POF, such as Filgrastim, goserelin, resveratrol, natural plant antitoxin, Kuntai capsule et al. Understanding the candidate genomic characteristics in POF is beneficial for the early diagnosis of POF and provides appropriate methods for prevention and drug treatment. Additional efforts to clarify the POF genetic background are necessary and are beneficial for researchers and clinicians regarding genetic counseling and clinical practice. Taken together, recent genomic explorations have shown great potential to elucidate POF management in women and are stepping from the bench to the bedside.

show abstract

In vivo oestrogenic modulation of Egr1 and Pitx1 gene expression in female rat pituitary gland

Cited by 7 publications

References 62 publications

Silenced PITX1 promotes chemotherapeutic resistance to 5‑fluorocytosine and cisplatin in gastric cancer cells

Silenced PITX1 promotes chemotherapeutic resistance to 5‑fluorocytosine and cisplatin in gastric cancer cells

Comparative Transcriptomic Analysis of Hu Sheep Pituitary Gland Prolificacy at the Follicular and Luteal Phases

Current understanding of the genomic abnormities in premature ovarian failure: chance for early diagnosis and management

Contact Info

Product

Resources

About