Abstract:Optical coherence tomography is a powerful tool that can e ectively provide detail information of biological tissue structure. The axis resolution of optical coherence tomography has achieved sub-micrometer and we are interested in its ultrahigh resolution that can be practically utilized for clinical application. In this article, the basic system calibration is discussed; we systematically demonstrated the detail process in optimization of optical coherence tomography for accurately acquiring data. The systematic optimization for optical coherence tomography imaging is discussed here including system calibration and image post-processing.