In vivo noninvasive systemic myography of acute systemic vasoactivity in female pregnant mice

Abstract

Altered vasoactivity is a major characteristic of cardiovascular and oncological diseases, and many therapies are therefore targeted to the vasculature. Therapeutics which are selective for the diseased vasculature are ideal, but whole-body selectivity of a therapeutic is challenging to assess in practice. Vessel myography is used to determine the functional mechanisms and evaluate pharmacological responses of vascularly-targeted therapeutics. However, myography can only be performed on ex vivo sections of individual arteries. We have developed methods for implementation of spherical-view photoacoustic tomography for non-invasive and in vivo myography. Using photoacoustic tomography, we demonstrate the measurement of acute vascular reactivity in the systemic vasculature and the placenta of female pregnant mice in response to two vasodilators. Photoacoustic tomography simultaneously captures the significant acute vasodilation of major arteries and detects selective vasoactivity of the maternal-fetal vasculature. Photoacoustic tomography has the potential to provide invaluable preclinical information on vascular response that cannot be obtained by other established methods.

Deep learning acceleration of iterative model-based light fluence correction for photoacoustic tomography

“…Using multi-wavelength light excitation, multispectral PAT is able to effectively distinguish and quantitatively analyze different biological tissues given their optical absorption at different excitation wavelengths. Profiting from high imaging speed and centimeter-scale imaging depth, multispectral PAT has been widely applied in various preclinical studies [5] , [6] and clinical trials [7] , [8] .…”