In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy

Walther, Anders Runge; Ditzel, Nicholas; Kassem, Moustapha; Andersen, Morten Østergaard; Hedegaard, Martin A.B.

doi:10.1016/j.bbiosy.2022.100059

Biomaterials and Biosystems

2022

DOI: 10.1016/j.bbiosy.2022.100059

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In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy

Anders Runge Walther

Nicholas Ditzel

Moustapha Kassem

et al.

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“…In the context of biomedical applications, RS can provide insight into the composition of samples, as proteins, RNA/DNA, lipids, carbohydrates, and so forth produce different Raman spectra. This allows for the detection of changes in the relative concentration of such components in tissue as a result of pathological conditions. − With the development of miniaturized fiber-optic probes, it is possible to apply RS in vivo / in situ . ,,,− …”

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In Vivo Longitudinal Monitoring of Disease Progression in Inflammatory Arthritis Animal Models Using Raman Spectroscopy

et al. 2023

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Current techniques for monitoring disease progression and testing drug efficacy in animal models of inflammatory arthritis are either destructive, time-consuming, subjective, or require ionizing radiation. To accommodate this, we have developed a non-invasive and label-free optical system based on Raman spectroscopy for monitoring tissue alterations in rodent models of arthritis at the biomolecular level. To test different sampling geometries, the system was designed to collect both transmission and reflection mode spectra. Mice with collagen antibody-induced arthritis and controls were subject to in vivo Raman spectroscopy at the tibiotarsal joint every 3 days for 14 days. Raman-derived measures of bone content correlated well with micro-computed tomography bone mineral densities. This allowed for time-resolved quantitation of bone densities, which indicated gradual bone erosion in mice with arthritis. Inflammatory pannus formation, bone erosion, and bone marrow inflammation were confirmed by histological analysis. In addition, using library-based spectral decomposition, we quantified the progression of bone and soft tissue components. In general, the tissue components followed significantly different tendencies in mice developing arthritis compared to the control group in line with the histological analysis. In total, this demonstrates Raman spectroscopy as a versatile technique for monitoring alterations to both mineralized and soft tissues simultaneously in rodent models of musculoskeletal disorders. Furthermore, the technique presented herein allows for objective repeated within-animal measurements potentially refining and reducing the use of animals in research while improving the development of novel antiarthritic therapeutics.

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“… 13 − 21 With the development of miniaturized fiber-optic probes, 22 it is possible to apply RS in vivo / in situ . 15 , 16 , 19 , 23 − 25 …”

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confidence: 99%

In Vivo Longitudinal Monitoring of Disease Progression in Inflammatory Arthritis Animal Models Using Raman Spectroscopy

et al. 2023

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In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy

Cited by 1 publication

References 83 publications

In Vivo Longitudinal Monitoring of Disease Progression in Inflammatory Arthritis Animal Models Using Raman Spectroscopy

In Vivo Longitudinal Monitoring of Disease Progression in Inflammatory Arthritis Animal Models Using Raman Spectroscopy

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