In vivo near-infrared fluorescence imaging of Leishmania amazonensis expressing infrared fluorescence protein (iRFP) for real-time monitoring of cutaneous leishmaniasis in mice

Oliveira, Janaina Correia; Silva, Aline Caroline da; Oliveira, Renato Antônio dos Santos; Pereira, Valéria Rêgo Alves; Gil, Laura Helena Vega Gonzales

doi:10.1016/j.mimet.2016.08.003

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…Autoluminescence from tissues often interferes with the use of conventional GFP-like fluorescent proteins, including EGFP, DsRed and mCherry. However, when using near-IR fluorescence (NIRF) (from 650 to 900 nm), absorption of light by biological components such as haemoglobin, lipids and water is minimal, allowing light to reach its maximum depth of tissue penetration [24,25]. iRFP fluorescence labelling combines the advantages of luciferase for non-invasive, whole-animal imaging and GFP for cell imaging.…”

Section: Discussionmentioning

confidence: 99%

“…However, the effect and distribution of spontaneous fluorescence after injecting iRFP-labelled cells in vivo remained unclear. Other studies revealed the characteristics of cells injected subcutaneously into the head and feet respectively and presented the figures of the head and feet on in vivo images [15,25]. In these positions, the interfering substances were positioned low in the body, which can lead to low spontaneous fluorescence.…”

Section: Discussionmentioning

confidence: 99%

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AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging

et al. 2017

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Near-IR fluorescence (NIRF) imaging is a new technology using IR fluorescent protein (iRFP) gene labelling and is potentially useful for in vivo applications. In the present study, we expressed iRFP and the TNF-related apoptosis inducing ligand gene in mesenchymal stem cells (MSCs) using adeno-associated virus (AAV) and showed that iRFP-labelled MSCs can be detected by fluorescence microscopy. We injected mice with MSCs labelled with AAV-iRFP, which we were then able to detect by whole-animal NIRF imaging. Our technique provides a visualizable, convenient and sensitive platform for research on tracking the fate of transplanted MSC cells in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging

et al. 2017

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“…Low cytotoxicity of iRFP713 allowed generation of transgenic rat and mouse strains with high expression of this biomarker in all tissues, including nervous, cardiovascular, and digestive systems [5,64,69,75]. Besides mammals, the list of organisms demonstrating successful expression of NIR FP includes bacteria (Escherichia coli, Lactococcus lactis, several strains of Lactobacillus) [11 13, 22, 24, 26, 29, 59], yeast (Saccharomyces cerevisiae, Kluyveromyces lactis, Pichia pastoris) [56], Protozoa (several strains of Leishmania) [60,73], insects (Drosophila melanogaster) [17,24,64,84], nematodes (Caenorhabditis elegans) [22], and fish (Danio rerio) [24,25].…”

Section: Application Of Nir Fluorescent Biomarkers Reporters and Bimentioning

confidence: 99%

“…Labeling of viral particles and parasitic microorgan isms with NIR FPs offers the opportunity to investigate the spread of infection in a model organism and poten tially can be used for testing new therapeutic agents. Leishmania cells expressing iRFP713 [60,73], influenza A virus with introduced iRFP713 gene [77], and rabies virus with introduced iRFP670 and iRFP720 genes [102] were used for studying the pathologies with different eti ology in live mice.…”

Section: Application Of Nir Fluorescent Biomarkers Reporters and Bimentioning

confidence: 99%

Near-Infrared Fluorescent Proteins and Their Applications

Karasev

Stepanenko

Rumyantsev

et al. 2019

Biochemistry Moscow

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High transparency, low light scattering, and low autofluorescence of mammalian tissues in the near infrared (NIR) spectral range (~650 900 nm) open a possibility for in vivo imaging of biological processes at the micro and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP like fluores cent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that com bine fluorescence and bioluminescence methods with photoacoustic tomography.

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“…In future, labeled bacteria can be used as intra-intestinal factories for production and targeting of biologically active molecules. In parasitology, the introduction of iRFP713-expressing Leishmania parasites allowed their monitoring in mice and screening of medical drugs in vivo [ 150 ].…”

Section: Biological Applications Of Near-infrared Fluorescent Protmentioning

confidence: 99%

Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

Oliinyk

Chernov

Verkhusha

2017

IJMS

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Bacterial photoreceptors absorb light energy and transform it into intracellular signals that regulate metabolism. Bacterial phytochrome photoreceptors (BphPs), some cyanobacteriochromes (CBCRs) and allophycocyanins (APCs) possess the near-infrared (NIR) absorbance spectra that make them promising molecular templates to design NIR fluorescent proteins (FPs) and biosensors for studies in mammalian cells and whole animals. Here, we review structures, photochemical properties and molecular functions of several families of bacterial photoreceptors. We next analyze molecular evolution approaches to develop NIR FPs and biosensors. We then discuss phenotypes of current BphP-based NIR FPs and compare them with FPs derived from CBCRs and APCs. Lastly, we overview imaging applications of NIR FPs in live cells and in vivo. Our review provides guidelines for selection of existing NIR FPs, as well as engineering approaches to develop NIR FPs from the novel natural templates such as CBCRs.

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In vivo near-infrared fluorescence imaging of Leishmania amazonensis expressing infrared fluorescence protein (iRFP) for real-time monitoring of cutaneous leishmaniasis in mice

Cited by 14 publications

References 26 publications

AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging

AAV-iRFP labelling of human mesenchymal stem cells for near-infrared fluorescence imaging

Near-Infrared Fluorescent Proteins and Their Applications

Bacterial Phytochromes, Cyanobacteriochromes and Allophycocyanins as a Source of Near-Infrared Fluorescent Probes

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