In vivo multiphoton imaging of human skin: assessment of topical corticosteroid-induced epidermis atrophy and depigmentation

Madani, Hassan Ait El; Tancrède‐Bohin, Emmanuelle; Bensussan, Armand; Colonna, Anne; Dupuy, Alain; Bagot, M.; Pena, Ana‐Maria

doi:10.1117/1.jbo.17.2.026009

Cited by 39 publications

(36 citation statements)

References 41 publications

(45 reference statements)

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“…Most studies using in vivo skin multiphoton imaging have reported high-resolution images down to depths not exceeding 150 μm [13,10]. Our results explain the difficulty of maintaining satisfying image quality beyond the epidermis.…”

Section: Spatially-resolved Aberration Measurements On Human Skin mentioning

confidence: 64%

“…At intermediate depth (stratum granulosum, stratum spinosum and stratum basale, 20–60 μm ) shown on Fig. 3(b), bright melanin fluorescence (yellow arrow) and fluorescence from the cell cytoplasm (blue arrow) were visible, whereas fluorescence at larger depth (≥ 60 – 70 μ m) mainly arose from elastin in the dermal papillae (purple arrow), where a SHG signal from fibrillar collagen (shown in green) was also detected [11, 10]. …”

Section: Spatially-resolved Aberration Measurements On Human Skin mentioning

confidence: 99%

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3D resolved mapping of optical aberrations in thick tissues

Zeng¹,

Mahou²,

Schanne-Klein³

et al. 2012

Biomed. Opt. Express

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We demonstrate a simple method for mapping optical aberrations with 3D resolution within thick samples. The method relies on the local measurement of the variation in image quality with externally applied aberrations. We discuss the accuracy of the method as a function of the signal strength and of the aberration amplitude and we derive the achievable resolution for the resulting measurements. We then report on measured 3D aberration maps in human skin biopsies and mouse brain slices. From these data, we analyse the consequences of tissue structure and refractive index distribution on aberrations and imaging depth in normal and cleared tissue samples. The aberration maps allow the estimation of the typical aplanetism region size over which aberrations can be uniformly corrected. This method and data pave the way towards efficient correction strategies for tissue imaging applications.

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Section: Spatially-resolved Aberration Measurements On Human Skin mentioning

confidence: 64%

Section: Spatially-resolved Aberration Measurements On Human Skin mentioning

confidence: 99%

3D resolved mapping of optical aberrations in thick tissues

Zeng¹,

Mahou²,

Schanne-Klein³

et al. 2012

Biomed. Opt. Express

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“…; El Madani et al . ). The effects of a more short‐term use of 1% hydrocortisone creams on skin integrity needs further investigation.…”

Section: Discussionmentioning

confidence: 97%

Prophylaxis and management of acute radiation-induced skin toxicity: a survey of practice across Europe and the USA

O’Donovan

Coleman

Harris

et al. 2014

Eur J Cancer Care (Engl)

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Radiation-induced toxicity is a common adverse side effect of radiation therapy. Previous studies have demonstrated a lack of evidence to support common skincare advice for radiotherapy patients. The aim of the current study was to investigate the management of radiation-induced skin toxicity across Europe and the USA. Where previous surveys have focused on national practice or treatment of specific sites, the current study aimed to gain a broader representation of skincare practice. An anonymous online survey investigating various aspects of radiotherapy skincare management was distributed to departments across Europe and the USA (n = 181/737 responded i.e. 25%). The UK was excluded as a similar survey was carried out in 2011. The results highlight the lack of consistency in both the prevention and management of radiation-induced skin toxicity. Recommended products are often not based on evidence-based practice. Examples include the continued use of aqueous cream and gentian violet, as well as the recommendations on washing restrictions during treatment. To our knowledge, this is the most extensive survey to date on the current management of radiation-induced skin toxicity. This study highlights significant disparities between clinical practice and research-based evidence published in recent systematic reviews and guidelines. Ongoing large prospective randomised trials are urgently needed.

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“…Laser scanning confocal microscopy (LSCM) has allowed the visualization of keratinocytes in vivo, allowing changes in cell size to be observed in human patients (4). More recently, multiphoton microscopy (MPM) has been used to distinguish keratinocytes and collagen in the skin, allowing both visualization of the skin cell layers and measurements of skin thickness (16). However, in these studies, only structural information about the skin thickness and cell layers was obtained and any molecular or functional changes were largely ignored.…”

Section: Introductionmentioning

confidence: 99%

“…While in previous studies, the focus was mainly on identifying structural changes within epidermal keratinocytes (4, 16), the focus here is to instead study the metabolic environment of epidermal keratinocytes which may precede epidermal thinning. With this imaging system, subtle changes resulting from steroid treatment are observed that cannot otherwise be detected with LSCM or MPM alone.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal in vivo tracking of adverse effects following topical steroid treatment

Bower

Arp

Zhao

et al. 2016

Experimental Dermatology

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Topical steroids are known for their anti-inflammatory properties and are commonly prescribed to treat many adverse skin conditions such as eczema and psoriasis. While these treatments are known to be effective, adverse effects including skin atrophy are common. In this study, the progression of these effects is investigated in an in vivo mouse model using multimodal optical microscopy. Utilizing a system capable of performing two-photon excitation fluorescence microscopy (TPEF) of reduced nicotinamide adenine dinucleotide (NADH) to visualize the epidermal cell layers and second harmonic generation (SHG) microscopy to identify collagen in the dermis, these processes can be studied at the cellular level. Fluorescence lifetime imaging microscopy (FLIM) is also utilized to image intracellular NADH levels to obtain molecular information regarding metabolic activity following steroid treatment. In this study, fluticasone propionate (FP) treated, mometasone furoate (MF) treated, and untreated animals were imaged longitudinally using a custom-built multimodal optical microscope. Prolonged steroid treatment over the course of 21 days is shown to result in a significant increase in mean fluorescence lifetime of NADH, suggesting a faster rate of maturation of epidermal keratinocytes. Alterations to collagen organization and the structural microenvironment are also observed. These results give insight into the structural and biochemical processes of skin atrophy associated with prolonged steroid treatment.

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In vivo multiphoton imaging of human skin: assessment of topical corticosteroid-induced epidermis atrophy and depigmentation

Cited by 39 publications

References 41 publications

3D resolved mapping of optical aberrations in thick tissues

3D resolved mapping of optical aberrations in thick tissues

Prophylaxis and management of acute radiation-induced skin toxicity: a survey of practice across Europe and the USA

Longitudinal in vivo tracking of adverse effects following topical steroid treatment

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